A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

Rama Jayaraj; Belinda Hales; Linda Viberg; Susan Pizzutto; Deborah Holt; Jennifer M Rolland; Robyn O'hehir; Bart Currie; Shelley Walton

doi:10.1016/j.diagmicrobio.2011.09.007

A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

Rama Jayaraj, Belinda Hales, Linda Viberg, Susan Pizzutto, Deborah Holt, Jennifer M Rolland, Robyn O'hehir, Bart Currie, Shelley Walton

Research output: Contribution to journal › Article › peer-review

Abstract

Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. � 2011 Elsevier Inc.

Original language	English
Pages (from-to)	403-407
Number of pages	5
Journal	Diagnostic Microbiology and Infectious Disease
Volume	71
DOIs	https://doi.org/10.1016/j.diagmicrobio.2011.09.007
Publication status	Published - 2011

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10.1016/j.diagmicrobio.2011.09.007

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title = "A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei",

abstract = "Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. � 2011 Elsevier Inc.",

keywords = "immunoglobulin E, lanthanide, recombinant allergen, recombinant antigen, recombinant Der p 14 antigen, recombinant Sar s 14.3 antigen, unclassified drug, antibody specificity, antigen binding, article, controlled study, cross reaction, Dermatophagoides, diagnostic accuracy, diagnostic value, human, immunofluorescence test, major clinical study, nonhuman, priority journal, protein blood level, quantitative analysis, Sarcoptes scabiei, scabies, sensitivity and specificity, Allergens, Animals, Cross Reactions, Diagnostic Tests, Routine, Humans, Immunoassay, Immunoglobulin E, Parasitology, Recombinant Proteins, Scabies, Sensitivity and Specificity",

author = "Rama Jayaraj and Belinda Hales and Linda Viberg and Susan Pizzutto and Deborah Holt and Rolland, {Jennifer M} and Robyn O'hehir and Bart Currie and Shelley Walton",

year = "2011",

doi = "10.1016/j.diagmicrobio.2011.09.007",

language = "English",

volume = "71",

pages = "403--407",

journal = "Diagnostic Microbiology and Infectious Disease",

issn = "0732-8893",

publisher = "Elsevier",

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TY - JOUR

T1 - A diagnostic test for scabies

T2 - IgE specificity for a recombinant allergen of Sarcoptes scabiei

AU - Jayaraj, Rama

AU - Hales, Belinda

AU - Viberg, Linda

AU - Pizzutto, Susan

AU - Holt, Deborah

AU - Rolland, Jennifer M

AU - O'hehir, Robyn

AU - Currie, Bart

AU - Walton, Shelley

PY - 2011

Y1 - 2011

N2 - Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. � 2011 Elsevier Inc.

AB - Scabies infestations are difficult to diagnose clinically and current serologic tests have less than 50% accuracy. To develop more reliable diagnosis of scabies, specific IgE antibodies to a major scabies antigen recombinant Sar s 14.3 (rSar s 14.3) were measured in 140 plasma samples from scabies-infested and control subject groups using dissociation-enhanced lanthanide fluorescent immunoassays (DELFIA). Levels of rSar s 14.3-specific IgE were quantified, and cross-reactivity with its house dust mite homologue, Der p 14, was assessed. The rSar s 14.3 DELFIA showed excellent diagnostic capability, with 100% sensitivity and 93.75% specificity for distinguishing subjects with current scabies infestation from control, uninfested subjects. Recombinant Der p 14 preparation was ineffective at inhibiting IgE binding to rSar s 14.3. This study shows that quantification of levels of IgE antibody to rSar s 14.3 is a highly sensitive method for diagnosis of scabies infestation in clinical practice. � 2011 Elsevier Inc.

KW - immunoglobulin E

KW - lanthanide

KW - recombinant allergen

KW - recombinant antigen

KW - recombinant Der p 14 antigen

KW - recombinant Sar s 14.3 antigen

KW - unclassified drug

KW - antibody specificity

KW - antigen binding

KW - article

KW - controlled study

KW - cross reaction

KW - Dermatophagoides

KW - diagnostic accuracy

KW - diagnostic value

KW - human

KW - immunofluorescence test

KW - major clinical study

KW - nonhuman

KW - priority journal

KW - protein blood level

KW - quantitative analysis

KW - Sarcoptes scabiei

KW - scabies

KW - sensitivity and specificity

KW - Allergens

KW - Animals

KW - Cross Reactions

KW - Diagnostic Tests, Routine

KW - Humans

KW - Immunoassay

KW - Immunoglobulin E

KW - Parasitology

KW - Recombinant Proteins

KW - Scabies

KW - Sensitivity and Specificity

U2 - 10.1016/j.diagmicrobio.2011.09.007

DO - 10.1016/j.diagmicrobio.2011.09.007

M3 - Article

C2 - 22018936

VL - 71

SP - 403

EP - 407

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

SN - 0732-8893

ER -

A diagnostic test for scabies: IgE specificity for a recombinant allergen of Sarcoptes scabiei

Abstract

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