DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix

Jacklyn Ng; Deborah Holt; Patiyan Andersson; Philip Giffard

doi:10.1373/clinchem.2013.215582

DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix

Jacklyn Ng, Deborah Holt, Patiyan Andersson, Philip Giffard

Global and Tropical Health

Research output: Contribution to journal › Letter › peer-review

4 Citations (Scopus)

Abstract

[No abstract available]

Original language	English
Pages (from-to)	414-416
Number of pages	3
Journal	Clinical Chemistry
Volume	60
Issue number	2
DOIs	https://doi.org/10.1373/clinchem.2013.215582
Publication status	Published - 2014

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10.1373/clinchem.2013.215582

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@article{7bafa3005efd430b8b2dcf3eda4cff89,

title = "DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix",

abstract = "[No abstract available]",

keywords = "cytosine, DNA, DNA fragment, glycerol, guanine, magnesium chloride, magnesium ion, bacterial DNA, primer DNA, allele, DNA base composition, DNA binding, DNA strand, gene amplification, high resolution melting analysis, letter, limit of detection, melting point, multilocus sequence typing, polymerase chain reaction system, Staphylococcus aureus, stoichiometry, temperature, chemistry, DNA denaturation, genetics, heat, molecular genetics, nucleotide sequence, polymerase chain reaction, procedures, transition temperature, Base Sequence, DNA Primers, DNA, Bacterial, Hot Temperature, Molecular Sequence Data, Nucleic Acid Denaturation, Polymerase Chain Reaction, Transition Temperature",

author = "Jacklyn Ng and Deborah Holt and Patiyan Andersson and Philip Giffard",

year = "2014",

doi = "10.1373/clinchem.2013.215582",

language = "English",

volume = "60",

pages = "414--416",

journal = "Clinical Chemistry",

issn = "0009-9147",

publisher = "American Association for Clinical Chemistry",

number = "2",

}

TY - JOUR

T1 - DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix

AU - Ng, Jacklyn

AU - Holt, Deborah

AU - Andersson, Patiyan

AU - Giffard, Philip

PY - 2014

Y1 - 2014

N2 - [No abstract available]

AB - [No abstract available]

KW - cytosine

KW - DNA

KW - DNA fragment

KW - glycerol

KW - guanine

KW - magnesium chloride

KW - magnesium ion

KW - bacterial DNA

KW - primer DNA

KW - allele

KW - DNA base composition

KW - DNA binding

KW - DNA strand

KW - gene amplification

KW - high resolution melting analysis

KW - letter

KW - limit of detection

KW - melting point

KW - multilocus sequence typing

KW - polymerase chain reaction system

KW - Staphylococcus aureus

KW - stoichiometry

KW - temperature

KW - chemistry

KW - DNA denaturation

KW - genetics

KW - heat

KW - molecular genetics

KW - nucleotide sequence

KW - polymerase chain reaction

KW - procedures

KW - transition temperature

KW - Base Sequence

KW - DNA Primers

KW - DNA, Bacterial

KW - Hot Temperature

KW - Molecular Sequence Data

KW - Nucleic Acid Denaturation

KW - Polymerase Chain Reaction

KW - Transition Temperature

UR - http://www.scopus.com/inward/record.url?scp=84893457532&partnerID=8YFLogxK

U2 - 10.1373/clinchem.2013.215582

DO - 10.1373/clinchem.2013.215582

M3 - Letter

C2 - PubMed:24323978

SN - 0009-9147

VL - 60

SP - 414

EP - 416

JO - Clinical Chemistry

JF - Clinical Chemistry

IS - 2

ER -

DNA concentration can specify DNA melting point in a high-resolution melting analysis master mix

Abstract

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