DNA barcoding and phylogenetic analysis of midges belonging to Culicoides (Diptera: Ceratopogonidae) subgenus Hoffmania in Yunnan, China

Ying Liang Duan; Glenn Bellis; Zhen Xing Yang; Zhan Hong Li; Bing Gang Liu; Le Li

doi:10.1016/j.aspen.2022.101989

DNA barcoding and phylogenetic analysis of midges belonging to Culicoides (Diptera: Ceratopogonidae) subgenus Hoffmania in Yunnan, China

Ying Liang Duan, Glenn Bellis, Zhen Xing Yang, Zhan Hong Li, Bing Gang Liu, Le Li

Research Institute for Environment and Livelihoods

Research output: Contribution to journal › Article › peer-review

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Abstract

DNA barcodes obtained from cytochrome c oxidase subunit 1 (cox1) offer a fast and easy way to identify a range of biological organisms. Culicoides (Diptera: Ceratopogonidae) are a group of small, blood sucking midges whose species are the vectors for some arboviruses, such as bluetongue virus, African horse sickness virus, epizootic hemorrhagic disease virus and equine encephalosis virus. Identification of these small insects is difficult so constructing DNA barcode libraries for species present in certain areas is helpful to clarify the taxonomy and assist non-specialist workers to identify species. In this study, we analysed specimens belonging to C. subgenus Hoffmania collected from 12 towns of Yunnan Province, China. Specimens were identified by morphology and processed to construct DNA barcodes. A total of 185 specimens referable to 6 morphological species were processed for cox1 and 28S rRNA sequencing. The resulting 185 cox1 sequences were assigned to 13 barcode index numbers (BINs) which include 9 novel BINs. Molecular and morphological evidence was used to support the transfer of 4 species previously assigned to C. subg. Avaritia into C. subg. Hoffmania. Molecular analysis revealed the presence of 7 potential cryptic species within C. innoxius, three within C. liui and two within C. insignipennis.

Original language	English
Article number	101989
Pages (from-to)	1-9
Number of pages	9
Journal	Journal of Asia Pacific Entomology
Volume	25
Issue number	4
DOIs	https://doi.org/10.1016/j.aspen.2022.101989
Publication status	Published - Dec 2022

Bibliographical note

Funding Information:
This work was supported by grants from the National Natural Science Foundation of China ( 32160846 ) and the Foreign Experts Project of Yunnan Province (YNZ2019002 and YNZ2020015 ). The Australian Government Department of Agriculture, Water and the Environment supported the collaboration of GAB, LL and YLD. The authors are thankful to Professor Guo-Ping Liu (Center for Disease Control and Prevention of Northern Theater Command, Shenyang, China) for advice on species identification.

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@article{0a389e4568304023978c30f478a561f1,

title = "DNA barcoding and phylogenetic analysis of midges belonging to Culicoides (Diptera: Ceratopogonidae) subgenus Hoffmania in Yunnan, China",

abstract = "DNA barcodes obtained from cytochrome c oxidase subunit 1 (cox1) offer a fast and easy way to identify a range of biological organisms. Culicoides (Diptera: Ceratopogonidae) are a group of small, blood sucking midges whose species are the vectors for some arboviruses, such as bluetongue virus, African horse sickness virus, epizootic hemorrhagic disease virus and equine encephalosis virus. Identification of these small insects is difficult so constructing DNA barcode libraries for species present in certain areas is helpful to clarify the taxonomy and assist non-specialist workers to identify species. In this study, we analysed specimens belonging to C. subgenus Hoffmania collected from 12 towns of Yunnan Province, China. Specimens were identified by morphology and processed to construct DNA barcodes. A total of 185 specimens referable to 6 morphological species were processed for cox1 and 28S rRNA sequencing. The resulting 185 cox1 sequences were assigned to 13 barcode index numbers (BINs) which include 9 novel BINs. Molecular and morphological evidence was used to support the transfer of 4 species previously assigned to C. subg. Avaritia into C. subg. Hoffmania. Molecular analysis revealed the presence of 7 potential cryptic species within C. innoxius, three within C. liui and two within C. insignipennis.",

