An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri

K WINTER, Geoffrey Isbister, J SEYMOUR, W HODGSON

Research output: Contribution to journalArticleResearchpeer-review

Abstract

We have previously characterised the pharmacological activity of a number of jellyfish venoms with a particular emphasis on the profound cardiovascular effects. It has been suggested that jellyfish venoms are difficult to work with and are sensitive to pH, temperature and chemical changes. The current study aimed to examine the working parameters of the venom of the Australian box jellyfish Chironex fleckeri to enable fractionation and isolation of the toxins with cardiovascular activity. C. fleckeri venom was made up fresh each day and subjected to a number of different environments (i.e. a pH range of 5-9 and a temperature range of 4-30 �C). In addition, the effect of freeze drying and reconstituting the venom was investigated. Venom (50 ?g/kg, i.v.) produced a transient hypertensive response followed by cardiovascular collapse in anaesthetised rats. This biphasic response was not significantly effected by preparation of the venom at a pH of 5, 7 or 9. Similarly, venom (50 ?g/kg, i.v.) did not display a loss of activity when exposed to temperatures of 4, 20 or 30 �C for 1.5 h. However, the cardiovascular activity was abolished by boiling the venom. Freeze drying, and then reconstituting, the venom did not significantly affect its cardiovascular activity. However, repeated freeze drying and reconstituting of extracted venom resulted in a significantly loss of activity. This study provides a more detailed knowledge of the parameters in which C. fleckeri venom can be used and, while supporting some previous studies, contradicts some of the perceived problems of working with the venom. � 2006 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)804-809
Number of pages6
JournalToxicon
Volume49
Issue number6
Publication statusPublished - 2007

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Cubozoa
Venoms
Freeze Drying
Cnidarian Venoms
Drying
Temperature
Fractionation
Transient analysis
Boiling liquids

Cite this

WINTER, K., Isbister, G., SEYMOUR, J., & HODGSON, W. (2007). An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri. Toxicon, 49(6), 804-809.
WINTER, K ; Isbister, Geoffrey ; SEYMOUR, J ; HODGSON, W. / An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri. In: Toxicon. 2007 ; Vol. 49, No. 6. pp. 804-809.
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WINTER, K, Isbister, G, SEYMOUR, J & HODGSON, W 2007, 'An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri', Toxicon, vol. 49, no. 6, pp. 804-809.

An in vivo examination of the stability of venom from the Australian box jellyfish Chironex fleckeri. / WINTER, K; Isbister, Geoffrey; SEYMOUR, J; HODGSON, W.

In: Toxicon, Vol. 49, No. 6, 2007, p. 804-809.

Research output: Contribution to journalArticleResearchpeer-review

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