Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease

L Pilkington, Karen Gibb, G Gurr, M Fletcher, A Nikandrow, Elizabeth J Elliot, R Van de Ven, D Read

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.
    Original languageEnglish
    Pages (from-to)754-762
    Number of pages9
    JournalPlant Pathology
    Volume52
    Issue number6
    Publication statusPublished - 2003

    Fingerprint

    Phytoplasma
    Medicago sativa
    alfalfa
    signs and symptoms (plants)
    polymerase chain reaction
    Faba bean phyllody phytoplasma
    Restriction Fragment Length Polymorphisms
    Polymerase Chain Reaction
    16SrII (Peanut WB group)
    Tomato big bud phytoplasma
    restriction fragment length polymorphism
    Phloem
    Vicia faba
    Intergenic DNA
    DNA Primers
    DNA primers
    Enzymes
    Lycopersicon esculentum
    Phylogeny
    enzymes

    Cite this

    Pilkington, L., Gibb, K., Gurr, G., Fletcher, M., Nikandrow, A., Elliot, E. J., ... Read, D. (2003). Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease. Plant Pathology, 52(6), 754-762.
    Pilkington, L ; Gibb, Karen ; Gurr, G ; Fletcher, M ; Nikandrow, A ; Elliot, Elizabeth J ; Van de Ven, R ; Read, D. / Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease. In: Plant Pathology. 2003 ; Vol. 52, No. 6. pp. 754-762.
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    abstract = "Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.",
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    Pilkington, L, Gibb, K, Gurr, G, Fletcher, M, Nikandrow, A, Elliot, EJ, Van de Ven, R & Read, D 2003, 'Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease', Plant Pathology, vol. 52, no. 6, pp. 754-762.

    Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease. / Pilkington, L; Gibb, Karen; Gurr, G; Fletcher, M; Nikandrow, A; Elliot, Elizabeth J; Van de Ven, R; Read, D.

    In: Plant Pathology, Vol. 52, No. 6, 2003, p. 754-762.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

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    AU - Pilkington, L

    AU - Gibb, Karen

    AU - Gurr, G

    AU - Fletcher, M

    AU - Nikandrow, A

    AU - Elliot, Elizabeth J

    AU - Van de Ven, R

    AU - Read, D

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    AB - Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lucerne plants with and without symptoms. Identical restriction fragment length polymorphism (RFLP) enzyme profiles were obtained for PCR products amplified from 10 yellows-affected lucerne samples. RFLP profiles obtained for four restriction enzymes were different from those of the tomato big bud (TBB) phytoplasma. ALuY phytoplasma PCR products were sequenced to determine phylogeny and were found to fall within the faba bean phyllody phytoplasma group, or phytoplasma group 16srII. Transmission electron microscopy revealed phytoplasmas in the phloem of yellows-affected plant samples, but not in symptomless plant samples. Fungal, bacterial and viral agents in the aetiology of Australian lucerne yellows were ruled out.

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    M3 - Article

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    SN - 0032-0862

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    Pilkington L, Gibb K, Gurr G, Fletcher M, Nikandrow A, Elliot EJ et al. Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease. Plant Pathology. 2003;52(6):754-762.