@article{4cb38b4f130c4fb5b2ce977d46e6b001,
title = "Antibody Responses to Sarcoptes scabiei Apolipoprotein in a Porcine Model: Relevance to Immunodiagnosis of Recent Infection",
abstract = "No commercial immunodiagnostic tests for human scabies are currently available, and existing animal tests are not sufficiently sensitive. The recombinant Sarcoptes scabiei apolipoprotein antigen Sar s 14.3 is a promising immunodiagnostic, eliciting high levels of IgE and IgG in infected people. Limited data are available regarding the temporal development of antibodies to Sar s 14.3, an issue of relevance in terms of immunodiagnosis. We utilised a porcine model to prospectively compare specific antibody responses to a primary infestation by ELISA, to Sar s 14.3 and to S. scabiei whole mite antigen extract (WMA). Differences in the antibody profile between antigens were apparent, with Sar s 14.3 responses detected earlier, and declining significantly after peak infestation compared to WMA. Both antigens resulted in >90% diagnostic sensitivity from weeks 8-16 post infestation. These data provide important information on the temporal development of humoral immune responses in scabies and further supports the development of recombinant antigen based immunodiagnostic tests for recent scabies infestations. ",
keywords = "apolipoprotein, dexamethasone, immunoglobulin A, immunoglobulin G, immunoglobulin G1, immunoglobulin G2, immunoglobulin M, parasite antigen, unclassified drug, whole mite antigen, antigen, glucocorticoid, immunoglobulin E, recombinant protein, animal experiment, animal model, antibody response, antigen detection, article, controlled study, enzyme linked immunosorbent assay, female, humoral immunity, immunoglobulin blood level, mite infestation, nonhuman, nucleotide sequence, Sarcoptes scabiei, scabies, sensitivity analysis, serodiagnosis, animal, biosynthesis, blood, drug effects, ear, human, immunology, parasitology, skin, swine, Swine Diseases, time, veterinary, Acari, Animalia, Sus, Animals, Antigens, Dexamethasone, Ear, Enzyme-Linked Immunosorbent Assay, Female, Glucocorticoids, Humans, Immunity, Humoral, Immunoglobulin E, Immunoglobulin G, Recombinant Proteins, Scabies, Skin, Swine, Time Factors",
author = "Melanie Rampton and Walton, {Shelley Faye} and Deborah Holt and Cielo Pasay and Andrew Kelly and Bart Currie and James McCarthy and Kate Mounsey",
year = "2013",
doi = "10.1371/journal.pone.0065354",
language = "English",
volume = "8",
pages = "1--10",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science (PLoS)",
number = "6",
}