Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens

Stephen B Lambert, K Allen, J Druce, C Birch, Ian M Mackay, J CARLIN, Jonathan Carapetis, Theo P Sloots, Michael D Nissen, Terry Nolan

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    OBJECTIVES. The purpose of this work was to assess the impact of recently described human metapneumovirus and human coronavirus NL63 compared with other respiratory viruses by using sensitive molecular techniques in a cohort of healthy preschool-aged children. We also aimed to assess the use of parent collection to obtain an adequate respiratory specimen from acutely unwell children in the community. PATIENTS AND METHODS. The community epidemiology and burden of human metapneumovirus and other respiratory viruses (influenza A, influenza B, respiratory syncytial virus, parainfluenza viruses, adenoviruses, and picornaviruses) were examined in a cohort of 234 preschool-aged children from Melbourne, Australia, over a 12-month period by using polymerase chain reaction testing. Parents collected a daily symptom diary for the duration of the study and were taught to collect a combined nose-throat swab and complete an impact diary when the study child had an acute respiratory illness. RESULTS. The average incidence of acute respiratory illness was 0.48 per child-month for the duration of the study, with a winter peak. Of 543 illnesses with ?1 specimen returned, 33 were positive for human metapneumovirus (6.1%) and 18 for human coronavirus NL63 (3.3%). Of all of the viruses for which we tested, human metapneumovirus and human coronavirus NL63 were most strongly linked to child care attendance, occurring in 82% and 78% of infected children, respectively. Picornaviruses were the most commonly identified virus group (269 [49.5%]). Influenza virus and adenovirus illnesses had the greatest impact, with fever in more than three quarters and requiring, on average, >1 local doctor visit per illness. CONCLUSIONS. Recently identified human metapneumovirus and human coronavirus NL63 are important pathogens in community-based illness in children, particularly in those who attend child care. Picornaviruses were detected in half of the nose-throat swabs collected during acute respiratory illness in children but resulted in milder illnesses; influenza and adenovirus caused the highest-impact illnesses. The use of parent-collected specimens should be considered for additional community-based epidemiologic studies and vaccine trials. Copyright � 2007 by the American Academy of Pediatrics.
    Original languageEnglish
    Pages (from-to)-
    JournalPediatrics
    Volume120
    Issue number4
    Publication statusPublished - 2007

    Fingerprint

    Human Coronavirus NL63
    Metapneumovirus
    Preschool Children
    Epidemiology
    Viruses
    Picornaviridae
    Adenoviridae
    Child Care
    Pharynx
    Nose
    Human Influenza
    Paramyxoviridae Infections
    Respiratory Syncytial Viruses
    Influenza A virus
    Orthomyxoviridae
    Epidemiologic Studies
    Fever
    Vaccines
    Parents
    Polymerase Chain Reaction

    Cite this

    Lambert, Stephen B ; Allen, K ; Druce, J ; Birch, C ; Mackay, Ian M ; CARLIN, J ; Carapetis, Jonathan ; Sloots, Theo P ; Nissen, Michael D ; Nolan, Terry. / Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens. In: Pediatrics. 2007 ; Vol. 120, No. 4. pp. -.
    @article{7a87732f8fc14bdc8939f4812ce23e1c,
    title = "Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens",
    abstract = "OBJECTIVES. The purpose of this work was to assess the impact of recently described human metapneumovirus and human coronavirus NL63 compared with other respiratory viruses by using sensitive molecular techniques in a cohort of healthy preschool-aged children. We also aimed to assess the use of parent collection to obtain an adequate respiratory specimen from acutely unwell children in the community. PATIENTS AND METHODS. The community epidemiology and burden of human metapneumovirus and other respiratory viruses (influenza A, influenza B, respiratory syncytial virus, parainfluenza viruses, adenoviruses, and picornaviruses) were examined in a cohort of 234 preschool-aged children from Melbourne, Australia, over a 12-month period by using polymerase chain reaction testing. Parents collected a daily symptom diary for the duration of the study and were taught to collect a combined nose-throat swab and complete an impact diary when the study child had an acute respiratory illness. RESULTS. The average incidence of acute respiratory illness was 0.48 per child-month for the duration of the study, with a winter peak. Of 543 illnesses with ?1 specimen returned, 33 were positive for human metapneumovirus (6.1{\%}) and 18 for human coronavirus NL63 (3.3{\%}). Of all of the viruses for which we tested, human metapneumovirus and human coronavirus NL63 were most strongly linked to child care attendance, occurring in 82{\%} and 78{\%} of infected children, respectively. Picornaviruses were the most commonly identified virus group (269 [49.5{\%}]). Influenza virus and adenovirus illnesses had the greatest impact, with fever in more than three quarters and requiring, on average, >1 local doctor visit per illness. CONCLUSIONS. Recently identified human metapneumovirus and human coronavirus NL63 are important pathogens in community-based illness in children, particularly in those who attend child care. Picornaviruses were detected in half of the nose-throat swabs collected during acute respiratory illness in children but resulted in milder illnesses; influenza and adenovirus caused the highest-impact illnesses. The use of parent-collected specimens should be considered for additional community-based epidemiologic studies and vaccine trials. Copyright � 2007 by the American Academy of Pediatrics.",
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    author = "Lambert, {Stephen B} and K Allen and J Druce and C Birch and Mackay, {Ian M} and J CARLIN and Jonathan Carapetis and Sloots, {Theo P} and Nissen, {Michael D} and Terry Nolan",
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    Lambert, SB, Allen, K, Druce, J, Birch, C, Mackay, IM, CARLIN, J, Carapetis, J, Sloots, TP, Nissen, MD & Nolan, T 2007, 'Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens', Pediatrics, vol. 120, no. 4, pp. -.

    Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens. / Lambert, Stephen B; Allen, K; Druce, J; Birch, C; Mackay, Ian M; CARLIN, J; Carapetis, Jonathan; Sloots, Theo P; Nissen, Michael D; Nolan, Terry.

    In: Pediatrics, Vol. 120, No. 4, 2007, p. -.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Community Epidemiology of Human Metapneumovirus, Human Coronavirus NL63, and Other Respiratory Viruses in Healthy Preschool-Aged Children Using Parent-Collected Specimens

    AU - Lambert, Stephen B

    AU - Allen, K

    AU - Druce, J

    AU - Birch, C

    AU - Mackay, Ian M

    AU - CARLIN, J

    AU - Carapetis, Jonathan

    AU - Sloots, Theo P

    AU - Nissen, Michael D

    AU - Nolan, Terry

    PY - 2007

    Y1 - 2007

    N2 - OBJECTIVES. The purpose of this work was to assess the impact of recently described human metapneumovirus and human coronavirus NL63 compared with other respiratory viruses by using sensitive molecular techniques in a cohort of healthy preschool-aged children. We also aimed to assess the use of parent collection to obtain an adequate respiratory specimen from acutely unwell children in the community. PATIENTS AND METHODS. The community epidemiology and burden of human metapneumovirus and other respiratory viruses (influenza A, influenza B, respiratory syncytial virus, parainfluenza viruses, adenoviruses, and picornaviruses) were examined in a cohort of 234 preschool-aged children from Melbourne, Australia, over a 12-month period by using polymerase chain reaction testing. Parents collected a daily symptom diary for the duration of the study and were taught to collect a combined nose-throat swab and complete an impact diary when the study child had an acute respiratory illness. RESULTS. The average incidence of acute respiratory illness was 0.48 per child-month for the duration of the study, with a winter peak. Of 543 illnesses with ?1 specimen returned, 33 were positive for human metapneumovirus (6.1%) and 18 for human coronavirus NL63 (3.3%). Of all of the viruses for which we tested, human metapneumovirus and human coronavirus NL63 were most strongly linked to child care attendance, occurring in 82% and 78% of infected children, respectively. Picornaviruses were the most commonly identified virus group (269 [49.5%]). Influenza virus and adenovirus illnesses had the greatest impact, with fever in more than three quarters and requiring, on average, >1 local doctor visit per illness. CONCLUSIONS. Recently identified human metapneumovirus and human coronavirus NL63 are important pathogens in community-based illness in children, particularly in those who attend child care. Picornaviruses were detected in half of the nose-throat swabs collected during acute respiratory illness in children but resulted in milder illnesses; influenza and adenovirus caused the highest-impact illnesses. The use of parent-collected specimens should be considered for additional community-based epidemiologic studies and vaccine trials. Copyright � 2007 by the American Academy of Pediatrics.

    AB - OBJECTIVES. The purpose of this work was to assess the impact of recently described human metapneumovirus and human coronavirus NL63 compared with other respiratory viruses by using sensitive molecular techniques in a cohort of healthy preschool-aged children. We also aimed to assess the use of parent collection to obtain an adequate respiratory specimen from acutely unwell children in the community. PATIENTS AND METHODS. The community epidemiology and burden of human metapneumovirus and other respiratory viruses (influenza A, influenza B, respiratory syncytial virus, parainfluenza viruses, adenoviruses, and picornaviruses) were examined in a cohort of 234 preschool-aged children from Melbourne, Australia, over a 12-month period by using polymerase chain reaction testing. Parents collected a daily symptom diary for the duration of the study and were taught to collect a combined nose-throat swab and complete an impact diary when the study child had an acute respiratory illness. RESULTS. The average incidence of acute respiratory illness was 0.48 per child-month for the duration of the study, with a winter peak. Of 543 illnesses with ?1 specimen returned, 33 were positive for human metapneumovirus (6.1%) and 18 for human coronavirus NL63 (3.3%). Of all of the viruses for which we tested, human metapneumovirus and human coronavirus NL63 were most strongly linked to child care attendance, occurring in 82% and 78% of infected children, respectively. Picornaviruses were the most commonly identified virus group (269 [49.5%]). Influenza virus and adenovirus illnesses had the greatest impact, with fever in more than three quarters and requiring, on average, >1 local doctor visit per illness. CONCLUSIONS. Recently identified human metapneumovirus and human coronavirus NL63 are important pathogens in community-based illness in children, particularly in those who attend child care. Picornaviruses were detected in half of the nose-throat swabs collected during acute respiratory illness in children but resulted in milder illnesses; influenza and adenovirus caused the highest-impact illnesses. The use of parent-collected specimens should be considered for additional community-based epidemiologic studies and vaccine trials. Copyright � 2007 by the American Academy of Pediatrics.

    KW - acute respiratory tract disease

    KW - Adenovirus

    KW - article

    KW - Australia

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    KW - Coronavirus

    KW - female

    KW - fever

    KW - human

    KW - Human metapneumovirus

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    KW - Influenza virus A

    KW - Influenza virus B

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    KW - nose smear

    KW - Parainfluenza virus

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    KW - Respiratory syncytial pneumovirus

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    KW - Cohort Studies

    KW - Female

    KW - Fever

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    KW - Infant

    KW - Male

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    VL - 120

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    JO - Pediatrics

    JF - Pediatrics

    SN - 0031-4005

    IS - 4

    ER -