Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens

Mirjam Kaestli, Leisha Jade Richardson, Rebecca Colman, Apichai Tuanyok, Erin Price, Jolene Bowers, Mark Mayo, Erin Kelley, Meagan Seymour, Derek Sarovich, Talima Pearson, David M Engelthaler, David M Wagner, Paul S Keim, James Schupp, Bart Currie

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.
    Original languageEnglish
    Pages (from-to)2059-2062
    Number of pages4
    JournalJournal of Clinical Microbiology
    Volume50
    Issue number6
    DOIs
    Publication statusPublished - Jun 2012

    Fingerprint

    Melioidosis
    Burkholderia pseudomallei
    Emerging Communicable Diseases
    Burkholderia
    Yersinia
    Polymerase Chain Reaction
    Flagella
    Chemotaxis
    Limit of Detection
    Soil
    Bacteria

    Cite this

    Kaestli, Mirjam ; Richardson, Leisha Jade ; Colman, Rebecca ; Tuanyok, Apichai ; Price, Erin ; Bowers, Jolene ; Mayo, Mark ; Kelley, Erin ; Seymour, Meagan ; Sarovich, Derek ; Pearson, Talima ; Engelthaler, David M ; Wagner, David M ; Keim, Paul S ; Schupp, James ; Currie, Bart. / Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens. In: Journal of Clinical Microbiology. 2012 ; Vol. 50, No. 6. pp. 2059-2062.
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    title = "Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens",
    abstract = "Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.",
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    author = "Mirjam Kaestli and Richardson, {Leisha Jade} and Rebecca Colman and Apichai Tuanyok and Erin Price and Jolene Bowers and Mark Mayo and Erin Kelley and Meagan Seymour and Derek Sarovich and Talima Pearson and Engelthaler, {David M} and Wagner, {David M} and Keim, {Paul S} and James Schupp and Bart Currie",
    year = "2012",
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    doi = "10.1128/JCM.06737-11",
    language = "English",
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    Kaestli, M, Richardson, LJ, Colman, R, Tuanyok, A, Price, E, Bowers, J, Mayo, M, Kelley, E, Seymour, M, Sarovich, D, Pearson, T, Engelthaler, DM, Wagner, DM, Keim, PS, Schupp, J & Currie, B 2012, 'Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens', Journal of Clinical Microbiology, vol. 50, no. 6, pp. 2059-2062. https://doi.org/10.1128/JCM.06737-11

    Comparison of TaqMan PCR Assays for Detection of the Melioidosis Agent Burkholderia pseudomallei in Clinical Specimens. / Kaestli, Mirjam; Richardson, Leisha Jade; Colman, Rebecca; Tuanyok, Apichai; Price, Erin; Bowers, Jolene; Mayo, Mark; Kelley, Erin; Seymour, Meagan; Sarovich, Derek; Pearson, Talima; Engelthaler, David M; Wagner, David M; Keim, Paul S; Schupp, James; Currie, Bart.

    In: Journal of Clinical Microbiology, Vol. 50, No. 6, 06.2012, p. 2059-2062.

    Research output: Contribution to journalArticleResearchpeer-review

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    AU - Price, Erin

    AU - Bowers, Jolene

    AU - Mayo, Mark

    AU - Kelley, Erin

    AU - Seymour, Meagan

    AU - Sarovich, Derek

    AU - Pearson, Talima

    AU - Engelthaler, David M

    AU - Wagner, David M

    AU - Keim, Paul S

    AU - Schupp, James

    AU - Currie, Bart

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    AB - Melioidosis is an emerging infectious disease caused by the soil bacterium Burkholderia pseudomallei. In diagnostic and forensic settings, molecular detection assays need not only high sensitivity with low limits of detection but also high specificity. In a direct comparison of published and newly developed TaqMan PCR assays, we found the TTS1-orf2 assay to be superior in detecting B. pseudomallei directly from clinical specimens. The YLF/BTFC multiplex assay (targeting the Yersinia-like fimbrial/Burkholderia thailandensis-like flagellum and chemotaxis region) also showed high diagnostic sensitivity and provides additional information on possible geographic origin.

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