TY - JOUR
T1 - Development of 16 microsatellite loci for the endangered sandhill dunnart (Sminthopsis psammophila)
AU - McLean, Amanda L.
AU - Cooper, Steven J B
AU - Lancaster, Melanie L.
AU - Carthew, Susan M.
PY - 2014/6
Y1 - 2014/6
N2 - Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individual S. psammophila from two populations in South Australia. Three loci deviated from Hardy-Weinberg equilibrium, possibly due to null alleles. No evidence of linkage disequilibrium was detected. We recommend a final panel of 17 loci for future genetic analyses of S. psammophila, which will aid in conservation management of this poorly understood and nationally endangered marsupial.
AB - Sixteen polymorphic microsatellite loci were developed using a 454 sequencing approach, and three loci, developed for Sminthopsis douglasi, were optimized for the endangered sandhill dunnart, Sminthopsis psammophila. Nineteen loci (16 S. psammophila and three S. douglasi) were tested on 38 individual S. psammophila from two populations in South Australia. Three loci deviated from Hardy-Weinberg equilibrium, possibly due to null alleles. No evidence of linkage disequilibrium was detected. We recommend a final panel of 17 loci for future genetic analyses of S. psammophila, which will aid in conservation management of this poorly understood and nationally endangered marsupial.
KW - 454 sequencing
KW - Endangered marsupial
KW - Microsatellites
KW - Sminthopsis psammophila
UR - http://www.scopus.com/inward/record.url?scp=84900790006&partnerID=8YFLogxK
U2 - 10.1007/s12686-013-0084-5
DO - 10.1007/s12686-013-0084-5
M3 - Letter
VL - 6
SP - 323
EP - 326
JO - Conservation Genetics Resources
JF - Conservation Genetics Resources
SN - 1877-7252
IS - 2
ER -