We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
Bell, J., Turnidge, J., & Andersson, P. (2010). aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon. Antimicrobial Agents and Chemotherapy, 54(3), 1378-1380. https://doi.org/10.1128/AAC.01476-09