Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity

M O'Brien; Kevin Freeman; G LUM; A CHENG; Susan Jacups; Bart Currie

Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity

M O'Brien, Kevin Freeman, G LUM, A CHENG, Susan Jacups, Bart Currie

Research output: Contribution to journal › Article › peer-review

Abstract

Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomaUei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively. In a prospective study of 160 consecutive sera samples sent for melioidosis serology, respective specificities were 91, 90, and 69, positive predictive values were 41, 32, and 18, and negative predictive values were 99, 98, and 100%. ICT IgM kits are unreliable for diagnosis of melioidosis, but ICT IgG kits may be useful for diagnosing travelers presenting with possible melioidosis who return from regions where melioidosis is endemic.

Original language	English
Pages (from-to)	2239-2240
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	42
Issue number	5
Publication status	Published - 2004

Cite this

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title = "Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity",

abstract = "Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomaUei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively. In a prospective study of 160 consecutive sera samples sent for melioidosis serology, respective specificities were 91, 90, and 69, positive predictive values were 41, 32, and 18, and negative predictive values were 99, 98, and 100%. ICT IgM kits are unreliable for diagnosis of melioidosis, but ICT IgG kits may be useful for diagnosing travelers presenting with possible melioidosis who return from regions where melioidosis is endemic.",

keywords = "immunoglobulin G antibody, immunoglobulin M antibody, analytical equipment, antibody detection, article, Burkholderia pseudomallei, chromatography, diagnostic test, endemic disease, evaluation, hemagglutination, human, immunoassay, immunochromatography, major clinical study, melioidosis, nonhuman, prediction, priority journal, sensitivity and specificity, serology, Antibodies, Bacterial, Hemagglutination Tests, Humans, Immunoassay, Immunoglobulin G, Immunoglobulin M, Melioidosis, Northern Territory, Predictive Value of Tests, Prospective Studies, Sensitivity and Specificity, Travel, Burkholderia, Negibacteria",

author = "M O'Brien and Kevin Freeman and G LUM and A CHENG and Susan Jacups and Bart Currie",

year = "2004",

language = "English",

volume = "42",

pages = "2239--2240",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

TY - JOUR

T1 - Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity

AU - O'Brien, M

AU - Freeman, Kevin

AU - LUM, G

AU - CHENG, A

AU - Jacups, Susan

AU - Currie, Bart

PY - 2004

Y1 - 2004

N2 - Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomaUei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively. In a prospective study of 160 consecutive sera samples sent for melioidosis serology, respective specificities were 91, 90, and 69, positive predictive values were 41, 32, and 18, and negative predictive values were 99, 98, and 100%. ICT IgM kits are unreliable for diagnosis of melioidosis, but ICT IgG kits may be useful for diagnosing travelers presenting with possible melioidosis who return from regions where melioidosis is endemic.

AB - Immunochromatographic test (ICT) kits for the rapid detection of immunoglobulin G (IgG) and IgM antibodies to Burkholderia pseudomaUei were compared to the indirect hemagglutination (IHA) assay. In 138 culture-confirmed melioidosis cases, sensitivities were 80, 77, and 88% for IHA, ICT IgG, and ICT IgM, respectively. In a prospective study of 160 consecutive sera samples sent for melioidosis serology, respective specificities were 91, 90, and 69, positive predictive values were 41, 32, and 18, and negative predictive values were 99, 98, and 100%. ICT IgM kits are unreliable for diagnosis of melioidosis, but ICT IgG kits may be useful for diagnosing travelers presenting with possible melioidosis who return from regions where melioidosis is endemic.

KW - immunoglobulin G antibody

KW - immunoglobulin M antibody

KW - analytical equipment

KW - antibody detection

KW - article

KW - Burkholderia pseudomallei

KW - chromatography

KW - diagnostic test

KW - endemic disease

KW - evaluation

KW - hemagglutination

KW - human

KW - immunoassay

KW - immunochromatography

KW - major clinical study

KW - melioidosis

KW - nonhuman

KW - prediction

KW - priority journal

KW - sensitivity and specificity

KW - serology

KW - Antibodies, Bacterial

KW - Hemagglutination Tests

KW - Humans

KW - Immunoassay

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Melioidosis

KW - Northern Territory

KW - Predictive Value of Tests

KW - Prospective Studies

KW - Sensitivity and Specificity

KW - Travel

KW - Burkholderia

KW - Negibacteria

M3 - Article

VL - 42

SP - 2239

EP - 2240

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 5

ER -

Further evaluation of a rapid diagnostic test for melioidosis in an area of endemicity

Abstract

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