Clinical and biochemical tear lipid parameters in contact lens wearers

Athira Rohit, Mark D.P. Willcox, Simon H.J. Brown, Todd W. Mitchell, Fiona Stapleton

    Research output: Contribution to journalArticle

    Abstract

    Purpose: Alterations in tear film lipids may be important in modulating discomfort during contact lens wear. This study investigates associations between clinical and biological components of the lipid layer and seeks to determine the effect of lipid supplementation on contact lens wear comfort. 

    Methods: Participants were grouped into symptomatic (n = 10) and asymptomatic (n = 10) contact lens wearers according to the Contact Lens Dry Eye Questionnaire. After 6 hours of lens wear, noninvasive surface drying time (NISDT) and the lipid layer grade were assessed using a Tearscope. Basal tears were collected using a microcapillary tube and assayed for concentration and activity of the secretory phospholipase A2 enzyme and concentration of the lipid aldehyde malondialdehyde. Mass spectrometry was used to characterize the tear lipidome. In the second phase, a liposomal spray (Tears Again, BioRevive) or a saline spray was sprayed over the upper eyelids of each subject during their down gaze and during lens wear. Noninvasive surface drying time and ocular comfort were obtained soon after spraying and again at 2 and 6 hours after the initial spray. Statistical tests included the Student t test, repeated-measures analysis of variance, and the Pearson correlation test where appropriate. 

    Results: Noninvasive surface drying time was lower (p = 0.01) in symptomatic (4.5 ± 0.6 seconds) than in asymptomatic (9.9 ± 3.1 seconds) contact lens wearers. The mole percentage of wax esters in the total lipidome increased with NISDT (R2 = 0.70, p = 0.01). Secretory phospholipase A2 enzyme activity in tearswas associatedwith higher levels ofmalondialdehyde (R2 = 0.65, p = 0.01) and shorterNISDT (R2 = 0.84, p = 0.001).Noninvasive surface drying time reduced over the time course for the saline spray (p = 0.01) but did not reduce until the 6-hour time point with the liposomal spray. With liposomal spray, NISDT was higher (p = 0.03) immediately after instillation compared with 6 hours later (9.5 ± 1.9 vs. 5.2 ± 2.1 seconds). A longer NISDT was associated with improved ocular comfort for those using the liposomal spray (R2 = 0.25, p = 0.005) but not with saline. 

    Conclusions: Degraded lipids and a lower mole percentage of wax esters in the tear film may be associated with a lower NISDT. Lipid supplements may improve ocular comfort during lens wear by increasing NISDT.

    Original languageEnglish
    Pages (from-to)1384-1390
    Number of pages7
    JournalOptometry and Vision Science
    Volume91
    Issue number12
    DOIs
    Publication statusPublished - 1 Jan 2014

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    Rohit, A., Willcox, M. D. P., Brown, S. H. J., Mitchell, T. W., & Stapleton, F. (2014). Clinical and biochemical tear lipid parameters in contact lens wearers. Optometry and Vision Science, 91(12), 1384-1390. https://doi.org/10.1097/OPX.0000000000000420