Klebsiella pneumoniae isolates frequently contain complex mixtures of blaSHV alleles. A high-resolution melting-based method for interrogating the extended-spectrum activity conferring codon 238 and 240 polymorphisms was developed. This detects minority extended-spectrum ?-lactamase-encoding alleles, allows estimation of allele ratios, and discriminates between single and double mutants. Copyright � 2009, American Society for Microbiology. All Rights Reserved.
|Number of pages||5|
|Journal||Antimicrobial Agents and Chemotherapy|
|Publication status||Published - 2009|
Andersson, P., Harris, T., Tong, S., & Giffard, P. (2009). Analysis of blaSHV codon 238 and 240 allele mixtures using Sybr green high-resolution melting analysis. Antimicrobial Agents and Chemotherapy, 53(6), 2679-2683.