Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera

R TOWERS, M BOLM, Bart Currie, G CHHATWAL, Peter Fagan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Acute rheumatic fever (ARF) is an autoimmune sequela of group A streptococcal infection mostly affecting school-aged children. Recurrent episodes of ARF can result in the development of rheumatic heart disease (RHD). One in 40 indigenous Australians in the Northern Territory is affected by RHD. This disease mostly impacts young people; 45% of those who require heart valve surgery in Australia due to RHD are younger than 25 years old. ARF is characterized by autoimmune attack of the heart; therefore, the presence of the autoantibodies involved could potentially be used to diagnose ARF. To this end, a human heart cDNA library was screened with serum from a patient with ARF, and 12 autoreactive human heart antigens were identified. They include five different IgG heavy chains and a range of tissue-specific cell-signaling proteins, species of which have been implicated in other autoimmune diseases. Preliminary ELISA results show that ARF patients have significantly higher levels of antibodies recognizing the cardiac autoantigens than controls. These antigens are promising candidates for the development of a serological assay for the diagnosis of ARF. The nature of the proteins identified has exciting implications for future research into the pathogenesis of ARF. � 2009 New York Academy of Sciences.
Original languageEnglish
Pages (from-to)83-91
Number of pages9
JournalAnnals of the New York Academy of Sciences
Volume1173
Issue numberContemporary Challenges in Autoimmunity
Publication statusPublished - 2009

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Rheumatic Fever
Autoantigens
Gene Library
Screening
Serum
Rheumatic Heart Disease
Cell signaling
Antigens
Northern Territory
Streptococcal Infections
Fever
Heart Valves
Autoantibodies
Surgery
Assays
Proteins
Autoimmune Diseases
Thoracic Surgery
Immunoglobulin G
Tissue

Cite this

TOWERS, R., BOLM, M., Currie, B., CHHATWAL, G., & Fagan, P. (2009). Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera. Annals of the New York Academy of Sciences, 1173(Contemporary Challenges in Autoimmunity), 83-91.
TOWERS, R ; BOLM, M ; Currie, Bart ; CHHATWAL, G ; Fagan, Peter. / Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera. In: Annals of the New York Academy of Sciences. 2009 ; Vol. 1173, No. Contemporary Challenges in Autoimmunity. pp. 83-91.
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TOWERS, R, BOLM, M, Currie, B, CHHATWAL, G & Fagan, P 2009, 'Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera', Annals of the New York Academy of Sciences, vol. 1173, no. Contemporary Challenges in Autoimmunity, pp. 83-91.

Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera. / TOWERS, R; BOLM, M; Currie, Bart; CHHATWAL, G; Fagan, Peter.

In: Annals of the New York Academy of Sciences, Vol. 1173, No. Contemporary Challenges in Autoimmunity, 2009, p. 83-91.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera

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AU - BOLM, M

AU - Currie, Bart

AU - CHHATWAL, G

AU - Fagan, Peter

PY - 2009

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N2 - Acute rheumatic fever (ARF) is an autoimmune sequela of group A streptococcal infection mostly affecting school-aged children. Recurrent episodes of ARF can result in the development of rheumatic heart disease (RHD). One in 40 indigenous Australians in the Northern Territory is affected by RHD. This disease mostly impacts young people; 45% of those who require heart valve surgery in Australia due to RHD are younger than 25 years old. ARF is characterized by autoimmune attack of the heart; therefore, the presence of the autoantibodies involved could potentially be used to diagnose ARF. To this end, a human heart cDNA library was screened with serum from a patient with ARF, and 12 autoreactive human heart antigens were identified. They include five different IgG heavy chains and a range of tissue-specific cell-signaling proteins, species of which have been implicated in other autoimmune diseases. Preliminary ELISA results show that ARF patients have significantly higher levels of antibodies recognizing the cardiac autoantigens than controls. These antigens are promising candidates for the development of a serological assay for the diagnosis of ARF. The nature of the proteins identified has exciting implications for future research into the pathogenesis of ARF. � 2009 New York Academy of Sciences.

AB - Acute rheumatic fever (ARF) is an autoimmune sequela of group A streptococcal infection mostly affecting school-aged children. Recurrent episodes of ARF can result in the development of rheumatic heart disease (RHD). One in 40 indigenous Australians in the Northern Territory is affected by RHD. This disease mostly impacts young people; 45% of those who require heart valve surgery in Australia due to RHD are younger than 25 years old. ARF is characterized by autoimmune attack of the heart; therefore, the presence of the autoantibodies involved could potentially be used to diagnose ARF. To this end, a human heart cDNA library was screened with serum from a patient with ARF, and 12 autoreactive human heart antigens were identified. They include five different IgG heavy chains and a range of tissue-specific cell-signaling proteins, species of which have been implicated in other autoimmune diseases. Preliminary ELISA results show that ARF patients have significantly higher levels of antibodies recognizing the cardiac autoantigens than controls. These antigens are promising candidates for the development of a serological assay for the diagnosis of ARF. The nature of the proteins identified has exciting implications for future research into the pathogenesis of ARF. � 2009 New York Academy of Sciences.

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KW - vitamin d receptor interacting protein

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KW - autoimmunity

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KW - Acute Disease

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KW - Autoantigens

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KW - Myocardium

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TOWERS R, BOLM M, Currie B, CHHATWAL G, Fagan P. Autoantigens Identified by Screening a Human Heart cDNA Library with Acute Rheumatic Fever Sera. Annals of the New York Academy of Sciences. 2009;1173(Contemporary Challenges in Autoimmunity):83-91.