Application of a Method for Obtaining Lymph from Anuran Amphibians

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    Methods for obtaining plasma from anurans are unsatisfactory in that they are inadequately described, are difficult to perform, or are lethal to the subject. A lymph extraction technique involving temporary ligation is described that enables sufficient fluid to be obtained for analysis from frogs greater than 20 g. Lymph was more easily obtained from Bufo marinus than from the austral-hylids Litoria caerulea and Cyclorana australis. Lymph accumulation was faster in hydrated frogs or those with full bladders, whereas it was relatively slow in animals that had been dormant or that were dehydrated. Differences between the osmolality of lymph and blood-plasma were variable but slight; presumably the relatively high endothelial permeability of amphibians facilitates significant fluid exchange. Copyright � 2009 Society for the Study of Amphibians and Reptiles.
    Original languageEnglish
    Pages (from-to)148-153
    Number of pages6
    JournalJournal of Herpetology
    Issue number1
    Publication statusPublished - 2009


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