Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum ?-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants. Copyright � 2009, American Society for Microbiology. All Rights Reserved.
|Number of pages||5|
|Journal||Antimicrobial Agents and Chemotherapy|
|Publication status||Published - 2009|