TY - JOUR
T1 - A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa
AU - Wannigama, Dhammika Leshan
AU - Hurst, Cameron
AU - Hongsing, Parichart
AU - Pearson, Lachlan
AU - Saethang, Thammakorn
AU - Chantaravisoot, Naphat
AU - Singkham-In, Uthaibhorn
AU - Luk-In, Sirirat
AU - Storer, Robin James
AU - Chatsuwan, Tanittha
N1 - Funding Information:
This work was supported by a grant from the 90th Year Anniversary Ratcha‑ dapiseksompotch Endowment Fund from the Faculty of Medicine and Gradu‑ ate School, Chulalongkorn University, Bangkok, Thailand (batch No. 39 (2/61)). Dhammika Leshan Wannigama was supported under the 100th Anniversary Chulalongkorn University Fund for Doctoral Scholarships, The University of Western Australia for Cross Institutional Exchange Doctoral Fellowship and Graduate School, Chulalongkorn University, for Overseas Research Experience Scholarship. The sponsor(s) had no role in study design; in the collection, analysis and interpretation of data; in the writing of the report; or in the deci‑ sion to submit the article for publication.
Publisher Copyright:
© 2020 The Author(s).
PY - 2020/3/13
Y1 - 2020/3/13
N2 - Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.
AB - Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.
KW - Antibiofilm
KW - Antimicrobial susceptibility
KW - Biofilm infections
KW - Biofilms
KW - Chronic bacterial infections
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85081976448&partnerID=8YFLogxK
U2 - 10.1186/s12941-020-00350-6
DO - 10.1186/s12941-020-00350-6
M3 - Article
C2 - 32169075
VL - 19
SP - 1
EP - 8
JO - Annals of Clinical Microbiology and Antimicrobials
JF - Annals of Clinical Microbiology and Antimicrobials
SN - 1476-0711
IS - 1
ER -