A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions

Apichai Tuanyok, Raymond Auerbach, Thomas Brettin, David Bruce, A Munk, John Chris Detter, Talima Pearson, Heidie Hornstra, R Sermswan, Vanaporn Wuthiekanun, S PEACOCK, Bart Currie, Paul S Keim, David M Wagner

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model. Copyright � 2007, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)9044-9049
    Number of pages6
    JournalJournal of Bacteriology
    Volume189
    Issue number24
    Publication statusPublished - 2007

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    Burkholderia pseudomallei
    Horizontal Gene Transfer
    Flagella
    Chemotaxis
    Multigene Family
    Genome
    Melioidosis
    Thailand
    Microbiology
    Animal Models
    DNA

    Cite this

    Tuanyok, A., Auerbach, R., Brettin, T., Bruce, D., Munk, A., Detter, J. C., ... Wagner, D. M. (2007). A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions. Journal of Bacteriology, 189(24), 9044-9049.
    Tuanyok, Apichai ; Auerbach, Raymond ; Brettin, Thomas ; Bruce, David ; Munk, A ; Detter, John Chris ; Pearson, Talima ; Hornstra, Heidie ; Sermswan, R ; Wuthiekanun, Vanaporn ; PEACOCK, S ; Currie, Bart ; Keim, Paul S ; Wagner, David M. / A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 24. pp. 9044-9049.
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    abstract = "Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model. Copyright � 2007, American Society for Microbiology. All Rights Reserved.",
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    author = "Apichai Tuanyok and Raymond Auerbach and Thomas Brettin and David Bruce and A Munk and Detter, {John Chris} and Talima Pearson and Heidie Hornstra and R Sermswan and Vanaporn Wuthiekanun and S PEACOCK and Bart Currie and Keim, {Paul S} and Wagner, {David M}",
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    Tuanyok, A, Auerbach, R, Brettin, T, Bruce, D, Munk, A, Detter, JC, Pearson, T, Hornstra, H, Sermswan, R, Wuthiekanun, V, PEACOCK, S, Currie, B, Keim, PS & Wagner, DM 2007, 'A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions', Journal of Bacteriology, vol. 189, no. 24, pp. 9044-9049.

    A Horizontal Gene Transfer Event Defines Two Distinct Groups within Burkholderia pseudomallei That Have Dissimilar Geographic Distributions. / Tuanyok, Apichai; Auerbach, Raymond; Brettin, Thomas; Bruce, David; Munk, A; Detter, John Chris; Pearson, Talima; Hornstra, Heidie; Sermswan, R; Wuthiekanun, Vanaporn; PEACOCK, S; Currie, Bart; Keim, Paul S; Wagner, David M.

    In: Journal of Bacteriology, Vol. 189, No. 24, 2007, p. 9044-9049.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

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    AU - Tuanyok, Apichai

    AU - Auerbach, Raymond

    AU - Brettin, Thomas

    AU - Bruce, David

    AU - Munk, A

    AU - Detter, John Chris

    AU - Pearson, Talima

    AU - Hornstra, Heidie

    AU - Sermswan, R

    AU - Wuthiekanun, Vanaporn

    AU - PEACOCK, S

    AU - Currie, Bart

    AU - Keim, Paul S

    AU - Wagner, David M

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    N2 - Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model. Copyright � 2007, American Society for Microbiology. All Rights Reserved.

    AB - Burkholderia pseudomallei is the etioiogic agent of melioidosis. Many disease manifestations are associated with melioidosis, and the mechanisms causing this variation are unknown; genomic differences among strains offer one explanation. We compared the genome sequences of two strains of B. pseudomallei: the original reference strain K96243 from Thailand and strain MSHR305 from Australia. We identified a variable homologous region between the two strains. This region was previously identified in comparisons of the genome of B. pseudomallei strain K96243 with the genome of strain E264 from the closely related B. thailandensis. In that comparison, K96243 was shown to possess a horizontally acquired rersinia-like fimbrial (YLF) gene cluster. Here, we show that the homologous genomic region in B. pseudomallei strain 305 is similar to that previously identified in B. thailandensis strain E264. We have named this region in B. pseudomallei strain 305 the B. thailandensis-like flagellum and chemotaxis (BTFC) gene cluster. We screened for these different genomic components across additional genome sequences and 571 B. pseudomallei DNA extracts obtained from regions of endemicity. These alternate genomic states define two distinct groups within B. pseudomallei: all strains contained either the BTFC gene cluster (group BTFC) or the YLF gene cluster (group YLF). These two groups have distinct geographic distributions: group BTFC is dominant in Australia, and group YLF is dominant in Thailand and elsewhere. In addition, clinical isolates are more likely to belong to group YLF, whereas environmental isolates are more likely to belong to group BTFC. These groups should be further characterized in an animal model. Copyright � 2007, American Society for Microbiology. All Rights Reserved.

    KW - DNA

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    KW - bacterial genome

    KW - bacterial strain

    KW - bacterium isolate

    KW - Burkholderia pseudomallei

    KW - chemotaxis

    KW - controlled study

    KW - flagellum

    KW - gene cluster

    KW - gene transfer

    KW - genetic screening

    KW - geographic distribution

    KW - nonhuman

    KW - priority journal

    KW - Australia

    KW - Chromosomes, Bacterial

    KW - DNA, Bacterial

    KW - Environmental Microbiology

    KW - Epidemiology, Molecular

    KW - Evolution, Molecular

    KW - Gene Transfer, Horizontal

    KW - Genotype

    KW - Humans

    KW - Melioidosis

    KW - Molecular Sequence Data

    KW - Multigene Family

    KW - Sequence Analysis, DNA

    KW - Sequence Homology

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    KW - Animalia

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    SP - 9044

    EP - 9049

    JO - Journal of Bacteriology

    JF - Journal of Bacteriology

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