A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Janessa Pickering; Michael Binks; Jemima Beissbarth; Kim Hare; Lea-Ann S Kirkham; Heidi Smith-Vaughan

doi:10.1128/JCM.02191-13

A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Janessa Pickering, Michael Binks, Jemima Beissbarth, Kim Hare, Lea-Ann S Kirkham, Heidi Smith-Vaughan

Child and Maternal Health

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.

Original language	English
Pages (from-to)	663-667
Number of pages	5
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	2
DOIs	https://doi.org/10.1128/JCM.02191-13
Publication status	Published - Feb 2014

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10.1128/JCM.02191-13

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abstract = "We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials. ",

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AU - Beissbarth, Jemima

AU - Hare, Kim

AU - Kirkham, Lea-Ann S

AU - Smith-Vaughan, Heidi

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KW - nucleotide sequence

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A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Abstract

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