Extrachromosomal DNA isolated from tomato big bud and Candidatus Phytoplasma australiense phytoplasma strains

L Tran-nguyen, Karen Gibb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    The nucleotide sequences of two extrachromosomal elements from tomato big bud (TBB) and one extrachromosomal element from Candidatus Phytoplasma australiense (Ca. P. australiense) phytoplasmas were determined. Both TBB plasmids (3319 and 4092 bp) contained an open reading frame (?570 bp) with homology to the rolling circle replication initiator protein (Rep). This gene was shorter than the rep genes identified from other phytoplasma plasmids, geminiviruses and bacterial plasmids. Both TBB extrachromosomal DNAs (eDNAs) encoded a putative DNA primase (dnaG) gene, a chromosomal gene required for DNA replication and which contains the conserved topoisomerase/primase domain. We speculate that the replication mechanism for the TBB phytoplasma eDNA involves the dnaG gene instead of the rep gene. The Ca. P. australiense eDNA (3773 bp) was shown to be circular and contained four open reading frames. The rep gene was encoded on ORF 1 and had homology to both plasmid (pLS1) and geminivirus-like domains. � 2006 Elsevier Inc. All rights reserved.
    Original languageEnglish
    Pages (from-to)153-166
    Number of pages14
    JournalPlasmid
    Volume56
    Issue number3
    Publication statusPublished - 2006

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    Phytoplasma
    Lycopersicon esculentum
    DNA
    Plasmids
    Genes
    Geminiviridae
    DNA Primase
    Open Reading Frames
    DNA Replication

    Cite this

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    title = "Extrachromosomal DNA isolated from tomato big bud and Candidatus Phytoplasma australiense phytoplasma strains",
    abstract = "The nucleotide sequences of two extrachromosomal elements from tomato big bud (TBB) and one extrachromosomal element from Candidatus Phytoplasma australiense (Ca. P. australiense) phytoplasmas were determined. Both TBB plasmids (3319 and 4092 bp) contained an open reading frame (?570 bp) with homology to the rolling circle replication initiator protein (Rep). This gene was shorter than the rep genes identified from other phytoplasma plasmids, geminiviruses and bacterial plasmids. Both TBB extrachromosomal DNAs (eDNAs) encoded a putative DNA primase (dnaG) gene, a chromosomal gene required for DNA replication and which contains the conserved topoisomerase/primase domain. We speculate that the replication mechanism for the TBB phytoplasma eDNA involves the dnaG gene instead of the rep gene. The Ca. P. australiense eDNA (3773 bp) was shown to be circular and contained four open reading frames. The rep gene was encoded on ORF 1 and had homology to both plasmid (pLS1) and geminivirus-like domains. � 2006 Elsevier Inc. All rights reserved.",
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    author = "L Tran-nguyen and Karen Gibb",
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    language = "English",
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    Extrachromosomal DNA isolated from tomato big bud and Candidatus Phytoplasma australiense phytoplasma strains. / Tran-nguyen, L; Gibb, Karen.

    In: Plasmid, Vol. 56, No. 3, 2006, p. 153-166.

    Research output: Contribution to journalArticleResearchpeer-review

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    T1 - Extrachromosomal DNA isolated from tomato big bud and Candidatus Phytoplasma australiense phytoplasma strains

    AU - Tran-nguyen, L

    AU - Gibb, Karen

    PY - 2006

    Y1 - 2006

    N2 - The nucleotide sequences of two extrachromosomal elements from tomato big bud (TBB) and one extrachromosomal element from Candidatus Phytoplasma australiense (Ca. P. australiense) phytoplasmas were determined. Both TBB plasmids (3319 and 4092 bp) contained an open reading frame (?570 bp) with homology to the rolling circle replication initiator protein (Rep). This gene was shorter than the rep genes identified from other phytoplasma plasmids, geminiviruses and bacterial plasmids. Both TBB extrachromosomal DNAs (eDNAs) encoded a putative DNA primase (dnaG) gene, a chromosomal gene required for DNA replication and which contains the conserved topoisomerase/primase domain. We speculate that the replication mechanism for the TBB phytoplasma eDNA involves the dnaG gene instead of the rep gene. The Ca. P. australiense eDNA (3773 bp) was shown to be circular and contained four open reading frames. The rep gene was encoded on ORF 1 and had homology to both plasmid (pLS1) and geminivirus-like domains. � 2006 Elsevier Inc. All rights reserved.

    AB - The nucleotide sequences of two extrachromosomal elements from tomato big bud (TBB) and one extrachromosomal element from Candidatus Phytoplasma australiense (Ca. P. australiense) phytoplasmas were determined. Both TBB plasmids (3319 and 4092 bp) contained an open reading frame (?570 bp) with homology to the rolling circle replication initiator protein (Rep). This gene was shorter than the rep genes identified from other phytoplasma plasmids, geminiviruses and bacterial plasmids. Both TBB extrachromosomal DNAs (eDNAs) encoded a putative DNA primase (dnaG) gene, a chromosomal gene required for DNA replication and which contains the conserved topoisomerase/primase domain. We speculate that the replication mechanism for the TBB phytoplasma eDNA involves the dnaG gene instead of the rep gene. The Ca. P. australiense eDNA (3773 bp) was shown to be circular and contained four open reading frames. The rep gene was encoded on ORF 1 and had homology to both plasmid (pLS1) and geminivirus-like domains. � 2006 Elsevier Inc. All rights reserved.

    KW - DNA primase

    KW - DNA topoisomerase

    KW - extrachromosomal DNA

    KW - helicase

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    KW - bacterial strain

    KW - bud

    KW - candidatus phytoplasma australiense

    KW - DNA isolation

    KW - DNA replication

    KW - Geminivirus

    KW - gene

    KW - nonhuman

    KW - nucleotide sequence

    KW - open reading frame

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    KW - tomato

    KW - Amino Acid Sequence

    KW - Base Sequence

    KW - Blotting, Southern

    KW - Cluster Analysis

    KW - DNA Primase

    KW - DNA Primers

    KW - DNA Replication

    KW - DNA, Bacterial

    KW - Electrophoresis, Gel, Two-Dimensional

    KW - Molecular Sequence Data

    KW - Open Reading Frames

    KW - Phylogeny

    KW - Plasmids

    KW - Sequence Alignment

    KW - Sequence Analysis, DNA

    KW - Sequence Homology

    KW - Species Specificity

    KW - Bacteria (microorganisms)

    KW - Candidatus Phytoplasma

    KW - Candidatus Phytoplasma australiense

    KW - Geminiviridae

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    SP - 153

    EP - 166

    JO - Plasmid

    JF - Plasmid

    SN - 0147-619X

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    ER -