A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi

S Britton, Qin Cheng, Matthew Grigg, Timothy William, Nicholas Anstey, James McCarthy

    Research output: Contribution to journalArticleResearchpeer-review

    10 Downloads (Pure)

    Abstract

    The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.

    © The American Society of Tropical Medicine and Hygiene
    Original languageEnglish
    Pages (from-to)120-122
    Number of pages3
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume95
    Issue number1
    DOIs
    Publication statusPublished - Jul 2016

    Fingerprint

    Plasmodium knowlesi
    Point-of-Care Systems
    Routine Diagnostic Tests
    Malaria
    Parasites
    Polymerase Chain Reaction
    Plasmodium
    Malaysia
    Diagnostic Errors
    Antigens

    Cite this

    @article{62457c69475d47f3a2bc75250b4a41c8,
    title = "A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi",
    abstract = "The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96{\%} for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.{\circledC} The American Society of Tropical Medicine and Hygiene",
    author = "S Britton and Qin Cheng and Matthew Grigg and Timothy William and Nicholas Anstey and James McCarthy",
    year = "2016",
    month = "7",
    doi = "10.4269/ajtmh.15-0670",
    language = "English",
    volume = "95",
    pages = "120--122",
    journal = "The American Journal of Tropical Medicine and Hygiene",
    issn = "0002-9637",
    publisher = "American Society of Tropical Medicine and Hygiene",
    number = "1",

    }

    A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi. / Britton, S; Cheng, Qin; Grigg, Matthew; William, Timothy; Anstey, Nicholas; McCarthy, James.

    In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 1, 07.2016, p. 120-122.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi

    AU - Britton, S

    AU - Cheng, Qin

    AU - Grigg, Matthew

    AU - William, Timothy

    AU - Anstey, Nicholas

    AU - McCarthy, James

    PY - 2016/7

    Y1 - 2016/7

    N2 - The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.© The American Society of Tropical Medicine and Hygiene

    AB - The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.© The American Society of Tropical Medicine and Hygiene

    UR - http://www.scopus.com/inward/record.url?scp=84977663240&partnerID=8YFLogxK

    U2 - 10.4269/ajtmh.15-0670

    DO - 10.4269/ajtmh.15-0670

    M3 - Article

    VL - 95

    SP - 120

    EP - 122

    JO - The American Journal of Tropical Medicine and Hygiene

    JF - The American Journal of Tropical Medicine and Hygiene

    SN - 0002-9637

    IS - 1

    ER -