keywords = "Culicoides, Hoffmania, C. innoxius, Cox1, 28S rRNA, DNA barcode",

author = "Duan, {Ying Liang} and Glenn Bellis and Yang, {Zhen Xing} and Li, {Zhan Hong} and Liu, {Bing Gang} and Le Li",

note = "Funding Information: This work was supported by grants from the National Natural Science Foundation of China ( 32160846 ) and the Foreign Experts Project of Yunnan Province (YNZ2019002 and YNZ2020015 ). The Australian Government Department of Agriculture, Water and the Environment supported the collaboration of GAB, LL and YLD. The authors are thankful to Professor Guo-Ping Liu (Center for Disease Control and Prevention of Northern Theater Command, Shenyang, China) for advice on species identification. ",

year = "2022",

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doi = "10.1016/j.aspen.2022.101989",

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AU - Yang, Zhen Xing

AU - Li, Zhan Hong

AU - Liu, Bing Gang

AU - Li, Le

N1 - Funding Information: This work was supported by grants from the National Natural Science Foundation of China ( 32160846 ) and the Foreign Experts Project of Yunnan Province (YNZ2019002 and YNZ2020015 ). The Australian Government Department of Agriculture, Water and the Environment supported the collaboration of GAB, LL and YLD. The authors are thankful to Professor Guo-Ping Liu (Center for Disease Control and Prevention of Northern Theater Command, Shenyang, China) for advice on species identification.

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N2 - DNA barcodes obtained from cytochrome c oxidase subunit 1 (cox1) offer a fast and easy way to identify a range of biological organisms. Culicoides (Diptera: Ceratopogonidae) are a group of small, blood sucking midges whose species are the vectors for some arboviruses, such as bluetongue virus, African horse sickness virus, epizootic hemorrhagic disease virus and equine encephalosis virus. Identification of these small insects is difficult so constructing DNA barcode libraries for species present in certain areas is helpful to clarify the taxonomy and assist non-specialist workers to identify species. In this study, we analysed specimens belonging to C. subgenus Hoffmania collected from 12 towns of Yunnan Province, China. Specimens were identified by morphology and processed to construct DNA barcodes. A total of 185 specimens referable to 6 morphological species were processed for cox1 and 28S rRNA sequencing. The resulting 185 cox1 sequences were assigned to 13 barcode index numbers (BINs) which include 9 novel BINs. Molecular and morphological evidence was used to support the transfer of 4 species previously assigned to C. subg. Avaritia into C. subg. Hoffmania. Molecular analysis revealed the presence of 7 potential cryptic species within C. innoxius, three within C. liui and two within C. insignipennis.

AB - DNA barcodes obtained from cytochrome c oxidase subunit 1 (cox1) offer a fast and easy way to identify a range of biological organisms. Culicoides (Diptera: Ceratopogonidae) are a group of small, blood sucking midges whose species are the vectors for some arboviruses, such as bluetongue virus, African horse sickness virus, epizootic hemorrhagic disease virus and equine encephalosis virus. Identification of these small insects is difficult so constructing DNA barcode libraries for species present in certain areas is helpful to clarify the taxonomy and assist non-specialist workers to identify species. In this study, we analysed specimens belonging to C. subgenus Hoffmania collected from 12 towns of Yunnan Province, China. Specimens were identified by morphology and processed to construct DNA barcodes. A total of 185 specimens referable to 6 morphological species were processed for cox1 and 28S rRNA sequencing. The resulting 185 cox1 sequences were assigned to 13 barcode index numbers (BINs) which include 9 novel BINs. Molecular and morphological evidence was used to support the transfer of 4 species previously assigned to C. subg. Avaritia into C. subg. Hoffmania. Molecular analysis revealed the presence of 7 potential cryptic species within C. innoxius, three within C. liui and two within C. insignipennis.

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JO - Journal of Asia Pacific Entomology

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M1 - 101989

ER -

DNA barcoding and phylogenetic analysis of midges belonging to Culicoides (Diptera: Ceratopogonidae) subgenus Hoffmania in Yunnan, China

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