A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus

Janessa Pickering, Michael Binks, Jemima Beissbarth, Kim Hare, Lea-Ann S Kirkham, Heidi Smith-Vaughan

    Research output: Contribution to journalArticle

    Abstract

    We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.
    Original languageEnglish
    Pages (from-to)663-667
    Number of pages5
    JournalJournal of Clinical Microbiology
    Volume52
    Issue number2
    DOIs
    Publication statusPublished - Feb 2014

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