Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry

Anna Padovan, Karen Gibb, Denis Persley

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The identity of phytoplasmas detected in strawberry plants with green petal (SGP) and lethal yellows (SLY) diseases was determined by RFLP analysis of the 16S rRNA gene and adjacent spacer region (SR). RFLP and sequence comparisons indicated that the phytoplasmas associated with SGP and SLY were indistinguishable and were most closely related to `Candidatus Phytoplasma australiense', the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases. This taxon lies within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify only members of the AY strain cluster, amplified a DNA product from the SGP and SLY phytoplasmas. Primers deduced from the 16S rRNA/SR of P. australiense that amplify only members of this taxon amplified rDNA sequences from the SGP and SLY phytoplasmas. Primers that selectively amplify members of the faba bean phyllody (FBP) phytoplasma group, the most commonly occurring phytoplasma group in Australia, did not amplify rDNA from the SGP and SLY phytoplasmas.
    Original languageEnglish
    Pages (from-to)362-369
    Number of pages8
    JournalPlant Pathology
    Volume49
    Issue number3
    DOIs
    Publication statusPublished - Jun 2000

    Fingerprint

    Phytoplasma
    Fragaria
    lethal genes
    corolla
    strawberries
    Faba bean phyllody phytoplasma
    Ribosomal DNA
    Restriction Fragment Length Polymorphisms
    restriction fragment length polymorphism
    Phormium
    aster yellows
    ribosomal RNA
    Viridiplantae
    Carica
    Vicia faba
    foliar diseases
    papayas
    dieback
    rRNA Genes
    genes

    Cite this

    @article{8052a626294e4b11b753c764a37a3c17,
    title = "Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry",
    abstract = "The identity of phytoplasmas detected in strawberry plants with green petal (SGP) and lethal yellows (SLY) diseases was determined by RFLP analysis of the 16S rRNA gene and adjacent spacer region (SR). RFLP and sequence comparisons indicated that the phytoplasmas associated with SGP and SLY were indistinguishable and were most closely related to `Candidatus Phytoplasma australiense', the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases. This taxon lies within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify only members of the AY strain cluster, amplified a DNA product from the SGP and SLY phytoplasmas. Primers deduced from the 16S rRNA/SR of P. australiense that amplify only members of this taxon amplified rDNA sequences from the SGP and SLY phytoplasmas. Primers that selectively amplify members of the faba bean phyllody (FBP) phytoplasma group, the most commonly occurring phytoplasma group in Australia, did not amplify rDNA from the SGP and SLY phytoplasmas.",
    keywords = "16SrRNA, heterogeneity, phytoplasma, strawberry",
    author = "Anna Padovan and Karen Gibb and Denis Persley",
    year = "2000",
    month = "6",
    doi = "10.1046/j.1365-3059.2000.00461.x",
    language = "English",
    volume = "49",
    pages = "362--369",
    journal = "Plant Pathology",
    issn = "0032-0862",
    publisher = "Global Science Books",
    number = "3",

    }

    Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry. / Padovan, Anna; Gibb, Karen; Persley, Denis.

    In: Plant Pathology, Vol. 49, No. 3, 06.2000, p. 362-369.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Association of 'Candidatus Phytoplasma australiense' with green petal and lethal yellows diseases in strawberry

    AU - Padovan, Anna

    AU - Gibb, Karen

    AU - Persley, Denis

    PY - 2000/6

    Y1 - 2000/6

    N2 - The identity of phytoplasmas detected in strawberry plants with green petal (SGP) and lethal yellows (SLY) diseases was determined by RFLP analysis of the 16S rRNA gene and adjacent spacer region (SR). RFLP and sequence comparisons indicated that the phytoplasmas associated with SGP and SLY were indistinguishable and were most closely related to `Candidatus Phytoplasma australiense', the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases. This taxon lies within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify only members of the AY strain cluster, amplified a DNA product from the SGP and SLY phytoplasmas. Primers deduced from the 16S rRNA/SR of P. australiense that amplify only members of this taxon amplified rDNA sequences from the SGP and SLY phytoplasmas. Primers that selectively amplify members of the faba bean phyllody (FBP) phytoplasma group, the most commonly occurring phytoplasma group in Australia, did not amplify rDNA from the SGP and SLY phytoplasmas.

    AB - The identity of phytoplasmas detected in strawberry plants with green petal (SGP) and lethal yellows (SLY) diseases was determined by RFLP analysis of the 16S rRNA gene and adjacent spacer region (SR). RFLP and sequence comparisons indicated that the phytoplasmas associated with SGP and SLY were indistinguishable and were most closely related to `Candidatus Phytoplasma australiense', the phytoplasma associated with Australian grapevine yellows, papaya dieback and Phormium yellow leaf diseases. This taxon lies within the aster yellows strain cluster. Primers based on the phytoplasma tuf gene, which amplify only members of the AY strain cluster, amplified a DNA product from the SGP and SLY phytoplasmas. Primers deduced from the 16S rRNA/SR of P. australiense that amplify only members of this taxon amplified rDNA sequences from the SGP and SLY phytoplasmas. Primers that selectively amplify members of the faba bean phyllody (FBP) phytoplasma group, the most commonly occurring phytoplasma group in Australia, did not amplify rDNA from the SGP and SLY phytoplasmas.

    KW - 16SrRNA

    KW - heterogeneity

    KW - phytoplasma

    KW - strawberry

    UR - http://www.scopus.com/inward/record.url?scp=0034125998&partnerID=8YFLogxK

    U2 - 10.1046/j.1365-3059.2000.00461.x

    DO - 10.1046/j.1365-3059.2000.00461.x

    M3 - Article

    VL - 49

    SP - 362

    EP - 369

    JO - Plant Pathology

    JF - Plant Pathology

    SN - 0032-0862

    IS - 3

    ER -