B Part of It study

a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia

Mark McMillan, Luke Walters, Turra Mark, Andrew Lawrence, Lex E.X. Leong, Thomas Sullivan, Geraint B. Rogers, Ross M. Andrews, Helen S. Marshall

Research output: Contribution to journalArticleResearchpeer-review

1 Downloads (Pure)

Abstract

Objectives: N. meningitidis carriage in Australia is poorly understood. This study aimed to estimate prevalence and risk factors for carriage of N. meningitidis in South Australian university students. We also sought to identify whether delayed freezing of oropharyngeal samples altered PCR positivity, cycle threshold, or culture positivity.

Methods: Oropharyngeal swabs were taken from first year university students and repeated after 3 months, with risk factor questionnaires completed at both visits. Specimens were subjected to real-time PCR screening for the presence of specific meningococcal DNA.

Results: The study enrolled 421 individuals, 259 returned at 3 months. At baseline, 56% of participants were female and 1.9% smokers. Carriage of N. meningitidis at baseline was 6.2% (95% CI, [4.2%, 8.9%]). Visiting a bar more than once a week (OR 9.07; [2.44, 33.72]) and intimate kissing (OR 4.37; [1.45, 13.14]) were associated with increased carriage. After imputing missing data, the point estimate for carriage at 3 months was 8.6% compared to 6.2% at baseline (OR 1.42; 0.91 to 2.20). Recovery of N. meningitidis on selective agar was significantly reduced in cryovials frozen at 48 hours compared to 6 hours (24/26, 92.3% vs. 14/26, 53.9%, p = 0.002).

Conclusion: Attending bars and engaging in intimate kissing is associated with oropharyngeal carriage in South Australian university students. Adolescent meningococcal vaccine programs should be implemented at school, prior to increased attendance at bars, intimate contact, and carriage acquisition. Delaying freezing of oropharyngeal specimens longer than 16 hours reduces yield of N. meningitidis by culture but not PCR detection.

Original languageEnglish
Pages (from-to)987-994
Number of pages8
JournalHuman Vaccines and Immunotherapeutics
Volume15
Issue number4
Early online date4 Jan 2019
DOIs
Publication statusPublished - 3 Apr 2019

Fingerprint

South Australia
Neisseria meningitidis
Longitudinal Studies
Students
Freezing
Meningococcal Vaccines
Polymerase Chain Reaction
Agar
Real-Time Polymerase Chain Reaction
DNA

Cite this

McMillan, Mark ; Walters, Luke ; Mark, Turra ; Lawrence, Andrew ; Leong, Lex E.X. ; Sullivan, Thomas ; Rogers, Geraint B. ; Andrews, Ross M. ; Marshall, Helen S. / B Part of It study : a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia. In: Human Vaccines and Immunotherapeutics. 2019 ; Vol. 15, No. 4. pp. 987-994.
@article{18b45c62457e41998692d2882c997018,
title = "B Part of It study: a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia",
abstract = "Objectives: N. meningitidis carriage in Australia is poorly understood. This study aimed to estimate prevalence and risk factors for carriage of N. meningitidis in South Australian university students. We also sought to identify whether delayed freezing of oropharyngeal samples altered PCR positivity, cycle threshold, or culture positivity. Methods: Oropharyngeal swabs were taken from first year university students and repeated after 3 months, with risk factor questionnaires completed at both visits. Specimens were subjected to real-time PCR screening for the presence of specific meningococcal DNA. Results: The study enrolled 421 individuals, 259 returned at 3 months. At baseline, 56{\%} of participants were female and 1.9{\%} smokers. Carriage of N. meningitidis at baseline was 6.2{\%} (95{\%} CI, [4.2{\%}, 8.9{\%}]). Visiting a bar more than once a week (OR 9.07; [2.44, 33.72]) and intimate kissing (OR 4.37; [1.45, 13.14]) were associated with increased carriage. After imputing missing data, the point estimate for carriage at 3 months was 8.6{\%} compared to 6.2{\%} at baseline (OR 1.42; 0.91 to 2.20). Recovery of N. meningitidis on selective agar was significantly reduced in cryovials frozen at 48 hours compared to 6 hours (24/26, 92.3{\%} vs. 14/26, 53.9{\%}, p = 0.002). Conclusion: Attending bars and engaging in intimate kissing is associated with oropharyngeal carriage in South Australian university students. Adolescent meningococcal vaccine programs should be implemented at school, prior to increased attendance at bars, intimate contact, and carriage acquisition. Delaying freezing of oropharyngeal specimens longer than 16 hours reduces yield of N. meningitidis by culture but not PCR detection.",
keywords = "adolescents, carriage, Neisseria meningitidis, risk factors, saliva",
author = "Mark McMillan and Luke Walters and Turra Mark and Andrew Lawrence and Leong, {Lex E.X.} and Thomas Sullivan and Rogers, {Geraint B.} and Andrews, {Ross M.} and Marshall, {Helen S.}",
year = "2019",
month = "4",
day = "3",
doi = "10.1080/21645515.2018.1551672",
language = "English",
volume = "15",
pages = "987--994",
journal = "Human Vaccines",
issn = "1554-8600",
publisher = "Taylor & Francis",
number = "4",

}

B Part of It study : a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia. / McMillan, Mark; Walters, Luke; Mark, Turra; Lawrence, Andrew; Leong, Lex E.X.; Sullivan, Thomas; Rogers, Geraint B.; Andrews, Ross M.; Marshall, Helen S.

