Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection Of The Genus Burkholderia

Peter Vandamme, Charlotte Peeters, Birgit De Smet, Erin P. Price, Derek S. Sarovich, Deborah A. Henry, Trevor J. Hird, James E.A. Zlosnik, Mark Mayo, Jeffrey Warner, Anthony Baker, Bart J. Currie, Aurélien Carlier

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    Abstract

    Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. The type strain is LMG 28154T (DCCUG 65685T). Its genome sequence has an average mol% GCC content of 64.34%, which is considerably lower than that of other Burkholderia species. The reduced GCC content of strain LMG 28154T was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents.

    Original languageEnglish
    Article number1679
    Pages (from-to)1-14
    Number of pages14
    JournalFrontiers in Microbiology
    Volume8
    DOIs
    Publication statusPublished - Sep 2017

    Fingerprint

    Burkholderia
    Base Composition
    Genomics
    Dissection
    Genome
    Bacteria
    Burkholderia pseudomallei
    Multilocus Sequence Typing
    Gene Conversion
    Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
    DNA Repair
    Nitrates
    Agar
    Names
    Mass Spectrometry
    Sheep
    Oxidoreductases
    Water
    DNA

    Cite this

    Vandamme, Peter ; Peeters, Charlotte ; Smet, Birgit De ; Price, Erin P. ; Sarovich, Derek S. ; Henry, Deborah A. ; Hird, Trevor J. ; Zlosnik, James E.A. ; Mayo, Mark ; Warner, Jeffrey ; Baker, Anthony ; Currie, Bart J. ; Carlier, Aurélien. / Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection Of The Genus Burkholderia. In: Frontiers in Microbiology. 2017 ; Vol. 8. pp. 1-14.
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    abstract = "Four Burkholderia pseudomallei-like isolates of human clinical origin were examined by a polyphasic taxonomic approach that included comparative whole genome analyses. The results demonstrated that these isolates represent a rare and unusual, novel Burkholderia species for which we propose the name B. singularis. The type strain is LMG 28154T (DCCUG 65685T). Its genome sequence has an average mol{\%} GCC content of 64.34{\%}, which is considerably lower than that of other Burkholderia species. The reduced GCC content of strain LMG 28154T was characterized by a genome wide AT bias that was not due to reduced GC-biased gene conversion or reductive genome evolution, but might have been caused by an altered DNA base excision repair pathway. B. singularis can be differentiated from other Burkholderia species by multilocus sequence analysis, MALDI-TOF mass spectrometry and a distinctive biochemical profile that includes the absence of nitrate reduction, a mucoid appearance on Columbia sheep blood agar, and a slowly positive oxidase reaction. Comparisons with publicly available whole genome sequences demonstrated that strain TSV85, an Australian water isolate, also represents the same species and therefore, to date, B. singularis has been recovered from human or environmental samples on three continents.",
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    author = "Peter Vandamme and Charlotte Peeters and Smet, {Birgit De} and Price, {Erin P.} and Sarovich, {Derek S.} and Henry, {Deborah A.} and Hird, {Trevor J.} and Zlosnik, {James E.A.} and Mark Mayo and Jeffrey Warner and Anthony Baker and Currie, {Bart J.} and Aur{\'e}lien Carlier",
    year = "2017",
    month = "9",
    doi = "10.3389/fmicb.2017.01679",
    language = "English",
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    Vandamme, P, Peeters, C, Smet, BD, Price, EP, Sarovich, DS, Henry, DA, Hird, TJ, Zlosnik, JEA, Mayo, M, Warner, J, Baker, A, Currie, BJ & Carlier, A 2017, 'Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection Of The Genus Burkholderia', Frontiers in Microbiology, vol. 8, 1679, pp. 1-14. https://doi.org/10.3389/fmicb.2017.01679

    Comparative Genomics of Burkholderia singularis sp. nov., a Low G+C Content, Free-Living Bacterium That Defies Taxonomic Dissection Of The Genus Burkholderia. / Vandamme, Peter; Peeters, Charlotte; Smet, Birgit De; Price, Erin P.; Sarovich, Derek S.; Henry, Deborah A.; Hird, Trevor J.; Zlosnik, James E.A.; Mayo, Mark; Warner, Jeffrey; Baker, Anthony; Currie, Bart J.; Carlier, Aurélien.

    In: Frontiers in Microbiology, Vol. 8, 1679, 09.2017, p. 1-14.

    Research output: Contribution to journalArticleResearchpeer-review

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