Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis

Patiyan Andersson, Tegan Harris, Steven Tong, Philip Giffard

Research output: Contribution to journalArticlepeer-review

Abstract

Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum ?-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants. Copyright � 2009, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)2679-2683
Number of pages5
JournalAntimicrobial Agents and Chemotherapy
Volume53
Issue number6
Publication statusPublished - 2009

Fingerprint

Dive into the research topics of 'Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis'. Together they form a unique fingerprint.

Cite this