Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

Brigit De Smet, Mark Mayo, Charlotte Peeters, James E. A. Zlosnik, Theodore Spilker, Trevor J. Hird, John LiPuma, Timothy Kidd, Mirjam Kaestli, Jennifer L Ginther, David M Wagner, Paul S Keim, Scott Bell, Jan A. Jacobs, Bart Currie, Peter Vandamme

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.
    Original languageEnglish
    Pages (from-to)2265-2271
    Number of pages7
    JournalInternational Journal of Systematic and Evolutionary Microbiology
    Volume65
    DOIs
    Publication statusPublished - 2015

    Fingerprint

    Burkholderia cepacia complex
    Burkholderia
    species complex
    new species
    bacterium
    gene
    ester
    nucleotide sequences
    analysis
    Sequence Analysis
    fatty acid
    divergence
    Burkholderia pseudomallei
    Northern Territory
    DNA
    Melioidosis
    Bacteria
    N-acetylglucosamine
    Maltose
    Acetylglucosamine

    Cite this

    De Smet, Brigit ; Mayo, Mark ; Peeters, Charlotte ; Zlosnik, James E. A. ; Spilker, Theodore ; Hird, Trevor J. ; LiPuma, John ; Kidd, Timothy ; Kaestli, Mirjam ; Ginther, Jennifer L ; Wagner, David M ; Keim, Paul S ; Bell, Scott ; Jacobs, Jan A. ; Currie, Bart ; Vandamme, Peter. / Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. In: International Journal of Systematic and Evolutionary Microbiology. 2015 ; Vol. 65. pp. 2265-2271.
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    title = "Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources",
    abstract = "Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9{\%}, respectively) and towards established Bcc species (4.0 and 3.9{\%}, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.",
    keywords = "DNA base, fatty acid ester, maltose, n acetylglucosamine, RNA 16S, bacterial DNA, fatty acid, Article, bacterium isolate, Burkholderia cepacia complex, Burkholderia stagnalis, Burkholderia territorii, DNA base composition, gene sequence, Gram negative bacterium, nonhuman, nucleotide sequence, phylogenetic tree, priority journal, random amplified polymorphic DNA, RecA gene, sequence analysis, type strain, Australia, bacterial gene, bacterium identification, chemistry, classification, DNA sequence, genetics, human, isolation and purification, microbiology, molecular genetics, multilocus sequence typing, phylogeny, sputum, Burkholderia, Burkholderia pseudomallei, Bacterial Typing Techniques, Base Composition, DNA, Bacterial, Fatty Acids, Genes, Bacterial, Humans, Molecular Sequence Data, Multilocus Sequence Typing, Northern Territory, Phylogeny, Random Amplified Polymorphic DNA Technique, RNA, Ribosomal, 16S, Sequence Analysis, DNA, Soil Microbiology, Sputum, Water Microbiology",
    author = "{De Smet}, Brigit and Mark Mayo and Charlotte Peeters and Zlosnik, {James E. A.} and Theodore Spilker and Hird, {Trevor J.} and John LiPuma and Timothy Kidd and Mirjam Kaestli and Ginther, {Jennifer L} and Wagner, {David M} and Keim, {Paul S} and Scott Bell and Jacobs, {Jan A.} and Bart Currie and Peter Vandamme",
    year = "2015",
    doi = "10.1099/ijs.0.000251",
    language = "English",
    volume = "65",
    pages = "2265--2271",
    journal = "International Journal of Systematic and Evolutionary Microbiology",
    issn = "1466-5026",
    publisher = "Global Science Books",

    }

    Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources. / De Smet, Brigit; Mayo, Mark; Peeters, Charlotte; Zlosnik, James E. A.; Spilker, Theodore; Hird, Trevor J.; LiPuma, John; Kidd, Timothy; Kaestli, Mirjam; Ginther, Jennifer L; Wagner, David M; Keim, Paul S; Bell, Scott; Jacobs, Jan A.; Currie, Bart; Vandamme, Peter.

