A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa

Dhammika Leshan Wannigama, Cameron Hurst, Parichart Hongsing, Lachlan Pearson, Thammakorn Saethang, Naphat Chantaravisoot, Uthaibhorn Singkham-In, Sirirat Luk-In, Robin James Storer, Tanittha Chatsuwan

Research output: Contribution to journalArticlepeer-review

Abstract

Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalAnnals of Clinical Microbiology and Antimicrobials
Volume19
Issue number1
DOIs
Publication statusPublished - 13 Mar 2020
Externally publishedYes

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