aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon

Jan Bell; John Turnidge; Patiyan Andersson

doi:10.1128/AAC.01476-09

aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon

Jan Bell, John Turnidge, Patiyan Andersson

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.

Original language	English
Pages (from-to)	1378-1380
Number of pages	3
Journal	Antimicrobial Agents and Chemotherapy
Volume	54
Issue number	3
Early online date	14 Dec 2009
DOIs	https://doi.org/10.1128/AAC.01476-09
Publication status	Published - Mar 2010

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title = "aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon",

abstract = "We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.",

author = "Jan Bell and John Turnidge and Patiyan Andersson",

year = "2010",

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doi = "10.1128/AAC.01476-09",

language = "English",

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pages = "1378--1380",

journal = "Antimicrobial Agents and Chemotherapy",

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publisher = "American Society for Microbiology",

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AU - Bell, Jan

AU - Turnidge, John

AU - Andersson, Patiyan

PY - 2010/3

Y1 - 2010/3

N2 - We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.

AB - We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.

U2 - 10.1128/AAC.01476-09

DO - 10.1128/AAC.01476-09

M3 - Article

VL - 54

SP - 1378

EP - 1380

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

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