A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia

P KELLY, M ARDIAN, G WARAMORI, Nicholas Anstey, H Syahrial, E TJITRA, I Bastian, G MAGUIRE, R Lumb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    SETTING: A district level tuberculosis (TB) control programme in Papua Province, Indonesia. OBJECTIVE: To determine the nature and extent of drug-resistant TB in newly diagnosed sputum smear-positive patients. METHODS: Sputum was collected from previously untreated smear-positive pulmonary TB patients diagnosed in the district over a 10-month period. Sputum specimens were processed and inoculated into a BACTEC MGIT960 tube. Isolates were identified by Ziehl-Neelsen staining, hybridisation with nucleic acid probes and biochemical investigations. Susceptibility testing was performed using the radiometric proportion method. Pyrazinamide testing was performed using the Wayne indirect method. RESULTS: One hundred and seven patients had sputum sent to a reference laboratory; 101 (94.4%) were culture-positive for Mycobacterium tuberculosis, with 87 (86.1%) fully sensitive to first-line anti-tuberculosis drugs. Two per cent were multidrug-resistant (MDR-TB) and 12 (11.9%) had other drug resistance. Each of the MDR-TB isolates was susceptible to amikacin, capreomycin, ciprofloxacin and para-aminosalicylic acid (PAS), but were resistant to rifabutin. One isolate was also resistant to ethionamide. CONCLUSIONS: MDR-TB is present in Indonesia but is not a major problem for TB control in this district. Generalisability to other districts in Indonesia, particularly large urban areas, needs to be confirmed by future studies. � 2006 The Union.
    Original languageEnglish
    Pages (from-to)167-171
    Number of pages5
    JournalInternational Journal of Tuberculosis and Lung Disease
    Volume10
    Issue number2
    Publication statusPublished - 2006

    Fingerprint

    Multidrug-Resistant Tuberculosis
    Indonesia
    Tuberculosis
    Sputum
    Pharmaceutical Preparations
    Capreomycin
    Ethionamide
    Aminosalicylic Acid
    Nucleic Acid Probes
    Rifabutin
    Pyrazinamide
    Amikacin
    Ciprofloxacin
    Pulmonary Tuberculosis
    Mycobacterium tuberculosis
    Drug Resistance
    Staining and Labeling

    Cite this

    KELLY, P ; ARDIAN, M ; WARAMORI, G ; Anstey, Nicholas ; Syahrial, H ; TJITRA, E ; Bastian, I ; MAGUIRE, G ; Lumb, R. / A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia. In: International Journal of Tuberculosis and Lung Disease. 2006 ; Vol. 10, No. 2. pp. 167-171.
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    title = "A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia",
    abstract = "SETTING: A district level tuberculosis (TB) control programme in Papua Province, Indonesia. OBJECTIVE: To determine the nature and extent of drug-resistant TB in newly diagnosed sputum smear-positive patients. METHODS: Sputum was collected from previously untreated smear-positive pulmonary TB patients diagnosed in the district over a 10-month period. Sputum specimens were processed and inoculated into a BACTEC MGIT960 tube. Isolates were identified by Ziehl-Neelsen staining, hybridisation with nucleic acid probes and biochemical investigations. Susceptibility testing was performed using the radiometric proportion method. Pyrazinamide testing was performed using the Wayne indirect method. RESULTS: One hundred and seven patients had sputum sent to a reference laboratory; 101 (94.4{\%}) were culture-positive for Mycobacterium tuberculosis, with 87 (86.1{\%}) fully sensitive to first-line anti-tuberculosis drugs. Two per cent were multidrug-resistant (MDR-TB) and 12 (11.9{\%}) had other drug resistance. Each of the MDR-TB isolates was susceptible to amikacin, capreomycin, ciprofloxacin and para-aminosalicylic acid (PAS), but were resistant to rifabutin. One isolate was also resistant to ethionamide. CONCLUSIONS: MDR-TB is present in Indonesia but is not a major problem for TB control in this district. Generalisability to other districts in Indonesia, particularly large urban areas, needs to be confirmed by future studies. � 2006 The Union.",
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    author = "P KELLY and M ARDIAN and G WARAMORI and Nicholas Anstey and H Syahrial and E TJITRA and I Bastian and G MAGUIRE and R Lumb",
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    KELLY, P, ARDIAN, M, WARAMORI, G, Anstey, N, Syahrial, H, TJITRA, E, Bastian, I, MAGUIRE, G & Lumb, R 2006, 'A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia', International Journal of Tuberculosis and Lung Disease, vol. 10, no. 2, pp. 167-171.

