Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

Mirjam Kaestli, Leisha Jade Richardson, Rebecca Colman, Apichai Tuanyok, Erin Price, Jolene Bowers, Mark Mayo, Erin Kelley, Meagan Seymour, Derek Sarovich, Talima Pearson, David M Engelthaler, David M Wagner, Paul S Keim, James Schupp, Bart Currie

    Research output: Contribution to journalArticle

    Abstract

    Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.
    Original languageEnglish
    Pages (from-to)2059-2062
    Number of pages4
    JournalJournal of Clinical Microbiology
    Volume50
    Issue number6
    DOIs
    Publication statusPublished - Jun 2012

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    Kaestli, M., Richardson, L. J., Colman, R., Tuanyok, A., Price, E., Bowers, J., Mayo, M., Kelley, E., Seymour, M., Sarovich, D., Pearson, T., Engelthaler, D. M., Wagner, D. M., Keim, P. S., Schupp, J., & Currie, B. (2012). Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens. Journal of Clinical Microbiology, 50(6), 2059-2062. https://doi.org/10.1128/JCM.06737-11