Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens

Heidi Luter, Stefanie Widder, E Botte, Muhammad Abdul Wahab, Steve Whalan, Lucas Moitinho-Silva, Torsten Thomas, Nicole Webster

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    Abstract

    Sponges are well known for hosting dense and diverse microbial communities, but how these associations vary with biogeography and environment is less clear. Here we compared the microbiome of an ecologically important sponge species, Carteriospongia foliascens, over a large geographic area and identified environmental factors likely responsible for driving microbial community differences between inshore and offshore locations using co-occurrence networks (NWs). The microbiome of C. foliascens exhibited exceptionally high microbial richness, with more than 9,000 OTUs identified at 97% sequence similarity. A large biogeographic signal was evident at the OTU level despite similar phyla level diversity being observed across all geographic locations. The C. foliascens bacterial community was primarily comprised of Gammaproteobacteria (34:2% � 3:4%) and Cyanobacteria (32:2% � 3:5%), with lower abundances of Alphaproteobacteria, Bacteroidetes, unidentified Proteobacteria, Actinobacteria, Acidobacteria and Deltaproteobacteria. Co-occurrence NWs revealed a consistent increase in the proportion of Cyanobacteria over Bacteroidetes between turbid inshore and oligotrophic offshore locations, suggesting that the specialist microbiome of C. foliascens is driven by environmental factors. � 2015 Luter et al.
    Original languageEnglish
    Article numbere1435
    Pages (from-to)1-17
    Number of pages17
    JournalPEERJ
    Volume3
    DOIs
    Publication statusPublished - 17 Dec 2015

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    Microbiota
    Porifera
    Bacteroidetes
    Cyanobacteria
    microbial communities
    Acidobacteria
    Deltaproteobacteria
    Otus
    delta-Proteobacteria
    Alphaproteobacteria
    Gammaproteobacteria
    Proteobacteria
    Geographic Locations
    environmental factors
    Actinobacteria
    alpha-Proteobacteria
    gamma-Proteobacteria
    bacterial communities
    biogeography
    microbiome

    Cite this

    Luter, H., Widder, S., Botte, E., Abdul Wahab, M., Whalan, S., Moitinho-Silva, L., ... Webster, N. (2015). Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens. PEERJ, 3, 1-17. [e1435]. https://doi.org/10.7717/peerj.1435
    Luter, Heidi ; Widder, Stefanie ; Botte, E ; Abdul Wahab, Muhammad ; Whalan, Steve ; Moitinho-Silva, Lucas ; Thomas, Torsten ; Webster, Nicole. / Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens. In: PEERJ. 2015 ; Vol. 3. pp. 1-17.
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    abstract = "Sponges are well known for hosting dense and diverse microbial communities, but how these associations vary with biogeography and environment is less clear. Here we compared the microbiome of an ecologically important sponge species, Carteriospongia foliascens, over a large geographic area and identified environmental factors likely responsible for driving microbial community differences between inshore and offshore locations using co-occurrence networks (NWs). The microbiome of C. foliascens exhibited exceptionally high microbial richness, with more than 9,000 OTUs identified at 97{\%} sequence similarity. A large biogeographic signal was evident at the OTU level despite similar phyla level diversity being observed across all geographic locations. The C. foliascens bacterial community was primarily comprised of Gammaproteobacteria (34:2{\%} � 3:4{\%}) and Cyanobacteria (32:2{\%} � 3:5{\%}), with lower abundances of Alphaproteobacteria, Bacteroidetes, unidentified Proteobacteria, Actinobacteria, Acidobacteria and Deltaproteobacteria. Co-occurrence NWs revealed a consistent increase in the proportion of Cyanobacteria over Bacteroidetes between turbid inshore and oligotrophic offshore locations, suggesting that the specialist microbiome of C. foliascens is driven by environmental factors. � 2015 Luter et al.",
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    Luter, H, Widder, S, Botte, E, Abdul Wahab, M, Whalan, S, Moitinho-Silva, L, Thomas, T & Webster, N 2015, 'Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens', PEERJ, vol. 3, e1435, pp. 1-17. https://doi.org/10.7717/peerj.1435

    Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens. / Luter, Heidi; Widder, Stefanie; Botte, E; Abdul Wahab, Muhammad; Whalan, Steve; Moitinho-Silva, Lucas; Thomas, Torsten; Webster, Nicole.

    In: PEERJ, Vol. 3, e1435, 17.12.2015, p. 1-17.

    Research output: Contribution to journalArticleResearchpeer-review

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    Luter H, Widder S, Botte E, Abdul Wahab M, Whalan S, Moitinho-Silva L et al. Biogeographic variation in the microbiome of the ecologically important sponge, Carteriospongia foliascens. PEERJ. 2015 Dec 17;3:1-17. e1435. https://doi.org/10.7717/peerj.1435