Exome sequencing reveals primary immunodeficiencies in children with community-acquired Pseudomonas aeruginosa sepsis

Samira Asgari, Paul J. McLaren, Jane Peake, Melanie Wong, Richard Wong, Istvan Bartha, Joshua R. Francis, Katia Abarca, Kyra A. Gelderman, Philipp Agyeman, Christoph Aebi, Christoph Berger, Jacques Fellay, Luregn J. Schlapbach, Klara Posfay-Barbe, Eric Giannoni, Bendicht P. Wagner, Ulrich Heininger, Gabriel Konetzny, Alex DonasMartin Stocker, Antonio Leone, Paul Hasters, Anita Niederer-Loher, Christian Kahlert, Walter Baer, Christa Relly, The Swiss Pediatric Sepsis Study

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    Abstract

    One out of three pediatric sepsis deaths in high income countries occur in previously healthy children. Primary immunodeficiencies (PIDs) have been postulated to underlie fulminant sepsis, but this concept remains to be confirmed in clinical practice. Pseudomonas aeruginosa (P. aeruginosa) is a common bacterium mostly associated with health care-related infections in immunocompromised individuals. However, in rare cases, it can cause sepsis in previously healthy children. We used exome sequencing and bioinformatic analysis to systematically search for genetic factors underpinning severe P. aeruginosa infection in the pediatric population. We collected blood samples from 11 previously healthy children, with no family history of immunodeficiency, who presented with severe sepsis due to community-acquired P. aeruginosa bacteremia. Genomic DNA was extracted from blood or tissue samples obtained intravitam or postmortem. We obtained high-coverage exome sequencing data and searched for rare loss-of-function variants. After rigorous filtrations, 12 potentially causal variants were identified. Two out of eight (25%) fatal cases were found to carry novel pathogenic variants in PID genes, including BTK and DNMT3B. This study demonstrates that exome sequencing allows to identify rare, deleterious human genetic variants responsible for fulminant sepsis in apparently healthy children. Diagnosing PIDs in such patients is of high relevance to survivors and affected families. We propose that unusually severe and fatal sepsis cases in previously healthy children should be considered for exome/genome sequencing to search for underlying PIDs.

    Original languageEnglish
    Article number357
    Pages (from-to)1-11
    Number of pages11
    JournalFrontiers in Immunology
    Volume7
    DOIs
    Publication statusPublished - 20 Sep 2016

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    Asgari, S., McLaren, P. J., Peake, J., Wong, M., Wong, R., Bartha, I., Francis, J. R., Abarca, K., Gelderman, K. A., Agyeman, P., Aebi, C., Berger, C., Fellay, J., Schlapbach, L. J., Posfay-Barbe, K., Giannoni, E., Wagner, B. P., Heininger, U., Konetzny, G., ... The Swiss Pediatric Sepsis Study (2016). Exome sequencing reveals primary immunodeficiencies in children with community-acquired Pseudomonas aeruginosa sepsis. Frontiers in Immunology, 7, 1-11. [357]. https://doi.org/10.3389/fimmu.2016.00357