A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children

Christine Wigger, Peter Stanley Morris, Matthew Stevens, Heidi Smith-Vaughan, Kim Hare, Jemima Beissbarth, Amanda Jane Leach

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.

    Original languageEnglish
    Pages (from-to)47-49
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume157
    DOIs
    Publication statusPublished - 1 Feb 2019

    Fingerprint

    Nylons
    Bacteria
    Haemophilus influenzae
    Streptococcus pneumoniae
    Moraxella (Branhamella) catarrhalis
    Growth
    rayon
    Costs and Cost Analysis
    Population

    Cite this

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    title = "A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children",
    abstract = "This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90{\%} cf. 74{\%}, p = 0.375), H. influenzae (79{\%} cf. 74{\%}, p = 1.00) and M. catarrhalis (79{\%} cf. 74{\%}, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.",
    keywords = "Flocked nylon swabs, Haemophilus influenzae, Moraxella catarrhalis, Nasal, Nasopharyngeal, Rayon swabs, Streptococcus pneumoniae",
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    A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children. / Wigger, Christine; Morris, Peter Stanley; Stevens, Matthew; Smith-Vaughan, Heidi; Hare, Kim; Beissbarth, Jemima; Leach, Amanda Jane.

    In: Journal of Microbiological Methods, Vol. 157, 01.02.2019, p. 47-49.

    Research output: Contribution to journalArticleResearchpeer-review

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    T1 - A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children

    AU - Wigger, Christine

    AU - Morris, Peter Stanley

    AU - Stevens, Matthew

    AU - Smith-Vaughan, Heidi

    AU - Hare, Kim

    AU - Beissbarth, Jemima

    AU - Leach, Amanda Jane

    PY - 2019/2/1

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    N2 - This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.

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    KW - Haemophilus influenzae

    KW - Moraxella catarrhalis

    KW - Nasal

    KW - Nasopharyngeal

    KW - Rayon swabs

    KW - Streptococcus pneumoniae

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    JF - Journal of Microbiological Methods

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