Comparison of fin and muscle tissues for analysis of signature fatty acids in tropical euryhaline sharks

Sharon Louise Every, Heidi Pethybridge, David Crook, Peter Kyne, Christopher Fulton

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Fatty acid (FA) analysis can provide an effective, non-lethal method of elucidating the trophic ecology of fish. One method utilised in the field is to collect biopsied muscle tissue, but this can be problematic in live sharks due to a thick dermal layer with extensive connective tissue. The aim of this research was to determine whether fin and muscle tissue yield similar FA profiles in three species of tropical euryhaline sharks: Carcharhinus leucas, Glyphis garricki and Glyphis glyphis. Fatty acid profiles were detectable in fin clips as small as 20 mg (~. 5 mm x 6 mm) and muscle biopsies >. 10 mg mass. Overall profiles in relative (%) FA composition varied significantly between fin and muscle tissues for C. leucas and G. garricki (global R-values = 0.204 and 0.195, P < 0.01), but not G. glyphis (global R-value = 0.063, P = 0.257). The main FAs that contributed to these differences were largely 18:0 for C. leucas, 20:4 ?6 for G. garricki and 20:5 ?3 for G. glyphis, which reflect the different physiological functions and turnover rates of the two tissues. Notably, no significant differences were detected between tissue types for the major classes of FAs and abundant dietary essential FAs. It was concluded that FA profiles from either fin clips or muscle tissue may be used to examine the trophic ecology of these tropical euryhaline sharks when focusing on dietary essential FAs. Given that some non-essential FAs were different, caution should be applied when comparing FA profiles across different tissue types. 
    Original languageEnglish
    Pages (from-to)46-53
    Number of pages8
    JournalJournal of Experimental Marine Biology and Ecology
    Volume479
    DOIs
    Publication statusPublished - Jun 2016

    Fingerprint

    tissue analysis
    shark
    sharks
    muscle tissues
    fins
    Leucas
    muscle
    fatty acid
    fatty acid composition
    fatty acids
    ecology
    Carcharhinus
    connective tissues
    biopsy
    analysis
    comparison
    tissue
    muscles
    turnover
    methodology

    Cite this

    @article{9fe1b9978f744aeb9ee30ecf35640a01,
    title = "Comparison of fin and muscle tissues for analysis of signature fatty acids in tropical euryhaline sharks",
    abstract = "Fatty acid (FA) analysis can provide an effective, non-lethal method of elucidating the trophic ecology of fish. One method utilised in the field is to collect biopsied muscle tissue, but this can be problematic in live sharks due to a thick dermal layer with extensive connective tissue. The aim of this research was to determine whether fin and muscle tissue yield similar FA profiles in three species of tropical euryhaline sharks: Carcharhinus leucas, Glyphis garricki and Glyphis glyphis. Fatty acid profiles were detectable in fin clips as small as 20 mg (~. 5 mm x 6 mm) and muscle biopsies >. 10 mg mass. Overall profiles in relative ({\%}) FA composition varied significantly between fin and muscle tissues for C. leucas and G. garricki (global R-values = 0.204 and 0.195, P < 0.01), but not G. glyphis (global R-value = 0.063, P = 0.257). The main FAs that contributed to these differences were largely 18:0 for C. leucas, 20:4 ?6 for G. garricki and 20:5 ?3 for G. glyphis, which reflect the different physiological functions and turnover rates of the two tissues. Notably, no significant differences were detected between tissue types for the major classes of FAs and abundant dietary essential FAs. It was concluded that FA profiles from either fin clips or muscle tissue may be used to examine the trophic ecology of these tropical euryhaline sharks when focusing on dietary essential FAs. Given that some non-essential FAs were different, caution should be applied when comparing FA profiles across different tissue types. ",
    keywords = "Carcharhinus, Carcharhinus leucas, Chondrichthyes, Elasmobranchii, Glyphis, Glyphis glyphis",
    author = "Every, {Sharon Louise} and Heidi Pethybridge and David Crook and Peter Kyne and Christopher Fulton",
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    month = "6",
    doi = "10.1016/j.jembe.2016.02.011",
    language = "English",
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    pages = "46--53",
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    Comparison of fin and muscle tissues for analysis of signature fatty acids in tropical euryhaline sharks. / Every, Sharon Louise; Pethybridge, Heidi; Crook, David; Kyne, Peter; Fulton, Christopher.

