Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species

Bridget E. Barber, Matthew J. Grigg, Kim Piera, Fiona H. Amante, Timothy William, Michelle J. Boyle, Gabriela Minigo, Arjen M. Dondorp, James S. McCarthy, Nicholas M. Anstey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. 

Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. 

Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. 

Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

Original languageEnglish
Pages (from-to)1435-1443
Number of pages9
JournalThe Journal of infectious diseases
Volume220
Issue number9
DOIs
Publication statusPublished - 1 Nov 2019

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Falciparum Malaria
Vivax Malaria
Immunoglobulin M
Anemia
Immunoglobulin G
Plasmodium vivax
Antibodies
Malaria
Hemoglobins
Plasmodium falciparum
Volunteers
Erythrocytes
Plasmodium
Parasitemia
Phagocytosis
Fever

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@article{f1f4761f405c44b1a3f1dfed3201d9bb,
title = "Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species",
abstract = "Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.",
keywords = "Plasmodium falciparum, Plasmodium knowlesi, Plasmodium malariae, Plasmodium vivax, anemia, malaria, phosphatidylserine antibodies",
author = "Barber, {Bridget E.} and Grigg, {Matthew J.} and Kim Piera and Amante, {Fiona H.} and Timothy William and Boyle, {Michelle J.} and Gabriela Minigo and Dondorp, {Arjen M.} and McCarthy, {James S.} and Anstey, {Nicholas M.}",
year = "2019",
month = "11",
day = "1",
doi = "10.1093/infdis/jiz334",
language = "English",
volume = "220",
pages = "1435--1443",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "9",

}

Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species. / Barber, Bridget E.; Grigg, Matthew J.; Piera, Kim; Amante, Fiona H.; William, Timothy; Boyle, Michelle J.; Minigo, Gabriela; Dondorp, Arjen M.; McCarthy, James S.; Anstey, Nicholas M.

In: The Journal of infectious diseases, Vol. 220, No. 9, 01.11.2019, p. 1435-1443.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species

AU - Barber, Bridget E.

AU - Grigg, Matthew J.

AU - Piera, Kim

AU - Amante, Fiona H.

AU - William, Timothy

AU - Boyle, Michelle J.

AU - Minigo, Gabriela

AU - Dondorp, Arjen M.

AU - McCarthy, James S.

AU - Anstey, Nicholas M.

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

AB - Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

KW - Plasmodium falciparum

KW - Plasmodium knowlesi

KW - Plasmodium malariae

KW - Plasmodium vivax

KW - anemia

KW - malaria

KW - phosphatidylserine antibodies

UR - http://www.scopus.com/inward/record.url?scp=85072716637&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiz334

DO - 10.1093/infdis/jiz334

M3 - Article

VL - 220

SP - 1435

EP - 1443

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 9

ER -