The use of Mueller-Hinton agar supplemented with citrated human or citrated sheep blood was compared with the use of routinely used Mueller-Hinton agar supplemented with defibrinated sheep blood for antimicrobial susceptibility testing of Streptococcus pneumoniae. The alternate supplements were found to be unsatisfactory, particularly for testing resistant isolates, and therefore are not recommended.
Satzke, C., Seduadua, A., Chandra, R., Carapetis, J., MULHOLLAND, E. K., & Russell, F. (2010). Comparison of citrated human blood, citrated sheep blood, and defibrinated sheep blood Mueller-Hinton agar preparations for antimicrobial susceptibility testing of streptococcus pneumoniae isolates. Journal of Clinical Microbiology, 48(10), 3770-3772. https://doi.org/10.1128/JCM.02357-09