Crusted scabies is associated with increased IL-17 secretion by skin T cells

X. Liu, S. F. Walton, H. C. Murray, M. King, A. Kelly, D. C. Holt, B. J. Currie, J. S. Mccarthy, K. E. Mounsey

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Scabies is an ectoparasitic infestation by the mite Sarcoptes scabiei. Although commonly self-limiting, a fraction of patients develop severely debilitating crusted scabies. The immune mechanisms underlying the development of crusted scabies are unclear, and undertaking longitudinal infection studies in humans is difficult. We utilized a porcine model to compare cellular immune responses in peripheral blood and skin of pigs with different clinical manifestations of scabies (n = 12), and in uninfected controls (n = 6). Although clinical symptoms were not evident until at least 4 weeks post-infestation, the numbers of peripheral IFNγ-secreting CD4+ T cells and γδ T cells increased in infected pigs from week 1 post-infestation. γδ T cells remained increased in the blood at week 15 post-infestation. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, γδ T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. Given the potential of anti-IL-17 immunotherapy demonstrated for other inflammatory skin diseases, this study may provide a novel therapeutic avenue for patients with recurrent crusted scabies.

Original languageEnglish
Pages (from-to)594-604
Number of pages11
JournalParasite Immunology
Volume36
Issue number11
DOIs
Publication statusPublished - 1 Nov 2014

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Scabies
Interleukin-17
T-Lymphocytes
Skin
Swine
Ectoparasitic Infestations
Sarcoptes scabiei
Mites
Skin Diseases
Cellular Immunity
Immunotherapy
Longitudinal Studies
Cell Count

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Liu, X., Walton, S. F., Murray, H. C., King, M., Kelly, A., Holt, D. C., ... Mounsey, K. E. (2014). Crusted scabies is associated with increased IL-17 secretion by skin T cells. Parasite Immunology, 36(11), 594-604. https://doi.org/10.1111/pim.12129
Liu, X. ; Walton, S. F. ; Murray, H. C. ; King, M. ; Kelly, A. ; Holt, D. C. ; Currie, B. J. ; Mccarthy, J. S. ; Mounsey, K. E. / Crusted scabies is associated with increased IL-17 secretion by skin T cells. In: Parasite Immunology. 2014 ; Vol. 36, No. 11. pp. 594-604.
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abstract = "Scabies is an ectoparasitic infestation by the mite Sarcoptes scabiei. Although commonly self-limiting, a fraction of patients develop severely debilitating crusted scabies. The immune mechanisms underlying the development of crusted scabies are unclear, and undertaking longitudinal infection studies in humans is difficult. We utilized a porcine model to compare cellular immune responses in peripheral blood and skin of pigs with different clinical manifestations of scabies (n = 12), and in uninfected controls (n = 6). Although clinical symptoms were not evident until at least 4 weeks post-infestation, the numbers of peripheral IFNγ-secreting CD4+ T cells and γδ T cells increased in infected pigs from week 1 post-infestation. γδ T cells remained increased in the blood at week 15 post-infestation. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, γδ T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. Given the potential of anti-IL-17 immunotherapy demonstrated for other inflammatory skin diseases, this study may provide a novel therapeutic avenue for patients with recurrent crusted scabies.",
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Liu, X, Walton, SF, Murray, HC, King, M, Kelly, A, Holt, DC, Currie, BJ, Mccarthy, JS & Mounsey, KE 2014, 'Crusted scabies is associated with increased IL-17 secretion by skin T cells', Parasite Immunology, vol. 36, no. 11, pp. 594-604. https://doi.org/10.1111/pim.12129

Crusted scabies is associated with increased IL-17 secretion by skin T cells. / Liu, X.; Walton, S. F.; Murray, H. C.; King, M.; Kelly, A.; Holt, D. C.; Currie, B. J.; Mccarthy, J. S.; Mounsey, K. E.

In: Parasite Immunology, Vol. 36, No. 11, 01.11.2014, p. 594-604.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Liu, X.

AU - Walton, S. F.

AU - Murray, H. C.

AU - King, M.

AU - Kelly, A.

AU - Holt, D. C.

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AU - Mccarthy, J. S.

AU - Mounsey, K. E.

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AB - Scabies is an ectoparasitic infestation by the mite Sarcoptes scabiei. Although commonly self-limiting, a fraction of patients develop severely debilitating crusted scabies. The immune mechanisms underlying the development of crusted scabies are unclear, and undertaking longitudinal infection studies in humans is difficult. We utilized a porcine model to compare cellular immune responses in peripheral blood and skin of pigs with different clinical manifestations of scabies (n = 12), and in uninfected controls (n = 6). Although clinical symptoms were not evident until at least 4 weeks post-infestation, the numbers of peripheral IFNγ-secreting CD4+ T cells and γδ T cells increased in infected pigs from week 1 post-infestation. γδ T cells remained increased in the blood at week 15 post-infestation. At week 15, skin cell infiltrates from pigs with crusted scabies had significantly higher CD8+ T cell, γδ T cell and IL-17+ cell numbers than those with ordinary scabies. Peripheral IL-17 levels were not increased, suggesting that localized skin IL-17-secreting T cells may play a critical role in the pathogenesis of crusted scabies development. Given the potential of anti-IL-17 immunotherapy demonstrated for other inflammatory skin diseases, this study may provide a novel therapeutic avenue for patients with recurrent crusted scabies.

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KW - parasitology

KW - pathology

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KW - randomization

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KW - swine

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KW - Random Allocation

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KW - Skin

KW - Sus scrofa

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Liu X, Walton SF, Murray HC, King M, Kelly A, Holt DC et al. Crusted scabies is associated with increased IL-17 secretion by skin T cells. Parasite Immunology. 2014 Nov 1;36(11):594-604. https://doi.org/10.1111/pim.12129