A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi

S Britton, Qin Cheng, Matthew Grigg, Timothy William, Nicholas Anstey, James McCarthy

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Abstract

The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.

© The American Society of Tropical Medicine and Hygiene
Original languageEnglish
Pages (from-to)120-122
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume95
Issue number1
DOIs
Publication statusPublished - Jul 2016

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Plasmodium knowlesi
Point-of-Care Systems
Routine Diagnostic Tests
Malaria
Parasites
Polymerase Chain Reaction
Plasmodium
Malaysia
Diagnostic Errors
Antigens

Cite this

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title = "A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi",
abstract = "The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96{\%} for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.{\circledC} The American Society of Tropical Medicine and Hygiene",
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A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi. / Britton, S; Cheng, Qin; Grigg, Matthew; William, Timothy; Anstey, Nicholas; McCarthy, James.

In: American Journal of Tropical Medicine and Hygiene, Vol. 95, No. 1, 07.2016, p. 120-122.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi

AU - Britton, S

AU - Cheng, Qin

AU - Grigg, Matthew

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AU - Anstey, Nicholas

AU - McCarthy, James

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AB - The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.© The American Society of Tropical Medicine and Hygiene

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