Abstract
Original language | English |
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Pages (from-to) | 798-802 |
Number of pages | 5 |
Journal | Vaccine |
Volume | 28 |
Issue number | 3 |
DOIs | |
Publication status | Published - 8 Jan 2010 |
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Cross-neutralisation of Australian brown snake, taipan and death adder venoms by monovalent antibodies. / Isbister, Geoffrey; O'Leary, Margaret; Hagan, Jessica; Nichols, Kearney; Jacoby, Tamara; Davern, Kathleen; Hodgson, Wayne; Schneider, Jennifer.
In: Vaccine, Vol. 28, No. 3, 08.01.2010, p. 798-802.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Cross-neutralisation of Australian brown snake, taipan and death adder venoms by monovalent antibodies
AU - Isbister, Geoffrey
AU - O'Leary, Margaret
AU - Hagan, Jessica
AU - Nichols, Kearney
AU - Jacoby, Tamara
AU - Davern, Kathleen
AU - Hodgson, Wayne
AU - Schneider, Jennifer
PY - 2010/1/8
Y1 - 2010/1/8
N2 - An understanding of the cross-neutralisation of snake venoms by antibodies is important for snake antivenom development. We investigated the cross-neutralisation of brown snake (Pseudonaja textilis) venom, taipan (Oxyuranus scutellatus) venom and death adder (Acanthophis antarcticus) with commercial antivenoms and monovalent anti-snake IgG, using enzyme immunoassays, in vitro clotting and neurotoxicity assays. Each commercial antivenom bound all three venoms, and neutralised clotting activity of brown snake and taipan venoms and neurotoxicity of death adder venom. The 'in-house' monovalent anti-snake venom IgG raised against procoagulant brown snake and taipan venoms, did not neutralise the neurotoxic effects of death adder venom. However, they did cross-neutralise the procoagulant effects of both procoagulant venoms. This supports the idea of developing antivenoms against groups of snake toxins rather than individual snake venoms. Crown Copyright � 2009.
AB - An understanding of the cross-neutralisation of snake venoms by antibodies is important for snake antivenom development. We investigated the cross-neutralisation of brown snake (Pseudonaja textilis) venom, taipan (Oxyuranus scutellatus) venom and death adder (Acanthophis antarcticus) with commercial antivenoms and monovalent anti-snake IgG, using enzyme immunoassays, in vitro clotting and neurotoxicity assays. Each commercial antivenom bound all three venoms, and neutralised clotting activity of brown snake and taipan venoms and neurotoxicity of death adder venom. The 'in-house' monovalent anti-snake venom IgG raised against procoagulant brown snake and taipan venoms, did not neutralise the neurotoxic effects of death adder venom. However, they did cross-neutralise the procoagulant effects of both procoagulant venoms. This supports the idea of developing antivenoms against groups of snake toxins rather than individual snake venoms. Crown Copyright � 2009.
KW - immunoglobulin G antibody
KW - snake venom
KW - snake venom antiserum
KW - acanthophis antarcticus
KW - animal experiment
KW - antibody titer
KW - article
KW - blood clotting
KW - controlled study
KW - drug mechanism
KW - enzyme immunoassay
KW - male
KW - neurotoxicity
KW - nonhuman
KW - oxyuranus scutellatus
KW - priority journal
KW - Pseudonaja textilis
KW - snake
KW - Animals
KW - Antibodies, Neutralizing
KW - Antivenins
KW - Australia
KW - Blood Coagulation
KW - Cross Reactions
KW - Elapidae
KW - Enzyme-Linked Immunosorbent Assay
KW - Immunoglobulin G
KW - Neurons
KW - Rabbits
KW - Venoms
U2 - 10.1016/j.vaccine.2009.10.055
DO - 10.1016/j.vaccine.2009.10.055
M3 - Article
VL - 28
SP - 798
EP - 802
JO - Vaccine
JF - Vaccine
SN - 0264-410X
IS - 3
ER -