In: Human Vaccines and Immunotherapeutics, Vol. 15, No. 4, 03.04.2019, p. 987-994.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - B Part of It study

T2 - a longitudinal study to assess carriage of Neisseria meningitidis in first year university students in South Australia

AU - McMillan, Mark

AU - Walters, Luke

AU - Mark, Turra

AU - Lawrence, Andrew

AU - Leong, Lex E.X.

AU - Sullivan, Thomas

AU - Rogers, Geraint B.

AU - Andrews, Ross M.

AU - Marshall, Helen S.

PY - 2019/4/3

Y1 - 2019/4/3

N2 - Objectives: N. meningitidis carriage in Australia is poorly understood. This study aimed to estimate prevalence and risk factors for carriage of N. meningitidis in South Australian university students. We also sought to identify whether delayed freezing of oropharyngeal samples altered PCR positivity, cycle threshold, or culture positivity. Methods: Oropharyngeal swabs were taken from first year university students and repeated after 3 months, with risk factor questionnaires completed at both visits. Specimens were subjected to real-time PCR screening for the presence of specific meningococcal DNA. Results: The study enrolled 421 individuals, 259 returned at 3 months. At baseline, 56% of participants were female and 1.9% smokers. Carriage of N. meningitidis at baseline was 6.2% (95% CI, [4.2%, 8.9%]). Visiting a bar more than once a week (OR 9.07; [2.44, 33.72]) and intimate kissing (OR 4.37; [1.45, 13.14]) were associated with increased carriage. After imputing missing data, the point estimate for carriage at 3 months was 8.6% compared to 6.2% at baseline (OR 1.42; 0.91 to 2.20). Recovery of N. meningitidis on selective agar was significantly reduced in cryovials frozen at 48 hours compared to 6 hours (24/26, 92.3% vs. 14/26, 53.9%, p = 0.002). Conclusion: Attending bars and engaging in intimate kissing is associated with oropharyngeal carriage in South Australian university students. Adolescent meningococcal vaccine programs should be implemented at school, prior to increased attendance at bars, intimate contact, and carriage acquisition. Delaying freezing of oropharyngeal specimens longer than 16 hours reduces yield of N. meningitidis by culture but not PCR detection.

AB - Objectives: N. meningitidis carriage in Australia is poorly understood. This study aimed to estimate prevalence and risk factors for carriage of N. meningitidis in South Australian university students. We also sought to identify whether delayed freezing of oropharyngeal samples altered PCR positivity, cycle threshold, or culture positivity. Methods: Oropharyngeal swabs were taken from first year university students and repeated after 3 months, with risk factor questionnaires completed at both visits. Specimens were subjected to real-time PCR screening for the presence of specific meningococcal DNA. Results: The study enrolled 421 individuals, 259 returned at 3 months. At baseline, 56% of participants were female and 1.9% smokers. Carriage of N. meningitidis at baseline was 6.2% (95% CI, [4.2%, 8.9%]). Visiting a bar more than once a week (OR 9.07; [2.44, 33.72]) and intimate kissing (OR 4.37; [1.45, 13.14]) were associated with increased carriage. After imputing missing data, the point estimate for carriage at 3 months was 8.6% compared to 6.2% at baseline (OR 1.42; 0.91 to 2.20). Recovery of N. meningitidis on selective agar was significantly reduced in cryovials frozen at 48 hours compared to 6 hours (24/26, 92.3% vs. 14/26, 53.9%, p = 0.002). Conclusion: Attending bars and engaging in intimate kissing is associated with oropharyngeal carriage in South Australian university students. Adolescent meningococcal vaccine programs should be implemented at school, prior to increased attendance at bars, intimate contact, and carriage acquisition. Delaying freezing of oropharyngeal specimens longer than 16 hours reduces yield of N. meningitidis by culture but not PCR detection.

KW - adolescents

KW - carriage

KW - Neisseria meningitidis

KW - risk factors

KW - saliva

UR - http://www.scopus.com/inward/record.url?scp=85059518573&partnerID=8YFLogxK

U2 - 10.1080/21645515.2018.1551672

DO - 10.1080/21645515.2018.1551672

M3 - Article

VL - 15

SP - 987

EP - 994

JO - Human Vaccines

JF - Human Vaccines

SN - 1554-8600

IS - 4

ER -