TY - JOUR
T1 - A method for the isolation of RNA from Streptococcus salivarius and its application to the transcriptional analysis of the gtfJK locus
AU - Simpson, Christine L.
AU - Giffard, Philip M.
AU - Jacques, Nicholas A.
PY - 1993/3/15
Y1 - 1993/3/15
N2 - The glucosyltransferases from oral streptococci cleave sucrose and polymerize the glucose moieties. In Streptococcus salivarius ATCC 25975, two glucosyltransferase-encoding genes, gtfJ and gtfK, are closely linked and transcribed in the same direction. A procedure for the isolation of intact RNA from this organism was devised. The procedure incorporated a high-temperature mutanolysin treatment and selective precipitation by LiCl. The RNA was subject to Northern hybridization and RNase protection assays and it was concluded that the two genes are transcribed separately. A potential factor-independent transcription terminator was located in the intergenic region.
AB - The glucosyltransferases from oral streptococci cleave sucrose and polymerize the glucose moieties. In Streptococcus salivarius ATCC 25975, two glucosyltransferase-encoding genes, gtfJ and gtfK, are closely linked and transcribed in the same direction. A procedure for the isolation of intact RNA from this organism was devised. The procedure incorporated a high-temperature mutanolysin treatment and selective precipitation by LiCl. The RNA was subject to Northern hybridization and RNase protection assays and it was concluded that the two genes are transcribed separately. A potential factor-independent transcription terminator was located in the intergenic region.
KW - Glucosyltransferase
KW - RNA
KW - Streptococcus salivarius
KW - Transcriptional analysis
UR - http://www.scopus.com/inward/record.url?scp=0027449994&partnerID=8YFLogxK
U2 - 10.1111/j.1574-6968.1993.tb06079.x
DO - 10.1111/j.1574-6968.1993.tb06079.x
M3 - Article
C2 - 7682529
AN - SCOPUS:0027449994
VL - 108
SP - 93
EP - 97
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 1
ER -