    In: International Journal of Systematic and Evolutionary Microbiology, Vol. 65, 2015, p. 2265-2271.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from environmental and human sources

    AU - De Smet, Brigit

    AU - Mayo, Mark

    AU - Peeters, Charlotte

    AU - Zlosnik, James E. A.

    AU - Spilker, Theodore

    AU - Hird, Trevor J.

    AU - LiPuma, John

    AU - Kidd, Timothy

    AU - Kaestli, Mirjam

    AU - Ginther, Jennifer L

    AU - Wagner, David M

    AU - Keim, Paul S

    AU - Bell, Scott

    AU - Jacobs, Jan A.

    AU - Currie, Bart

    AU - Vandamme, Peter

    PY - 2015

    Y1 - 2015

    N2 - Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.

    AB - Nine Burkholderia cepacia complex (Bcc) bacteria were isolated during environmental surveys for the ecological niche of Burkholderia pseudomallei, the aetiological agent of melioidosis, in the Northern Territory of Australia. They represented two multi-locus sequence analysis-based clusters, referred to as Bcc B and Bcc L. Three additional environmental and clinical Bcc B isolates were identified upon deposition of the sequences in the PubMLST database. Analysis of the concatenated nucleotide sequence divergence levels within both groups (1.4 and 1.9%, respectively) and towards established Bcc species (4.0 and 3.9%, respectively) demonstrated that the two taxa represented novel Bcc species. All 12 isolates were further characterized using 16S rRNA and recA gene sequence analysis, RAPD analysis, DNA base content determination, fatty acid methyl ester analysis and biochemical profiling. Analysis of recA gene sequences revealed a remarkable diversity within each of these taxa, but, together, the results supported the affiliation of the two taxa to the Bcc. Bcc B strains can be differentiated from most other Bcc members by the assimilation of maltose. Bcc L strains can be differentiated from other Bcc members by the absence of assimilation of N-acetylglucosamine. The names Burkholderia stagnalis sp. nov. with type strain LMG 28156T (=CCUG 65686T) and Burkholderia territorii sp. nov. with type strain LMG 28158T (=CCUG 65687T) are proposed for Bcc B and Bcc L bacteria, respectively.

    KW - DNA base

    KW - fatty acid ester

    KW - maltose

    KW - n acetylglucosamine

    KW - RNA 16S

    KW - bacterial DNA

    KW - fatty acid

    KW - Article

    KW - bacterium isolate

    KW - Burkholderia cepacia complex

    KW - Burkholderia stagnalis

    KW - Burkholderia territorii

    KW - DNA base composition

    KW - gene sequence

    KW - Gram negative bacterium

    KW - nonhuman

    KW - nucleotide sequence

    KW - phylogenetic tree

    KW - priority journal

    KW - random amplified polymorphic DNA

    KW - RecA gene

    KW - sequence analysis

    KW - type strain

    KW - Australia

    KW - bacterial gene

    KW - bacterium identification

    KW - chemistry

    KW - classification

    KW - DNA sequence

    KW - genetics

    KW - human

    KW - isolation and purification

    KW - microbiology

    KW - molecular genetics

    KW - multilocus sequence typing

    KW - phylogeny

    KW - sputum

    KW - Burkholderia

    KW - Burkholderia pseudomallei

    KW - Bacterial Typing Techniques

    KW - Base Composition

    KW - DNA, Bacterial

    KW - Fatty Acids

    KW - Genes, Bacterial

    KW - Humans

    KW - Molecular Sequence Data

    KW - Multilocus Sequence Typing

    KW - Northern Territory

    KW - Phylogeny

    KW - Random Amplified Polymorphic DNA Technique

    KW - RNA, Ribosomal, 16S

    KW - Sequence Analysis, DNA

    KW - Soil Microbiology

    KW - Sputum

    KW - Water Microbiology

    U2 - 10.1099/ijs.0.000251

    DO - 10.1099/ijs.0.000251

    M3 - Article

    VL - 65

    SP - 2265

    EP - 2271

    JO - International Journal of Systematic and Evolutionary Microbiology

    JF - International Journal of Systematic and Evolutionary Microbiology

    SN - 1466-5026

    ER -