Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

Carina M. Hall; Anthony L. Baker; Jason W. Sahl; Mark Mayo; Holger C. Scholz; Mirjam Kaestli; James Schupp; Madison Martz; Erik W. Settles; Joseph D. Busch; Lindsay Sidak-Loftis; Astrid Thomas; Lisa Kreutzer; Enrico Georgi; Herbert P. Schweizer; Jeffrey M. Warner; Paul Keim; Bart J. Currie; David M. Wagner

doi:10.1128/AEM.01583-21

Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

Carina M. Hall, Anthony L. Baker, Jason W. Sahl, Mark Mayo, Holger C. Scholz, Mirjam Kaestli, James Schupp, Madison Martz, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Herbert P. Schweizer, Jeffrey M. Warner, Paul Keim, Bart J. Currie, David M. Wagner

Global and Tropical Health

Research output: Contribution to journal › Article › peer-review

Abstract

Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6T (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266T (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species currently within the Bpc.

Original language	English
Article number	e01583-21
Pages (from-to)	1-13
Number of pages	13
Journal	Applied and Environmental Microbiology
Volume	88
Issue number	1
DOIs	https://doi.org/10.1128/AEM.01583-21
Publication status	Published - Jan 2022

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Hall, C. M., Baker, A. L., Sahl, J. W., Mayo, M., Scholz, H. C., Kaestli, M., Schupp, J., Martz, M., Settles, E. W., Busch, J. D., Sidak-Loftis, L., Thomas, A., Kreutzer, L., Georgi, E., Schweizer, H. P., Warner, J. M., Keim, P., Currie, B. J., & Wagner, D. M. (2022). Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Applied and Environmental Microbiology, 88(1), 1-13. [e01583-21]. https://doi.org/10.1128/AEM.01583-21

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title = "Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.",

abstract = "Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6T (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266T (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species currently within the Bpc. ",

keywords = "BDU6T, Burkholderia, Burkholderia mayonis sp. nov., Burkholderia pseudomallei complex, Burkholderia savannae sp. nov., MSMB266T, Novel species",

author = "Hall, {Carina M.} and Baker, {Anthony L.} and Sahl, {Jason W.} and Mark Mayo and Scholz, {Holger C.} and Mirjam Kaestli and James Schupp and Madison Martz and Settles, {Erik W.} and Busch, {Joseph D.} and Lindsay Sidak-Loftis and Astrid Thomas and Lisa Kreutzer and Enrico Georgi and Schweizer, {Herbert P.} and Warner, {Jeffrey M.} and Paul Keim and Currie, {Bart J.} and Wagner, {David M.}",

note = "Funding Information: We declare no conflicts of interest. This work was funded by the DOD Defense Threat Reduction Agency (DTRA; HDTRA1-12-C-0066 and HDTRA1-17-1-0051), the Australian National Health and Medical Research Council, and the Australian Research Council. We are grateful to Aharon Oren for assistance with nomenclature. Publisher Copyright: {\textcopyright} 2022 American Society for Microbiology. All Rights Reserved. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.",

year = "2022",

month = jan,

doi = "10.1128/AEM.01583-21",

language = "English",

volume = "88",

pages = "1--13",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "1",

}

Hall, CM, Baker, AL, Sahl, JW, Mayo, M, Scholz, HC, Kaestli, M, Schupp, J, Martz, M, Settles, EW, Busch, JD, Sidak-Loftis, L, Thomas, A, Kreutzer, L, Georgi, E, Schweizer, HP, Warner, JM, Keim, P, Currie, BJ & Wagner, DM 2022, 'Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.', Applied and Environmental Microbiology, vol. 88, no. 1, e01583-21, pp. 1-13. https://doi.org/10.1128/AEM.01583-21

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T1 - Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species

T2 - Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

AU - Hall, Carina M.

AU - Baker, Anthony L.

AU - Sahl, Jason W.

AU - Mayo, Mark

AU - Scholz, Holger C.

AU - Kaestli, Mirjam

AU - Schupp, James

AU - Martz, Madison

AU - Settles, Erik W.

AU - Busch, Joseph D.

AU - Sidak-Loftis, Lindsay

AU - Thomas, Astrid

AU - Kreutzer, Lisa

AU - Georgi, Enrico

AU - Schweizer, Herbert P.

AU - Warner, Jeffrey M.

AU - Keim, Paul

AU - Currie, Bart J.

AU - Wagner, David M.

N1 - Funding Information: We declare no conflicts of interest. This work was funded by the DOD Defense Threat Reduction Agency (DTRA; HDTRA1-12-C-0066 and HDTRA1-17-1-0051), the Australian National Health and Medical Research Council, and the Australian Research Council. We are grateful to Aharon Oren for assistance with nomenclature. Publisher Copyright: © 2022 American Society for Microbiology. All Rights Reserved. Copyright: Copyright 2022 Elsevier B.V., All rights reserved.

PY - 2022/1

Y1 - 2022/1

N2 - Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6T (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266T (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species currently within the Bpc.

AB - Distinct Burkholderia strains were isolated from soil samples collected in tropical northern Australia (Northern Territory and the Torres Strait Islands, Queensland). Phylogenetic analysis of 16S rRNA and whole genome sequences revealed these strains were distinct from previously described Burkholderia species and assigned them to two novel clades within the B. pseudomallei complex (Bpc). Because average nucleotide identity and digital DNA-DNA hybridization calculations are consistent with these clades representing distinct species, we propose the names Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov. Strains assigned to B. mayonis sp. nov. include type strain BDU6T (=TSD-80; LMG 29941; ASM152374v2) and BDU8. Strains assigned to B. savannae sp. nov. include type strain MSMB266T (=TSD-82; LMG 29940; ASM152444v2), MSMB852, BDU18, and BDU19. Comparative genomics revealed unique coding regions for both putative species, including clusters of orthologous genes associated with phage. Type strains of both B. mayonis sp. nov. and B. savannae sp. nov. yielded biochemical profiles distinct from each other and from other species in the Bpc, and profiles also varied among strains within B. mayonis sp. nov. and B. savannae sp. nov. Matrix-assisted laser desorption ionization time-of-flight (MLST) analysis revealed a B. savannae sp. nov. cluster separate from other species, whereas B. mayonis sp. nov. strains did not form a distinct cluster. Neither B. mayonis sp. nov. nor B. savannae sp. nov. caused mortality in mice when delivered via the subcutaneous route. The addition of B. mayonis sp. nov. and B. savannae sp. nov. results in a total of eight species currently within the Bpc.

KW - BDU6T

KW - Burkholderia

KW - Burkholderia mayonis sp. nov.

KW - Burkholderia pseudomallei complex

KW - Burkholderia savannae sp. nov.

KW - MSMB266T

KW - Novel species

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SN - 0099-2240

VL - 88

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JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 1

M1 - e01583-21

ER -

Expanding the Burkholderia pseudomallei Complex with the Addition of Two Novel Species: Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov.

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