Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species

Bridget E. Barber, Matthew J. Grigg, Kim Piera, Fiona H. Amante, Timothy William, Michelle J. Boyle, Gabriela Minigo, Arjen M. Dondorp, James S. McCarthy, Nicholas M. Anstey

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. 

    Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. 

    Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. 

    Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

    Original languageEnglish
    Pages (from-to)1435-1443
    Number of pages9
    JournalThe Journal of infectious diseases
    Volume220
    Issue number9
    DOIs
    Publication statusPublished - 1 Nov 2019

    Fingerprint

    Falciparum Malaria
    Vivax Malaria
    Immunoglobulin M
    Anemia
    Immunoglobulin G
    Plasmodium vivax
    Antibodies
    Malaria
    Hemoglobins
    Plasmodium falciparum
    Volunteers
    Erythrocytes
    Plasmodium
    Parasitemia
    Phagocytosis
    Fever

    Cite this

    @article{f1f4761f405c44b1a3f1dfed3201d9bb,
    title = "Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species",
    abstract = "Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.",
    keywords = "Plasmodium falciparum, Plasmodium knowlesi, Plasmodium malariae, Plasmodium vivax, anemia, malaria, phosphatidylserine antibodies",
    author = "Barber, {Bridget E.} and Grigg, {Matthew J.} and Kim Piera and Amante, {Fiona H.} and Timothy William and Boyle, {Michelle J.} and Gabriela Minigo and Dondorp, {Arjen M.} and McCarthy, {James S.} and Anstey, {Nicholas M.}",
    year = "2019",
    month = "11",
    day = "1",
    doi = "10.1093/infdis/jiz334",
    language = "English",
    volume = "220",
    pages = "1435--1443",
    journal = "Journal of Infectious Diseases",
    issn = "0022-1899",
    publisher = "Oxford University Press",
    number = "9",

    }

    Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species. / Barber, Bridget E.; Grigg, Matthew J.; Piera, Kim; Amante, Fiona H.; William, Timothy; Boyle, Michelle J.; Minigo, Gabriela; Dondorp, Arjen M.; McCarthy, James S.; Anstey, Nicholas M.

    In: The Journal of infectious diseases, Vol. 220, No. 9, 01.11.2019, p. 1435-1443.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Antiphosphatidylserine Immunoglobulin M and Immunoglobulin G Antibodies Are Higher in Vivax Than Falciparum Malaria, and Associated With Early Anemia in Both Species

    AU - Barber, Bridget E.

    AU - Grigg, Matthew J.

    AU - Piera, Kim

    AU - Amante, Fiona H.

    AU - William, Timothy

    AU - Boyle, Michelle J.

    AU - Minigo, Gabriela

    AU - Dondorp, Arjen M.

    AU - McCarthy, James S.

    AU - Anstey, Nicholas M.

    PY - 2019/11/1

    Y1 - 2019/11/1

    N2 - Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

    AB - Background: Anemia is a major complication of vivax malaria. Antiphosphatidylserine (PS) antibodies generated during falciparum malaria mediate phagocytosis of uninfected red blood cells that expose PS and have been linked to late malarial anemia. However, their role in anemia from non-falciparum Plasmodium species is not known, nor their role in early anemia from falciparum malaria. Methods: We measured PS immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies in Malaysian patients with vivax, falciparum, knowlesi, and malariae malaria, and in healthy controls, and correlated antibody titres with hemoglobin. PS antibodies were also measured in volunteers experimentally infected with Plasmodium vivax and Plasmodium falciparum. Results: PS IgM and IgG antibodies were elevated in patients with vivax, falciparum, knowlesi, and malariae malaria (P < .0001 for all comparisons with controls) and were highest in vivax malaria. In vivax and falciparum malaria, PS IgM and IgG on admission correlated inversely with admission and nadir hemoglobin, controlling for parasitemia and fever duration. PS IgM and IgG were also increased in volunteers infected with blood-stage P. vivax and P. falciparum, and were higher in P. vivax infection. Conclusions: PS antibodies are higher in vivax than falciparum malaria, correlate inversely with hemoglobin, and may contribute to the early loss of uninfected red blood cells found in malarial anemia from both species.

    KW - Plasmodium falciparum

    KW - Plasmodium knowlesi

    KW - Plasmodium malariae

    KW - Plasmodium vivax

    KW - anemia

    KW - malaria

    KW - phosphatidylserine antibodies

    UR - http://www.scopus.com/inward/record.url?scp=85072716637&partnerID=8YFLogxK

    U2 - 10.1093/infdis/jiz334

    DO - 10.1093/infdis/jiz334

    M3 - Article

    VL - 220

    SP - 1435

    EP - 1443

    JO - Journal of Infectious Diseases

    JF - Journal of Infectious Diseases

    SN - 0022-1899

    IS - 9

    ER -