TY - JOUR
T1 - A sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay for the detection of plasmodium Knowlesi
AU - Britton, S
AU - Cheng, Qin
AU - Grigg, Matthew
AU - William, Timothy
AU - Anstey, Nicholas
AU - McCarthy, James
PY - 2016/7
Y1 - 2016/7
N2 - The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.© The American Society of Tropical Medicine and Hygiene
AB - The simian parasite Plasmodium knowlesi is now the commonest cause of malaria in Malaysia and can rapidly cause severe and fatal malaria. However, microscopic misdiagnosis of Plasmodium species is common, rapid antigen detection tests remain insufficiently sensitive and confirmation of P. knowlesi requires polymerase chain reaction (PCR). Thus available point-of-care diagnostic tests are inadequate. This study reports the development of a simple, sensitive, colorimetric, high-throughput loop-mediated isothermal amplification assay (HtLAMP) diagnostic test using novel primers for the detection of P. knowlesi. This assay is able to detect 0.2 parasites/μL, and compared with PCR has a sensitivity of 96% for the detection of P. knowlesi, making it a potentially field-applicable point-of-care diagnostic tool.© The American Society of Tropical Medicine and Hygiene
UR - http://www.scopus.com/inward/record.url?scp=84977663240&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.15-0670
DO - 10.4269/ajtmh.15-0670
M3 - Article
C2 - 27162264
VL - 95
SP - 120
EP - 122
JO - The American Journal of Tropical Medicine and Hygiene
JF - The American Journal of Tropical Medicine and Hygiene
SN - 0002-9637
IS - 1
ER -