Do combined upper airway cultures identify lower airway infections in children with chronic cough?

Kim Hare, Anne Chang, Heidi Smith-Vaughan, Paul Bauert, Brian Spain, Jemima Beissbarth, Keith Grimwood

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Background: Obtaining lower airway specimens is important for guiding therapy in chronic lung infection but is difficult in young children unable to expectorate. While culture‐based studies have assessed the diagnostic accuracy of nasopharyngeal or oropharyngeal specimens for identifying lower airway infection, none have used both together. We compared respiratory bacterial pathogens cultured from nasopharyngeal and oropharyngeal swabs with bronchoalveolar lavage (BAL) cultures as the “gold standard” to better inform the diagnosis of lower airway infection in children with chronic wet cough.

    Methods: Nasopharyngeal and oropharyngeal swabs and BAL fluid specimens were collected concurrently from consecutive children undergoing flexible bronchoscopy for chronic cough and cultured for bacterial pathogens.

    Results: In cultures from 309 children (median age, 2.3 years) with chronic endobronchial suppuration, all main pathogens detected (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) were more prevalent in nasopharyngeal than oropharyngeal swabs (37%, 34%, and 23% vs 21%, 6.2%, and 3.2%, respectively). Positive and negative predictive values for lower airway infection by any of these three pathogens were 63% (95% confidence interval [95% CI] 55, 70) and 85% (95% CI, 78, 91) for nasopharyngeal swabs, 65% (95% CI, 54, 75), and 66% (95% CI, 59, 72) for oropharyngeal swabs, and 61% (95% CI, 54,68), and 88% (95% CI, 81, 93) for both swabs, respectively.

    Conclusions: Neither nasopharyngeal nor oropharyngeal swabs, alone or in combination, reliably predicted lower airway infection in children with chronic wet cough. Although upper airway specimens may be useful for bacterial carriage studies and monitoring antimicrobial resistance, their clinical utility in pediatric chronic lung disorders of endobronchial suppuration is limited.
    Original languageEnglish
    Pages (from-to)907-913
    Number of pages7
    JournalPediatric Pulmonology
    Volume54
    Issue number6
    Early online date21 Apr 2019
    DOIs
    Publication statusPublished - Jun 2019

    Fingerprint

    Cough
    Confidence Intervals
    Infection
    Suppuration
    Moraxella (Branhamella) catarrhalis
    Lung
    Bronchoalveolar Lavage Fluid
    Haemophilus influenzae
    Bronchoscopy
    Bronchoalveolar Lavage
    Streptococcus pneumoniae
    Pediatrics

    Cite this

    Hare, Kim ; Chang, Anne ; Smith-Vaughan, Heidi ; Bauert, Paul ; Spain, Brian ; Beissbarth, Jemima ; Grimwood, Keith. / Do combined upper airway cultures identify lower airway infections in children with chronic cough?. In: Pediatric Pulmonology. 2019 ; Vol. 54, No. 6. pp. 907-913.
    @article{8215d8b6d3104c74be6bdb76404f6349,
    title = "Do combined upper airway cultures identify lower airway infections in children with chronic cough?",
    abstract = "Background: Obtaining lower airway specimens is important for guiding therapy in chronic lung infection but is difficult in young children unable to expectorate. While culture‐based studies have assessed the diagnostic accuracy of nasopharyngeal or oropharyngeal specimens for identifying lower airway infection, none have used both together. We compared respiratory bacterial pathogens cultured from nasopharyngeal and oropharyngeal swabs with bronchoalveolar lavage (BAL) cultures as the “gold standard” to better inform the diagnosis of lower airway infection in children with chronic wet cough.Methods: Nasopharyngeal and oropharyngeal swabs and BAL fluid specimens were collected concurrently from consecutive children undergoing flexible bronchoscopy for chronic cough and cultured for bacterial pathogens.Results: In cultures from 309 children (median age, 2.3 years) with chronic endobronchial suppuration, all main pathogens detected (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) were more prevalent in nasopharyngeal than oropharyngeal swabs (37{\%}, 34{\%}, and 23{\%} vs 21{\%}, 6.2{\%}, and 3.2{\%}, respectively). Positive and negative predictive values for lower airway infection by any of these three pathogens were 63{\%} (95{\%} confidence interval [95{\%} CI] 55, 70) and 85{\%} (95{\%} CI, 78, 91) for nasopharyngeal swabs, 65{\%} (95{\%} CI, 54, 75), and 66{\%} (95{\%} CI, 59, 72) for oropharyngeal swabs, and 61{\%} (95{\%} CI, 54,68), and 88{\%} (95{\%} CI, 81, 93) for both swabs, respectively.Conclusions: Neither nasopharyngeal nor oropharyngeal swabs, alone or in combination, reliably predicted lower airway infection in children with chronic wet cough. Although upper airway specimens may be useful for bacterial carriage studies and monitoring antimicrobial resistance, their clinical utility in pediatric chronic lung disorders of endobronchial suppuration is limited.",
    author = "Kim Hare and Anne Chang and Heidi Smith-Vaughan and Paul Bauert and Brian Spain and Jemima Beissbarth and Keith Grimwood",
    year = "2019",
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    Do combined upper airway cultures identify lower airway infections in children with chronic cough? / Hare, Kim; Chang, Anne; Smith-Vaughan, Heidi; Bauert, Paul; Spain, Brian; Beissbarth, Jemima; Grimwood, Keith.