    In: Journal of Experimental Marine Biology and Ecology, Vol. 479, 06.2016, p. 46-53.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Comparison of fin and muscle tissues for analysis of signature fatty acids in tropical euryhaline sharks

    AU - Every, Sharon Louise

    AU - Pethybridge, Heidi

    AU - Crook, David

    AU - Kyne, Peter

    AU - Fulton, Christopher

    PY - 2016/6

    Y1 - 2016/6

    N2 - Fatty acid (FA) analysis can provide an effective, non-lethal method of elucidating the trophic ecology of fish. One method utilised in the field is to collect biopsied muscle tissue, but this can be problematic in live sharks due to a thick dermal layer with extensive connective tissue. The aim of this research was to determine whether fin and muscle tissue yield similar FA profiles in three species of tropical euryhaline sharks: Carcharhinus leucas, Glyphis garricki and Glyphis glyphis. Fatty acid profiles were detectable in fin clips as small as 20 mg (~. 5 mm x 6 mm) and muscle biopsies >. 10 mg mass. Overall profiles in relative (%) FA composition varied significantly between fin and muscle tissues for C. leucas and G. garricki (global R-values = 0.204 and 0.195, P < 0.01), but not G. glyphis (global R-value = 0.063, P = 0.257). The main FAs that contributed to these differences were largely 18:0 for C. leucas, 20:4 ?6 for G. garricki and 20:5 ?3 for G. glyphis, which reflect the different physiological functions and turnover rates of the two tissues. Notably, no significant differences were detected between tissue types for the major classes of FAs and abundant dietary essential FAs. It was concluded that FA profiles from either fin clips or muscle tissue may be used to examine the trophic ecology of these tropical euryhaline sharks when focusing on dietary essential FAs. Given that some non-essential FAs were different, caution should be applied when comparing FA profiles across different tissue types. 

    AB - Fatty acid (FA) analysis can provide an effective, non-lethal method of elucidating the trophic ecology of fish. One method utilised in the field is to collect biopsied muscle tissue, but this can be problematic in live sharks due to a thick dermal layer with extensive connective tissue. The aim of this research was to determine whether fin and muscle tissue yield similar FA profiles in three species of tropical euryhaline sharks: Carcharhinus leucas, Glyphis garricki and Glyphis glyphis. Fatty acid profiles were detectable in fin clips as small as 20 mg (~. 5 mm x 6 mm) and muscle biopsies >. 10 mg mass. Overall profiles in relative (%) FA composition varied significantly between fin and muscle tissues for C. leucas and G. garricki (global R-values = 0.204 and 0.195, P < 0.01), but not G. glyphis (global R-value = 0.063, P = 0.257). The main FAs that contributed to these differences were largely 18:0 for C. leucas, 20:4 ?6 for G. garricki and 20:5 ?3 for G. glyphis, which reflect the different physiological functions and turnover rates of the two tissues. Notably, no significant differences were detected between tissue types for the major classes of FAs and abundant dietary essential FAs. It was concluded that FA profiles from either fin clips or muscle tissue may be used to examine the trophic ecology of these tropical euryhaline sharks when focusing on dietary essential FAs. Given that some non-essential FAs were different, caution should be applied when comparing FA profiles across different tissue types. 

    KW - Carcharhinus

    KW - Carcharhinus leucas

    KW - Chondrichthyes

    KW - Elasmobranchii

    KW - Glyphis

    KW - Glyphis glyphis

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    U2 - 10.1016/j.jembe.2016.02.011

    DO - 10.1016/j.jembe.2016.02.011

    M3 - Article

    VL - 479

    SP - 46

    EP - 53

    JO - Journal of Experimental Marine Biology and Ecology

    JF - Journal of Experimental Marine Biology and Ecology

    SN - 0022-0981

    ER -