@article{47697fa15b3d4a0a89e16f938c123ad1,
title = "Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens",
abstract = "Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin. ",
keywords = "article, bacterial gene, bacterium detection, blood sampling, Burkholderia pseudomallei, clinical evaluation, controlled study, gene targeting, geographic origin, human, intermethod comparison, laboratory diagnosis, limit of detection, melioidosis, molecular diagnosis, nonhuman, open reading frame, polymerase chain reaction, priority journal, real time polymerase chain reaction, sensitivity and specificity, sputum analysis, targeting the type III secretion 1 assay, urinalysis, Yersinia like fimbrial Burkholderia thailandensis like flagellum and chemotaxis region assay, Bacteriological Techniques, Humans, Melioidosis, Molecular Diagnostic Techniques, Polymerase Chain Reaction, Sensitivity and Specificity, Bacteria (microorganisms), Yersinia",
author = "Mirjam Kaestli and Richardson, {Leisha Jade} and Rebecca Colman and Apichai Tuanyok and Erin Price and Jolene Bowers and Mark Mayo and Erin Kelley and Meagan Seymour and Derek Sarovich and Talima Pearson and Engelthaler, {David M} and Wagner, {David M} and Keim, {Paul S} and James Schupp and Bart Currie",
year = "2012",
month = jun,
doi = "10.1128/JCM.06737-11",
language = "English",
volume = "50",
pages = "2059--2062",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "6",
}