    In: Pediatric Pulmonology, Vol. 54, No. 6, 06.2019, p. 907-913.

    Research output: Contribution to journalArticleResearchpeer-review

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    T1 - Do combined upper airway cultures identify lower airway infections in children with chronic cough?

    AU - Hare, Kim

    AU - Chang, Anne

    AU - Smith-Vaughan, Heidi

    AU - Bauert, Paul

    AU - Spain, Brian

    AU - Beissbarth, Jemima

    AU - Grimwood, Keith

    PY - 2019/6

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    N2 - Background: Obtaining lower airway specimens is important for guiding therapy in chronic lung infection but is difficult in young children unable to expectorate. While culture‐based studies have assessed the diagnostic accuracy of nasopharyngeal or oropharyngeal specimens for identifying lower airway infection, none have used both together. We compared respiratory bacterial pathogens cultured from nasopharyngeal and oropharyngeal swabs with bronchoalveolar lavage (BAL) cultures as the “gold standard” to better inform the diagnosis of lower airway infection in children with chronic wet cough.Methods: Nasopharyngeal and oropharyngeal swabs and BAL fluid specimens were collected concurrently from consecutive children undergoing flexible bronchoscopy for chronic cough and cultured for bacterial pathogens.Results: In cultures from 309 children (median age, 2.3 years) with chronic endobronchial suppuration, all main pathogens detected (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) were more prevalent in nasopharyngeal than oropharyngeal swabs (37%, 34%, and 23% vs 21%, 6.2%, and 3.2%, respectively). Positive and negative predictive values for lower airway infection by any of these three pathogens were 63% (95% confidence interval [95% CI] 55, 70) and 85% (95% CI, 78, 91) for nasopharyngeal swabs, 65% (95% CI, 54, 75), and 66% (95% CI, 59, 72) for oropharyngeal swabs, and 61% (95% CI, 54,68), and 88% (95% CI, 81, 93) for both swabs, respectively.Conclusions: Neither nasopharyngeal nor oropharyngeal swabs, alone or in combination, reliably predicted lower airway infection in children with chronic wet cough. Although upper airway specimens may be useful for bacterial carriage studies and monitoring antimicrobial resistance, their clinical utility in pediatric chronic lung disorders of endobronchial suppuration is limited.

    AB - Background: Obtaining lower airway specimens is important for guiding therapy in chronic lung infection but is difficult in young children unable to expectorate. While culture‐based studies have assessed the diagnostic accuracy of nasopharyngeal or oropharyngeal specimens for identifying lower airway infection, none have used both together. We compared respiratory bacterial pathogens cultured from nasopharyngeal and oropharyngeal swabs with bronchoalveolar lavage (BAL) cultures as the “gold standard” to better inform the diagnosis of lower airway infection in children with chronic wet cough.Methods: Nasopharyngeal and oropharyngeal swabs and BAL fluid specimens were collected concurrently from consecutive children undergoing flexible bronchoscopy for chronic cough and cultured for bacterial pathogens.Results: In cultures from 309 children (median age, 2.3 years) with chronic endobronchial suppuration, all main pathogens detected (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) were more prevalent in nasopharyngeal than oropharyngeal swabs (37%, 34%, and 23% vs 21%, 6.2%, and 3.2%, respectively). Positive and negative predictive values for lower airway infection by any of these three pathogens were 63% (95% confidence interval [95% CI] 55, 70) and 85% (95% CI, 78, 91) for nasopharyngeal swabs, 65% (95% CI, 54, 75), and 66% (95% CI, 59, 72) for oropharyngeal swabs, and 61% (95% CI, 54,68), and 88% (95% CI, 81, 93) for both swabs, respectively.Conclusions: Neither nasopharyngeal nor oropharyngeal swabs, alone or in combination, reliably predicted lower airway infection in children with chronic wet cough. Although upper airway specimens may be useful for bacterial carriage studies and monitoring antimicrobial resistance, their clinical utility in pediatric chronic lung disorders of endobronchial suppuration is limited.

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    DO - 10.1002/ppul.24336

    M3 - Article

    VL - 54

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    JF - Pediatric Pulmonology

    SN - 1099-0496

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