Fungi outcompete bacteria under increased uranium concentration in culture media

Saqib Mumtaz, Claire Streten-Joyce, David Parry, Keith Mcguinness, Ping Lu, Karen Gibb

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    As a key part of water management at the Ranger Uranium Mine (Northern Territory, Australia), stockpile (ore and waste) runoff water was applied to natural woodland on the mine lease in accordance with regulatory requirements. Consequently, the soil in these Land Application Areas (LAAs) presents a range of uranium concentrations. Soil samples were collected from LAAs with different concentrations of uranium and extracts were plated onto LB media containing no (0 ppm), low (3 ppm), medium (250 ppm), high (600 ppm) and very high (1500 ppm) uranium concentrations. These concentrations were similar to the range of measured uranium concentrations in the LAAs soils. Bacteria grew on all plates except for the very high uranium concentrations, where only fungi were recovered. Identifications based on bacterial 16S rRNA sequence analysis showed that the dominant cultivable bacteria belonged to the genus Bacillus. Members of the genera Paenibacillus, Lysinibacillus, Klebsiella, Microbacterium and Chryseobacterium were also isolated from the LAAs soil samples. Fungi were identified by sequence analysis of the intergenic spacer region, and members of the genera Aspergillus, Cryptococcus, Penicillium and Curvularia were dominant on plates with very high uranium concentrations. Members of the Paecilomyces and Alternaria were also present but in lower numbers. These findings indicate that fungi can tolerate very high concentrations of uranium and are more resistant than bacteria. Bacteria and fungi isolated at the Ranger LAAs from soils with high concentrations of uranium may have uranium binding capability and hence the potential for uranium bioremediation. � 2013 .
    Original languageEnglish
    Pages (from-to)39-44
    Number of pages6
    JournalJournal of Environmental Radioactivity
    Volume120
    DOIs
    Publication statusPublished - 2013

    Fingerprint

    Uranium
    Fungi
    Culture Media
    uranium
    Bacteria
    fungus
    bacterium
    Soil
    Soils
    soil
    Sequence Analysis
    Chryseobacterium
    Uranium mines
    Paenibacillus
    Paecilomyces
    Northern Territory
    Intergenic DNA
    Cryptococcus
    Bioremediation
    Alternaria

    Cite this

    Mumtaz, Saqib ; Streten-Joyce, Claire ; Parry, David ; Mcguinness, Keith ; Lu, Ping ; Gibb, Karen. / Fungi outcompete bacteria under increased uranium concentration in culture media. In: Journal of Environmental Radioactivity. 2013 ; Vol. 120. pp. 39-44.
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    Fungi outcompete bacteria under increased uranium concentration in culture media. / Mumtaz, Saqib; Streten-Joyce, Claire; Parry, David; Mcguinness, Keith; Lu, Ping; Gibb, Karen.

    In: Journal of Environmental Radioactivity, Vol. 120, 2013, p. 39-44.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Fungi outcompete bacteria under increased uranium concentration in culture media

    AU - Mumtaz, Saqib

    AU - Streten-Joyce, Claire

    AU - Parry, David

    AU - Mcguinness, Keith

    AU - Lu, Ping

    AU - Gibb, Karen

    PY - 2013

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    AB - As a key part of water management at the Ranger Uranium Mine (Northern Territory, Australia), stockpile (ore and waste) runoff water was applied to natural woodland on the mine lease in accordance with regulatory requirements. Consequently, the soil in these Land Application Areas (LAAs) presents a range of uranium concentrations. Soil samples were collected from LAAs with different concentrations of uranium and extracts were plated onto LB media containing no (0 ppm), low (3 ppm), medium (250 ppm), high (600 ppm) and very high (1500 ppm) uranium concentrations. These concentrations were similar to the range of measured uranium concentrations in the LAAs soils. Bacteria grew on all plates except for the very high uranium concentrations, where only fungi were recovered. Identifications based on bacterial 16S rRNA sequence analysis showed that the dominant cultivable bacteria belonged to the genus Bacillus. Members of the genera Paenibacillus, Lysinibacillus, Klebsiella, Microbacterium and Chryseobacterium were also isolated from the LAAs soil samples. Fungi were identified by sequence analysis of the intergenic spacer region, and members of the genera Aspergillus, Cryptococcus, Penicillium and Curvularia were dominant on plates with very high uranium concentrations. Members of the Paecilomyces and Alternaria were also present but in lower numbers. These findings indicate that fungi can tolerate very high concentrations of uranium and are more resistant than bacteria. Bacteria and fungi isolated at the Ranger LAAs from soils with high concentrations of uranium may have uranium binding capability and hence the potential for uranium bioremediation. � 2013 .

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    KW - Australia

    KW - Bioavailability

    KW - Chryseobacterium

    KW - Cultivable bacteria

    KW - Culture media

    KW - Genus bacillus

    KW - High concentration

    KW - Intergenic spacer regions

    KW - Key parts

    KW - Klebsiella

    KW - Land applications

    KW - Microbacterium

    KW - Northern territories

    KW - Paecilomyces

    KW - Paenibacillus

    KW - Regulatory requirements

    KW - Runoff water

    KW - Sequence analysis

    KW - Soil sample

    KW - Uranium bindings

    KW - Uranium concentration

    KW - Bacteria

    KW - Bacteriology

    KW - Biochemistry

    KW - Bioremediation

    KW - Biotechnology

    KW - Fungi

    KW - RNA

    KW - Soil surveys

    KW - Soils

    KW - Uranium

    KW - Uranium mines

    KW - Water management

    KW - Radioactive wastes

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    KW - bacterium

    KW - bioavailability

    KW - bioremediation

    KW - concentration (composition)

    KW - fungus

    KW - mine waste

    KW - radioactive waste

    KW - water management

    KW - woodland

    KW - article

    KW - Aspergillus

    KW - Bacillus

    KW - bacterial growth

    KW - bacterium isolation

    KW - concentration (parameters)

    KW - Cryptococcus

    KW - culture medium

    KW - Curvularia

    KW - forest

    KW - fungus isolation

    KW - mining

    KW - nonhuman

    KW - Penicillium

    KW - RNA sequence

    KW - sequence analysis

    KW - soil

    KW - Culture Media

    KW - DNA, Bacterial

    KW - DNA, Fungal

    KW - Industrial Waste

    KW - Mining

    KW - RNA, Bacterial

    KW - RNA, Ribosomal, 16S

    KW - Soil Microbiology

    KW - Water Pollutants, Radioactive

    KW - Northern Territory

    KW - Ranger Mine

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    DO - 10.1016/j.jenvrad.2013.01.007

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    JO - Journal of Environmental Radioactivity

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