Sulphate reducing bacteria (SRB) can be found in alkaline environments. Due to their metabolite products such as hydrogen sulphide, the corrosion behaviour of materials in alkaline environments may be affected by the presence of SRB. This study focuses on the investigation of corrosion behaviour of duplex stainless steel DSS 2205 in nutrient rich artificial seawater containing SRB species, Desulfovibrio vulgaris, at different alkaline conditions with pH range from 7 to 10. The open circuit potential value (OCP), sulphide level and pH were recorded daily. Confocal laser scanning microscopy (CLSM) was used to study the adhesion of SRB on the DSS 2205 surface. Electrochemical impedance spectroscopy (EIS) was used to study the properties of the biofilm. Potentiodynamic polarization was used to study the corrosion behaviour of material. Inductively coupled plasma mass was used to measure the concentration of cations Fe, Ni, Mo, Mn in the experimental solution after 28 days. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were used for surface analysis. The results showed that D. vulgaris are active in an alkaline environment with pH 7-9. However, at pH 10, D. vulgaris activity exhibited an 8-day lag. The corrosion rate of DSS 2205 at pH 9 was higher than at other pH environments due to a higher dissolved concentration of hydrogen sulphide.