We have developed a PCR-high-resolution melt (PCR-HRM) assay to discriminate nontypeable Haemophilus influenzae (NTHi) colonies from Haemophilus haemolyticus. This method is rapid and robust, with 96% sensitivity and 92% specificity compared to the hpd#3 assay. PCR-HRM is ideal for high-throughput screening for NTHi surveillance and clinical trials.
Pickering, J., Binks, M., Beissbarth, J., Hare, K., Kirkham, L-A. S., & Smith-Vaughan, H. (2014). A PCR-High-Resolution Melt Assay for Rapid Differentiation of Nontypeable Haemophilus influenzae and Haemophilus haemolyticus. Journal of Clinical Microbiology, 52(2), 663-667. https://doi.org/10.1128/JCM.02191-13