Antimicrobial resistance in urine and skin isolates in Timor-Leste

Ian Marr, Nevio Sarmento, Matt O'Brien, Karl Lee, Celia Gusmao, Gloria de Castro, Sonja Janson, Steven Y.C. Tong, Rob W. Baird, Joshua R. Francis

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. 

    Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. 

    Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. 

    Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

    LanguageEnglish
    Pages135-138
    Number of pages4
    JournalJournal of Global Antimicrobial Resistance
    Volume13
    DOIs
    Publication statusPublished - Jun 2018

    Fingerprint

    Urine
    Skin
    Staphylococcus aureus
    Phenotype
    Southeastern Asia
    Methicillin Resistance
    Carbapenems
    Indonesia
    Ceftriaxone
    Enterobacteriaceae
    beta-Lactamases
    Methicillin-Resistant Staphylococcus aureus
    Economics
    Timor-Leste
    Guidelines
    Bacteria

    Cite this

    Marr, I., Sarmento, N., O'Brien, M., Lee, K., Gusmao, C., de Castro, G., ... Francis, J. R. (2018). Antimicrobial resistance in urine and skin isolates in Timor-Leste. Journal of Global Antimicrobial Resistance, 13, 135-138. https://doi.org/10.1016/j.jgar.2017.12.010
    Marr, Ian ; Sarmento, Nevio ; O'Brien, Matt ; Lee, Karl ; Gusmao, Celia ; de Castro, Gloria ; Janson, Sonja ; Tong, Steven Y.C. ; Baird, Rob W. ; Francis, Joshua R. / Antimicrobial resistance in urine and skin isolates in Timor-Leste. In: Journal of Global Antimicrobial Resistance. 2018 ; Vol. 13. pp. 135-138.
    @article{0a8faba33c6a418bbaaa3cee72cdfc0d,
    title = "Antimicrobial resistance in urine and skin isolates in Timor-Leste",
    abstract = "Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK{\circledR}2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35{\%} were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11{\%} were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.",
    keywords = "Antimicrobial resistance, Bacteria, ESBL, Extended-spectrum β-lactamase, MRSA, Timor-Leste",
    author = "Ian Marr and Nevio Sarmento and Matt O'Brien and Karl Lee and Celia Gusmao and {de Castro}, Gloria and Sonja Janson and Tong, {Steven Y.C.} and Baird, {Rob W.} and Francis, {Joshua R.}",
    year = "2018",
    month = "6",
    doi = "10.1016/j.jgar.2017.12.010",
    language = "English",
    volume = "13",
    pages = "135--138",
    journal = "Journal of Global Antimicrobial Resistance",
    issn = "2213-7165",
    publisher = "Elsevier",

    }

    Marr, I, Sarmento, N, O'Brien, M, Lee, K, Gusmao, C, de Castro, G, Janson, S, Tong, SYC, Baird, RW & Francis, JR 2018, 'Antimicrobial resistance in urine and skin isolates in Timor-Leste', Journal of Global Antimicrobial Resistance, vol. 13, pp. 135-138. https://doi.org/10.1016/j.jgar.2017.12.010

    Antimicrobial resistance in urine and skin isolates in Timor-Leste. / Marr, Ian; Sarmento, Nevio; O'Brien, Matt; Lee, Karl; Gusmao, Celia; de Castro, Gloria; Janson, Sonja; Tong, Steven Y.C.; Baird, Rob W.; Francis, Joshua R.

    In: Journal of Global Antimicrobial Resistance, Vol. 13, 06.2018, p. 135-138.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Antimicrobial resistance in urine and skin isolates in Timor-Leste

    AU - Marr, Ian

    AU - Sarmento, Nevio

    AU - O'Brien, Matt

    AU - Lee, Karl

    AU - Gusmao, Celia

    AU - de Castro, Gloria

    AU - Janson, Sonja

    AU - Tong, Steven Y.C.

    AU - Baird, Rob W.

    AU - Francis, Joshua R.

    PY - 2018/6

    Y1 - 2018/6

    N2 - Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

    AB - Objectives: High rates of antimicrobial resistance (AMR) are seen throughout Southeast Asia. However, limited AMR data exist for Timor-Leste, which is situated on the south-eastern portion of the Malay Archipelago. The purpose of this study was to identify AMR in bacteria isolated from urine and skin swabs from patients in Dili, the capital of Timor-Leste. Methods: Urine and skin swabs were collected from symptomatic patients in Timor-Leste and were processed for bacterial culture. Isolates were processed in Australia using a VITEK®2 system for bacterial identification and to determine antimicrobial susceptibility according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Results: A total of 154 urine isolates and 57 skin isolates were analysed. Of the Enterobacteriaceae, 35% were resistant to ceftriaxone with an extended-spectrum β-lactamase (ESBL)-producing phenotype. Carbapenem resistance was not observed in any of the Gram-negative isolates. Of the Staphylococcus aureus isolates, 11% were of the community-associated methicillin-resistant S. aureus (CA-MRSA) phenotype. Conclusions: A moderately high proportion of Gram-negative urine isolates in Timor-Leste demonstrate phenotypic ESBL production, and a relatively low proportion of S. aureus isolates were methicillin-resistant. Improved understanding of AMR rates in Timor-Leste can help guide antimicrobial prescribing and inform antimicrobial stewardship strategies.

    KW - Antimicrobial resistance

    KW - Bacteria

    KW - ESBL

    KW - Extended-spectrum β-lactamase

    KW - MRSA

    KW - Timor-Leste

    UR - http://www.scopus.com/inward/record.url?scp=85046117156&partnerID=8YFLogxK

    U2 - 10.1016/j.jgar.2017.12.010

    DO - 10.1016/j.jgar.2017.12.010

    M3 - Article

    VL - 13

    SP - 135

    EP - 138

    JO - Journal of Global Antimicrobial Resistance

    T2 - Journal of Global Antimicrobial Resistance

    JF - Journal of Global Antimicrobial Resistance

    SN - 2213-7165

    ER -