Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles

Alice C-H Chen , Olga M. Pena, Hendrik J. Nel, Stephanie Yerkovich, Anne Chang, Katherine J. Baines, Peter G. Gibson, Helen L. Petsky, Susan J. Pizzutto, Sandra Hodge, Ian B. Masters, Helen L. Buntain, John W. Upham

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects.

Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed.

Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways.

Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

LanguageEnglish
Pages575-582
Number of pages8
JournalPediatric Pulmonology
Volume53
Issue number5
DOIs
StatePublished - May 2018

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Bronchiectasis
Bronchitis
Transcriptome
Dimercaprol
Haemophilus influenzae
Inflammation
Interleukin-10
Lung
Bacteria
Gene Expression
Haemophilus influenzae type b
PPAR gamma
Bronchoalveolar Lavage
Infection
Cough
Interleukin-6
Macrophages

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Chen , A. C-H., Pena, O. M., Nel, H. J., Yerkovich, S., Chang, A., J. Baines, K., ... Upham, J. W. (2018). Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. Pediatric Pulmonology, 53(5), 575-582. DOI: 10.1002/ppul.23984
Chen , Alice C-H ; Pena, Olga M. ; Nel, Hendrik J. ; Yerkovich, Stephanie ; Chang, Anne ; J. Baines, Katherine ; Gibson, Peter G. ; Petsky, Helen L. ; J. Pizzutto, Susan ; Hodge, Sandra ; Masters, Ian B. ; Buntain, Helen L. ; Upham, John W./ Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. In: Pediatric Pulmonology. 2018 ; Vol. 53, No. 5. pp. 575-582
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title = "Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles",
abstract = "Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.",
author = "Chen, {Alice C-H} and Pena, {Olga M.} and Nel, {Hendrik J.} and Stephanie Yerkovich and Anne Chang and {J. Baines}, Katherine and Gibson, {Peter G.} and Petsky, {Helen L.} and {J. Pizzutto}, Susan and Sandra Hodge and Masters, {Ian B.} and Buntain, {Helen L.} and Upham, {John W.}",
year = "2018",
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doi = "10.1002/ppul.23984",
language = "English",
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Chen , AC-H, Pena, OM, Nel, HJ, Yerkovich, S, Chang, A, J. Baines, K, Gibson, PG, Petsky, HL, J. Pizzutto, S, Hodge, S, Masters, IB, Buntain, HL & Upham, JW 2018, 'Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles' Pediatric Pulmonology, vol. 53, no. 5, pp. 575-582. DOI: 10.1002/ppul.23984

Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. / Chen , Alice C-H; Pena, Olga M. ; Nel, Hendrik J. ; Yerkovich, Stephanie; Chang, Anne; J. Baines, Katherine ; Gibson, Peter G.; Petsky, Helen L.; J. Pizzutto, Susan; Hodge, Sandra; Masters, Ian B.; Buntain, Helen L. ; Upham, John W.

In: Pediatric Pulmonology, Vol. 53, No. 5, 05.2018, p. 575-582.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles

AU - Chen ,Alice C-H

AU - Pena,Olga M.

AU - Nel,Hendrik J.

AU - Yerkovich,Stephanie

AU - Chang,Anne

AU - J. Baines,Katherine

AU - Gibson,Peter G.

AU - Petsky,Helen L.

AU - J. Pizzutto,Susan

AU - Hodge, Sandra

AU - Masters,Ian B.

AU - Buntain,Helen L.

AU - Upham,John W.

PY - 2018/5

Y1 - 2018/5

N2 - Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

AB - Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

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DO - 10.1002/ppul.23984

M3 - Article

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SP - 575

EP - 582

JO - Pediatric Pulmonology

T2 - Pediatric Pulmonology

JF - Pediatric Pulmonology

SN - 1099-0496

IS - 5

ER -

Chen AC-H, Pena OM, Nel HJ, Yerkovich S, Chang A, J. Baines K et al. Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. Pediatric Pulmonology. 2018 May;53(5):575-582. Available from, DOI: 10.1002/ppul.23984