TY - JOUR
T1 - Cost-effective interrogation of single nucleotide polymorphisms using the mismatch amplification mutation assay and capillary electrophoresis
AU - Price, Erin
AU - Matthews, Molly
AU - Beaudry, Jodi
AU - Allred, Jonathan
AU - Schupp, James
AU - Birdsell, Dawn
AU - Pearson, Talima
AU - Keim, Paul S
PY - 2010/12
Y1 - 2010/12
N2 - The ability to characterize SNPs is an important aspect of many clinical diagnostic, genetic and evolutionary studies. Here, we designed a multiplexed SNP genotyping method to survey a large number of phylogenetically informative SNPs within the genome of the bacterium Bacillus anthracis. This novel method, CE universal tail mismatch amplification mutation assay (CUMA), allows for PCR multiplexing and automatic scoring of SNP genotypes, thus providing a rapid, economical and higher throughput alternative to more expensive SNP genotyping techniques. CUMA delivered accurate B. anthracis SNP genotyping results and, when multiplexed, saved reagent costs by more than 80% compared with TaqMan real‐time PCR. When real‐time PCR technology and instrumentation is unavailable or the reagents are cost‐prohibitive, CUMA is a powerful alternative for SNP genotyping.
AB - The ability to characterize SNPs is an important aspect of many clinical diagnostic, genetic and evolutionary studies. Here, we designed a multiplexed SNP genotyping method to survey a large number of phylogenetically informative SNPs within the genome of the bacterium Bacillus anthracis. This novel method, CE universal tail mismatch amplification mutation assay (CUMA), allows for PCR multiplexing and automatic scoring of SNP genotypes, thus providing a rapid, economical and higher throughput alternative to more expensive SNP genotyping techniques. CUMA delivered accurate B. anthracis SNP genotyping results and, when multiplexed, saved reagent costs by more than 80% compared with TaqMan real‐time PCR. When real‐time PCR technology and instrumentation is unavailable or the reagents are cost‐prohibitive, CUMA is a powerful alternative for SNP genotyping.
UR - http://www.scopus.com/inward/record.url?scp=78650029130&partnerID=8YFLogxK
U2 - 10.1002/elps.201000379
DO - 10.1002/elps.201000379
M3 - Article
VL - 31
SP - 3881
EP - 3888
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 23-24
ER -