Antimicrobial resistance in urine and skin isolates in Timor-Leste

Ian Marr, Nevio Sarmento, Matt O'Brien, Karl Lee, Celia Gusmao, Gloria de Castro, Sonja Janson, Steven Y.C. Tong, Rob W. Baird, Joshua R. Francis

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. 

Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. 

Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. 

Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

LanguageEnglish
Pages135-138
Number of pages4
JournalJournal of Global Antimicrobial Resistance
Volume13
DOIs
StatePublished - Jun 2018

Fingerprint

Urine
Skin
Staphylococcus aureus
Phenotype
Southeastern Asia
Methicillin Resistance
Carbapenems
Indonesia
Ceftriaxone
Enterobacteriaceae
beta-Lactamases
Methicillin-Resistant Staphylococcus aureus
Economics
Timor-Leste
Guidelines
Bacteria

Cite this

Marr, I., Sarmento, N., O'Brien, M., Lee, K., Gusmao, C., de Castro, G., ... Francis, J. R. (2018). Antimicrobial resistance in urine and skin isolates in Timor-Leste. Journal of Global Antimicrobial Resistance, 13, 135-138. DOI: 10.1016/j.jgar.2017.12.010
Marr, Ian ; Sarmento, Nevio ; O'Brien, Matt ; Lee, Karl ; Gusmao, Celia ; de Castro, Gloria ; Janson, Sonja ; Tong, Steven Y.C. ; Baird, Rob W. ; Francis, Joshua R./ Antimicrobial resistance in urine and skin isolates in Timor-Leste. In: Journal of Global Antimicrobial Resistance. 2018 ; Vol. 13. pp. 135-138
@article{0a8faba33c6a418bbaaa3cee72cdfc0d,
title = "Antimicrobial resistance in urine and skin isolates in Timor-Leste",
abstract = "Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK{\circledR}2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35{\%} were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11{\%} were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.",
keywords = "Antimicrobial resistance, Bacteria, ESBL, Extended-spectrum β-lactamase, MRSA, Timor-Leste",
author = "Ian Marr and Nevio Sarmento and Matt O'Brien and Karl Lee and Celia Gusmao and {de Castro}, Gloria and Sonja Janson and Tong, {Steven Y.C.} and Baird, {Rob W.} and Francis, {Joshua R.}",
year = "2018",
month = "6",
doi = "10.1016/j.jgar.2017.12.010",
language = "English",
volume = "13",
pages = "135--138",
journal = "Journal of Global Antimicrobial Resistance",
issn = "2213-7165",

}

Marr, I, Sarmento, N, O'Brien, M, Lee, K, Gusmao, C, de Castro, G, Janson, S, Tong, SYC, Baird, RW & Francis, JR 2018, 'Antimicrobial resistance in urine and skin isolates in Timor-Leste' Journal of Global Antimicrobial Resistance, vol. 13, pp. 135-138. DOI: 10.1016/j.jgar.2017.12.010

Antimicrobial resistance in urine and skin isolates in Timor-Leste. / Marr, Ian; Sarmento, Nevio; O'Brien, Matt; Lee, Karl; Gusmao, Celia; de Castro, Gloria; Janson, Sonja; Tong, Steven Y.C.; Baird, Rob W.; Francis, Joshua R.

In: Journal of Global Antimicrobial Resistance, Vol. 13, 06.2018, p. 135-138.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antimicrobial resistance in urine and skin isolates in Timor-Leste

AU - Marr,Ian

AU - Sarmento,Nevio

AU - O'Brien,Matt

AU - Lee,Karl

AU - Gusmao,Celia

AU - de Castro,Gloria

AU - Janson,Sonja

AU - Tong,Steven Y.C.

AU - Baird,Rob W.

AU - Francis,Joshua R.

PY - 2018/6

Y1 - 2018/6

N2 - Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

AB - Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

KW - Antimicrobial resistance

KW - Bacteria

KW - ESBL

KW - Extended-spectrum β-lactamase

KW - MRSA

KW - Timor-Leste

UR - http://www.scopus.com/inward/record.url?scp=85046117156&partnerID=8YFLogxK

U2 - 10.1016/j.jgar.2017.12.010

DO - 10.1016/j.jgar.2017.12.010

M3 - Article

VL - 13

SP - 135

EP - 138

JO - Journal of Global Antimicrobial Resistance

T2 - Journal of Global Antimicrobial Resistance

JF - Journal of Global Antimicrobial Resistance

SN - 2213-7165

ER -

Marr I, Sarmento N, O'Brien M, Lee K, Gusmao C, de Castro G et al. Antimicrobial resistance in urine and skin isolates in Timor-Leste. Journal of Global Antimicrobial Resistance. 2018 Jun;13:135-138. Available from, DOI: 10.1016/j.jgar.2017.12.010