    A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia. / KELLY, P; ARDIAN, M; WARAMORI, G; Anstey, Nicholas; Syahrial, H; TJITRA, E; Bastian, I; MAGUIRE, G; Lumb, R.

    In: International Journal of Tuberculosis and Lung Disease, Vol. 10, No. 2, 2006, p. 167-171.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - A community-based TB drug susceptibility study in Mimika District, Papua Province, Indonesia

    AU - KELLY, P

    AU - ARDIAN, M

    AU - WARAMORI, G

    AU - Anstey, Nicholas

    AU - Syahrial, H

    AU - TJITRA, E

    AU - Bastian, I

    AU - MAGUIRE, G

    AU - Lumb, R

    PY - 2006

    Y1 - 2006

    N2 - SETTING: A district level tuberculosis (TB) control programme in Papua Province, Indonesia. OBJECTIVE: To determine the nature and extent of drug-resistant TB in newly diagnosed sputum smear-positive patients. METHODS: Sputum was collected from previously untreated smear-positive pulmonary TB patients diagnosed in the district over a 10-month period. Sputum specimens were processed and inoculated into a BACTEC MGIT960 tube. Isolates were identified by Ziehl-Neelsen staining, hybridisation with nucleic acid probes and biochemical investigations. Susceptibility testing was performed using the radiometric proportion method. Pyrazinamide testing was performed using the Wayne indirect method. RESULTS: One hundred and seven patients had sputum sent to a reference laboratory; 101 (94.4%) were culture-positive for Mycobacterium tuberculosis, with 87 (86.1%) fully sensitive to first-line anti-tuberculosis drugs. Two per cent were multidrug-resistant (MDR-TB) and 12 (11.9%) had other drug resistance. Each of the MDR-TB isolates was susceptible to amikacin, capreomycin, ciprofloxacin and para-aminosalicylic acid (PAS), but were resistant to rifabutin. One isolate was also resistant to ethionamide. CONCLUSIONS: MDR-TB is present in Indonesia but is not a major problem for TB control in this district. Generalisability to other districts in Indonesia, particularly large urban areas, needs to be confirmed by future studies. � 2006 The Union.

    AB - SETTING: A district level tuberculosis (TB) control programme in Papua Province, Indonesia. OBJECTIVE: To determine the nature and extent of drug-resistant TB in newly diagnosed sputum smear-positive patients. METHODS: Sputum was collected from previously untreated smear-positive pulmonary TB patients diagnosed in the district over a 10-month period. Sputum specimens were processed and inoculated into a BACTEC MGIT960 tube. Isolates were identified by Ziehl-Neelsen staining, hybridisation with nucleic acid probes and biochemical investigations. Susceptibility testing was performed using the radiometric proportion method. Pyrazinamide testing was performed using the Wayne indirect method. RESULTS: One hundred and seven patients had sputum sent to a reference laboratory; 101 (94.4%) were culture-positive for Mycobacterium tuberculosis, with 87 (86.1%) fully sensitive to first-line anti-tuberculosis drugs. Two per cent were multidrug-resistant (MDR-TB) and 12 (11.9%) had other drug resistance. Each of the MDR-TB isolates was susceptible to amikacin, capreomycin, ciprofloxacin and para-aminosalicylic acid (PAS), but were resistant to rifabutin. One isolate was also resistant to ethionamide. CONCLUSIONS: MDR-TB is present in Indonesia but is not a major problem for TB control in this district. Generalisability to other districts in Indonesia, particularly large urban areas, needs to be confirmed by future studies. � 2006 The Union.

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    KW - aminosalicylic acid

    KW - capreomycin

    KW - ciprofloxacin

    KW - ethionamide

    KW - pyrazinamide

    KW - rifabutin

    KW - tuberculostatic agent

    KW - adolescent

    KW - adult

    KW - aged

    KW - article

    KW - drug sensitivity

    KW - drug tolerance

    KW - female

    KW - human

    KW - hybridization

    KW - Indonesia

    KW - laboratory test

    KW - lung tuberculosis

    KW - major clinical study

    KW - male

    KW - multidrug resistance

    KW - Mycobacterium tuberculosis

    KW - nucleic acid probe

    KW - priority journal

    KW - sputum smear

    KW - Adolescent

    KW - Adult

    KW - Aged

    KW - Antitubercular Agents

    KW - Drug Resistance, Bacterial

    KW - Female

    KW - Humans

    KW - Male

    KW - Middle Aged

    KW - Population Surveillance

    KW - Prevalence

    KW - Retrospective Studies

    KW - Tuberculosis, Multidrug-Resistant

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    JO - International Journal of Tuberculosis and Lung Disease

    JF - International Journal of Tuberculosis and Lung Disease

    SN - 1027-3719

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    ER -