Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis

Patiyan Andersson, Tegan Harris, Steven Tong, Philip Giffard

    Research output: Contribution to journalArticle

    Abstract

    Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum ?-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants. Copyright � 2009, American Society for Microbiology. All Rights Reserved.
    Original languageEnglish
    Pages (from-to)2679-2683
    Number of pages5
    JournalAntimicrobial Agents and Chemotherapy
    Volume53
    Issue number6
    Publication statusPublished - 2009

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