Abstract
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
| Original language | English |
|---|---|
| Pages (from-to) | 1378-1380 |
| Number of pages | 3 |
| Journal | Antimicrobial Agents and Chemotherapy |
| Volume | 54 |
| Issue number | 3 |
| Early online date | 14 Dec 2009 |
| DOIs | |
| Publication status | Published - Mar 2010 |
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Bell, J., Turnidge, J., & Andersson, P. (2010). aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon. Antimicrobial Agents and Chemotherapy, 54(3), 1378-1380. https://doi.org/10.1128/AAC.01476-09