aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon

Jan Bell, John Turnidge, Patiyan Andersson

    Research output: Contribution to journalArticle

    Abstract

    We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
    Original languageEnglish
    Pages (from-to)1378-1380
    Number of pages3
    JournalAntimicrobial Agents and Chemotherapy
    Volume54
    Issue number3
    Early online date14 Dec 2009
    DOIs
    Publication statusPublished - Mar 2010

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