Customary and commercial use of wildlife supports millions of Indigenous people world-wide. However, in Australia, despite there being abundant natural resources and intricate Aboriginal ecological knowledge, there are relatively few financially viable Aboriginal wildlife-based enterprises. This research provides an example of how the Collective Impact (CI) Framework was used to retrospectively analyse the development of an Aboriginal enterprise in the Northern Territory of Australia over a fifteen-year period. We identified many parallels between the elements of the CI approach and the various development phases of this Aboriginal enterprise. This research demonstrates that there is a complex interplay of factors that influence the development of Indigenous enterprise in remote communities of northern Australia, and that the CI Framework can be useful for reflecting on this work because of its focus on collaboration with diverse stakeholders. It contributes to knowledge about the benefits and the difficulties of meaningful whole of community engagement as a core aspect of enterprise development.