@article{91c98b2be8804b1ba37ee43e8928c3cd,
title = "BurkDiff: A real-time PCR allelic discrimination assay for Burkholderia Pseudomallei and B. mallei",
abstract = "A real-time PCR assay, BurkDiff, was designed to target a unique conserved region in the B. pseudomallei and B. mallei genomes containing a SNP that differentiates the two species. Sensitivity and specificity were assessed by screening BurkDiff across 469 isolates of B. pseudomallei, 49 isolates of B. mallei, and 390 isolates of clinically relevant non-target species. Concordance of results with traditional speciation methods and no cross-reactivity to non-target species show BurkDiff is a robust, highly validated assay for the detection and differentiation of B. pseudomallei and B. mallei. � 2010 Bowers et al.",
keywords = "allele, article, bacterium detection, bacterium isolate, BurkDiff, Burkholderia mallei, Burkholderia pseudomallei, controlled study, genetic screening, molecular probe, nonhuman, real time polymerase chain reaction, sensitivity and specificity, single nucleotide polymorphism, animal, bacterial genome, bacterium identification, classification, differential diagnosis, genetics, glanders, human, isolation and purification, melioidosis, methodology, microbiology, polymerase chain reaction, reproducibility, bacterial DNA, bacterial protein, Alleles, Animals, Bacterial Proteins, Bacterial Typing Techniques, Diagnosis, Differential, DNA, Bacterial, Genome, Bacterial, Glanders, Humans, Melioidosis, Polymerase Chain Reaction, Polymorphism, Single Nucleotide, Reproducibility of Results, Sensitivity and Specificity",
author = "Jolene Bowers and Engelthaler, {David M} and Ginther, {Jennifer L} and Talima Pearson and Peacock, {Sharon J} and Apichai Tuanyok and Wagner, {David M} and Bart Currie and Keim, {Paul S}",
year = "2010",
month = nov
day = "12",
doi = "10.1371/journal.pone.0015413",
language = "English",
volume = "5",
pages = "1--7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science (PLoS)",
number = "1",
}