Abstract
We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6′)-Ib-cr that is capable of distinguishing between the two genetic aac(6′)-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon.
Original language | English |
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Pages (from-to) | 1378-1380 |
Number of pages | 3 |
Journal | Antimicrobial Agents and Chemotherapy |
Volume | 54 |
Issue number | 3 |
Early online date | 14 Dec 2009 |
DOIs | |
Publication status | Published - Mar 2010 |