Burkholderia humptydooensis sp. nov., a new species related to Burkholderia thailandensis and the fifth member of the Burkholderia pseudomallei complex

Apichai Tuanyok, Mark Mayo, Holger Scholz, Carina M. Hall, Christopher J. Allender, Mirjam Kaestli, Jennifer L Ginther, Senanu Spring-Pearson, Molly C. Bollig, Joshua K. Stone, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Jason W. Sahl, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Jay E. Gee, Richard A. Bowen, Jason T. LadnerSean Lovett, Galina Koroleva, Gustavo Palacios, David M Wagner, Bart J. Currie, Paul Keim

    Research output: Contribution to journalArticle

    Abstract

    During routine screening for Burkholderia pseudomallei from water wells in northern Australia in areas where it is endemic, Gram-negative bacteria (strains MSMB43T, MSMB121, and MSMB122) with a similar morphology and biochemical pattern to B. pseudomallei and B. thailandensis were coisolated with B. pseudomallei on Ashdown's selective agar. To determine the exact taxonomic position of these strains and to distinguish them from B. pseudomallei and B. thailandensis, they were subjected to a series of phenotypic and molecular analyses. Biochemical and fatty acid methyl ester analysis was unable to distinguish B. humptydooensis sp. nov. from closely related species. With matrix-assisted laser desorption ionization-time of flight analysis, all isolates grouped together in a cluster separate from other Burkholderia spp. 16S rRNA and recA sequence analyses demonstrated phylogenetic placement for B. humptydooensis sp. nov. in a novel clade within the B. pseudomallei group. Multilocus sequence typing (MLST) analysis of the three isolates in comparison with MLST data from 3,340 B. pseudomallei strains and related taxa revealed a new sequence type (ST318). Genome-to-genome distance calculations and the average nucleotide identity of all isolates to both B. thailandensis and B. pseudomallei, based on whole-genome sequences, also confirmed B. humptydooensis sp. nov. as a novel Burkholderia species within the B. pseudomallei complex. Molecular analyses clearly demonstrated that strains MSMB43T, MSMB121, and MSMB122 belong to a novel Burkholderia species for which the name Burkholderia humptydooensis sp. nov. is proposed, with the type strain MSMB43T (American Type Culture Collection BAA-2767; Belgian Co-ordinated Collections of Microorganisms LMG 29471; DDBJ accession numbers CP013380 to CP013382).

    Original languageEnglish
    Article numbere02802-16
    Pages (from-to)1-10
    Number of pages10
    JournalApplied and Environmental Microbiology
    Volume83
    Issue number5
    DOIs
    Publication statusPublished - 2017

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