TY - JOUR
T1 - Accuracy of enzyme-linked immunosorbent assay using crude and purified antigens for serodiagnosis of melioidosis
AU - Chantratita, Narisara
AU - Wuthiekanun, Vanaporn
AU - Thanwisai, Aunchalee
AU - Limmathurotsakul, Direk
AU - Cheng, Allen
AU - Chierakul, Wirongrong
AU - Day, Nicholas P. J.
AU - Peacock, Sharon J.
PY - 2007
Y1 - 2007
N2 - Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia Pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
AB - Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia Pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).
KW - Burkholderia pseudomallai
KW - northeast Thailand
KW - indirect hemagglutination
KW - PSEUDOMONAS-PSEUDOMALLEI
KW - serological diagnosis
KW - area
KW - lipopolysaccharide
KW - polysaccaride
KW - antibodies
KW - virulence
U2 - 10.1128/CVI.00289-06
DO - 10.1128/CVI.00289-06
M3 - Comment/debate
VL - 14
SP - 110
EP - 113
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
SN - 1071-412X
IS - 1
ER -