Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles

Alice C-H Chen , Olga M. Pena, Hendrik J. Nel, Stephanie Yerkovich, Anne Chang, Katherine J. Baines, Peter G. Gibson, Helen L. Petsky, Susan Pizzutto, Sandra Hodge, Ian B. Masters, Helen L. Buntain, John W. Upham

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects.

    Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed.

    Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways.

    Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

    Original languageEnglish
    Pages (from-to)575-582
    Number of pages8
    JournalPediatric Pulmonology
    Volume53
    Issue number5
    DOIs
    Publication statusPublished - May 2018

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    Bronchiectasis
    Bronchitis
    Transcriptome
    Dimercaprol
    Haemophilus influenzae
    Interleukin-10
    Lung
    Inflammation
    Bacteria
    Gene Expression
    Peroxisome Proliferator-Activated Receptors
    Bronchoalveolar Lavage
    Infection
    Cough
    Interleukin-6
    Macrophages

    Cite this

    Chen , A. C-H., Pena, O. M., Nel, H. J., Yerkovich, S., Chang, A., J. Baines, K., ... Upham, J. W. (2018). Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. Pediatric Pulmonology, 53(5), 575-582. https://doi.org/10.1002/ppul.23984
    Chen , Alice C-H ; Pena, Olga M. ; Nel, Hendrik J. ; Yerkovich, Stephanie ; Chang, Anne ; J. Baines, Katherine ; Gibson, Peter G. ; Petsky, Helen L. ; Pizzutto, Susan ; Hodge, Sandra ; Masters, Ian B. ; Buntain, Helen L. ; Upham, John W. / Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. In: Pediatric Pulmonology. 2018 ; Vol. 53, No. 5. pp. 575-582.
    @article{0bdcd79361af4b398df7af27e37ec2a5,
    title = "Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles",
    abstract = "Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.",
    author = "Chen, {Alice C-H} and Pena, {Olga M.} and Nel, {Hendrik J.} and Stephanie Yerkovich and Anne Chang and {J. Baines}, Katherine and Gibson, {Peter G.} and Petsky, {Helen L.} and Susan Pizzutto and Sandra Hodge and Masters, {Ian B.} and Buntain, {Helen L.} and Upham, {John W.}",
    year = "2018",
    month = "5",
    doi = "10.1002/ppul.23984",
    language = "English",
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    Chen , AC-H, Pena, OM, Nel, HJ, Yerkovich, S, Chang, A, J. Baines, K, Gibson, PG, Petsky, HL, Pizzutto, S, Hodge, S, Masters, IB, Buntain, HL & Upham, JW 2018, 'Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles', Pediatric Pulmonology, vol. 53, no. 5, pp. 575-582. https://doi.org/10.1002/ppul.23984

    Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles. / Chen , Alice C-H; Pena, Olga M. ; Nel, Hendrik J. ; Yerkovich, Stephanie; Chang, Anne; J. Baines, Katherine ; Gibson, Peter G.; Petsky, Helen L.; Pizzutto, Susan; Hodge, Sandra; Masters, Ian B.; Buntain, Helen L. ; Upham, John W.

    In: Pediatric Pulmonology, Vol. 53, No. 5, 05.2018, p. 575-582.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Airway cells from protracted bacterial bronchitis and bronchiectasis share similar gene expression profiles

    AU - Chen , Alice C-H

    AU - Pena, Olga M.

    AU - Nel, Hendrik J.

    AU - Yerkovich, Stephanie

    AU - Chang, Anne

    AU - J. Baines, Katherine

    AU - Gibson, Peter G.

    AU - Petsky, Helen L.

    AU - Pizzutto, Susan

    AU - Hodge, Sandra

    AU - Masters, Ian B.

    AU - Buntain, Helen L.

    AU - Upham, John W.

    PY - 2018/5

    Y1 - 2018/5

    N2 - Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

    AB - Aim: Protracted bacterial bronchitis (PBB) is a common cause of prolonged cough in young children, and may be a precursor of bronchiectasis. Bacteria are often present in the lower airways in both PBB and bronchiectasis and may cause persistent infections. However, there is a paucity of information available on the pathogenesis of PBB and the factors associated with persistent bacterial infection and progression to bronchiectasis. This study hypothesised that lung immune cells in recurrent PBB and bronchiectasis differentially express genes related to immune cell dysfunction compared to lung immune cells from control subjects. Method: Cells isolated from bronchoalveolar lavage (adult‐control and PBB BAL cells) were stimulated with nontypeable Haemophilus influenzae (NTHi), and expression of genes involved in various inflammatory pathways was assessed. Result: NTHi induced production of large amounts of IL‐1β, IL‐6, and IL‐8 in adult‐control BAL cells, however BAL cells from PBB airways appeared refractory to NTHi stimulation. BAL cells from PBB and bronchiectasis showed differential expression of several genes relative to control cells, including CCL20, MARCO, CCL24, IL‐10, PPAR‐γ, CD200R, TREM2, RelB. Expression of genes involved in resolution of inflammation and anti‐inflammation response, such as CD200R and IL‐10, was associated with the number of pathogenic bacteria found in the airways. Conclusion: In summary, we have shown that the expression of genes related to macrophage function and resolution of inflammation are similar in PBB and bronchiectasis. Lung immune cell dysfunction in PBB and bronchiectasis may contribute to poor bacterial clearance and prolonged resolution of inflammation.

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    SN - 1099-0496

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