A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children

Christine Wigger, Peter Stanley Morris, Matthew Stevens, Heidi Smith-Vaughan, Kim Hare, Jemima Beissbarth, Amanda Jane Leach

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.

Original languageEnglish
Pages (from-to)47-49
Number of pages3
JournalJournal of Microbiological Methods
Volume157
Issue numberFebruary
DOIs
Publication statusPublished - 1 Feb 2019

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Nylons
Bacteria
Haemophilus influenzae
Streptococcus pneumoniae
Moraxella (Branhamella) catarrhalis
Growth
rayon
Costs and Cost Analysis
Population

Cite this

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title = "A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children",
abstract = "This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90{\%} cf. 74{\%}, p = 0.375), H. influenzae (79{\%} cf. 74{\%}, p = 1.00) and M. catarrhalis (79{\%} cf. 74{\%}, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.",
keywords = "Flocked nylon swabs, Haemophilus influenzae, Moraxella catarrhalis, Nasal, Nasopharyngeal, Rayon swabs, Streptococcus pneumoniae",
author = "Christine Wigger and Morris, {Peter Stanley} and Matthew Stevens and Heidi Smith-Vaughan and Kim Hare and Jemima Beissbarth and Leach, {Amanda Jane}",
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T1 - A comparison of flocked nylon swabs and non-flocked rayon swabs for detection of respiratory bacteria in nasopharyngeal carriage in Australian Indigenous children

AU - Wigger, Christine

AU - Morris, Peter Stanley

AU - Stevens, Matthew

AU - Smith-Vaughan, Heidi

AU - Hare, Kim

AU - Beissbarth, Jemima

AU - Leach, Amanda Jane

PY - 2019/2/1

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N2 - This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.

AB - This study compared flocked (nylon) swabs and (non-flocked) rayon swabs for the detection of Streptococcus pneumoniae, Haemophilus influenzae and Moraxella catarrhalis in nasopharyngeal samples from 20 enrolled Indigenous children under the age of 6 years living in remote Australian Aboriginal communities, and determined which swab the child or parent perceived to be more comfortable. There was no evidence of a significant difference between flocked and rayon swabs in the recovery of common respiratory bacteria. Rayon swabs detected presence of S. pneumoniae (90% cf. 74%, p = 0.375), H. influenzae (79% cf. 74%, p = 1.00) and M. catarrhalis (79% cf. 74%, p = 1.00) at higher rates than the flocked swabs. Analysis of semi-quantitative growth scores also showed no significant differences in either the ranked distributions or medians. Rayon swabs median semi-quantitative growth scores were higher for S. pneumoniae (4 [IQR 1–5] cf. 3 [IQR 0–6], p = 0.699), and H. influenzae (2 [IQR1–5] cf. 1 [IQR0–5], p = 0.946). Sixty percent of participants preferred samples to be taken with flocked swabs. This study demonstrates that microbiological outcomes are not compromised when using flocked or rayon swabs in respiratory bacterial carriage studies in this population. Therefore, cost, methodological consistency across studies, and participant preference can be considered when choosing swab type.

KW - Flocked nylon swabs

KW - Haemophilus influenzae

KW - Moraxella catarrhalis

KW - Nasal

KW - Nasopharyngeal

KW - Rayon swabs

KW - Streptococcus pneumoniae

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U2 - 10.1016/j.mimet.2018.12.013

DO - 10.1016/j.mimet.2018.12.013

M3 - Article

VL - 157

SP - 47

EP - 49

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

SN - 0167-7012

IS - February

ER -