Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia

Hussein Mohammed, Kedir Hassen, Ashenafi Assefa, Kalkidan Mekete, Gemechu Tadesse, Girum Taye, Robert J. Commons

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Background: Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. 

    Methods: Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. 

    Results: A total of 118 patients were enrolled including 95 (80.5%) with uncomplicated infection and 23 (19.5%) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2%) and severe disease 12 (52.2%). In msp-2, FC27 was detected in 55 (57.9%) of uncomplicated infections and IC/3D7 in 14 (60.9%) of severe infections. 76 (64.4%) of the 118 isolates contained multiple genotypes; 56 (58.9%) in uncomplicated infections and 19 (82.6%) in severe infections. The overall of multiplicity of infection was 2.2 (95% CI 1.98-2.42) with 1.4 (95% CI 1.23-1.55) and 1.7 (95% CI 1.49-1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95% CI 2.61-3.47) versus 2.0 (95% CI 1.83-2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). 

    Conclusion: Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas.

    Original languageEnglish
    Article number413
    Pages (from-to)1-8
    Number of pages8
    JournalMalaria Journal
    Volume18
    DOIs
    Publication statusPublished - 10 Dec 2019

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    Ethiopia
    Plasmodium falciparum
    Malaria
    Infection
    Genes
    Falciparum Malaria
    Parasites
    Genotype
    Health Facilities
    Electrophoresis

    Cite this

    Mohammed, Hussein ; Hassen, Kedir ; Assefa, Ashenafi ; Mekete, Kalkidan ; Tadesse, Gemechu ; Taye, Girum ; Commons, Robert J. / Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia. In: Malaria Journal. 2019 ; Vol. 18. pp. 1-8.
    @article{e66d0d29140847c99e55e9d888415a08,
    title = "Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia",
    abstract = "Background: Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. Methods: Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. Results: A total of 118 patients were enrolled including 95 (80.5{\%}) with uncomplicated infection and 23 (19.5{\%}) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2{\%}) and severe disease 12 (52.2{\%}). In msp-2, FC27 was detected in 55 (57.9{\%}) of uncomplicated infections and IC/3D7 in 14 (60.9{\%}) of severe infections. 76 (64.4{\%}) of the 118 isolates contained multiple genotypes; 56 (58.9{\%}) in uncomplicated infections and 19 (82.6{\%}) in severe infections. The overall of multiplicity of infection was 2.2 (95{\%} CI 1.98-2.42) with 1.4 (95{\%} CI 1.23-1.55) and 1.7 (95{\%} CI 1.49-1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95{\%} CI 2.61-3.47) versus 2.0 (95{\%} CI 1.83-2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). Conclusion: Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas.",
    keywords = "Disease severity, Ethiopia, Genetic diversity, Merozoite surface protein, Plasmodium falciparum",
    author = "Hussein Mohammed and Kedir Hassen and Ashenafi Assefa and Kalkidan Mekete and Gemechu Tadesse and Girum Taye and Commons, {Robert J.}",
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    Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia. / Mohammed, Hussein; Hassen, Kedir; Assefa, Ashenafi; Mekete, Kalkidan; Tadesse, Gemechu; Taye, Girum; Commons, Robert J.

    In: Malaria Journal, Vol. 18, 413, 10.12.2019, p. 1-8.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Genetic diversity of Plasmodium falciparum isolates from patients with uncomplicated and severe malaria based on msp-1 and msp-2 genes in Gublak, North West Ethiopia

    AU - Mohammed, Hussein

    AU - Hassen, Kedir

    AU - Assefa, Ashenafi

    AU - Mekete, Kalkidan

    AU - Tadesse, Gemechu

    AU - Taye, Girum

    AU - Commons, Robert J.

    PY - 2019/12/10

    Y1 - 2019/12/10

    N2 - Background: Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. Methods: Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. Results: A total of 118 patients were enrolled including 95 (80.5%) with uncomplicated infection and 23 (19.5%) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2%) and severe disease 12 (52.2%). In msp-2, FC27 was detected in 55 (57.9%) of uncomplicated infections and IC/3D7 in 14 (60.9%) of severe infections. 76 (64.4%) of the 118 isolates contained multiple genotypes; 56 (58.9%) in uncomplicated infections and 19 (82.6%) in severe infections. The overall of multiplicity of infection was 2.2 (95% CI 1.98-2.42) with 1.4 (95% CI 1.23-1.55) and 1.7 (95% CI 1.49-1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95% CI 2.61-3.47) versus 2.0 (95% CI 1.83-2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). Conclusion: Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas.

    AB - Background: Malaria infection can present with a wide variety of symptoms, ranging from mild to severe. Plasmodium falciparum isolates in uncomplicated and severe malaria infections may have different parasite genetic profiles. This study was conducted to assess differences in genetic diversity and allelic frequencies in P. falciparum isolates according to malaria severity and age of patients in the Gublack area, northwest Ethiopia. Methods: Cross-sectional health facility-based study conducted in Gublak, Ethiopia between July, 2017 and October, 2017. Symptomatic P. falciparum malaria patients with microscopically-confirmed infection were enrolled. Parasite DNA was extracted from filter paper blood spots and the polymorphic regions of the msp-1 and msp-2 genes were genotyped using allele-specific nested-PCR with fragment analysis by gel electrophoresis. Results: A total of 118 patients were enrolled including 95 (80.5%) with uncomplicated infection and 23 (19.5%) with severe disease. In msp-1, the K1 allelic family was similarly prevalent in uncomplicated 42 (44.2%) and severe disease 12 (52.2%). In msp-2, FC27 was detected in 55 (57.9%) of uncomplicated infections and IC/3D7 in 14 (60.9%) of severe infections. 76 (64.4%) of the 118 isolates contained multiple genotypes; 56 (58.9%) in uncomplicated infections and 19 (82.6%) in severe infections. The overall of multiplicity of infection was 2.2 (95% CI 1.98-2.42) with 1.4 (95% CI 1.23-1.55) and 1.7 (95% CI 1.49-1.86) for msp-1 and msp-2, respectively. Multiplicity of infection was significantly higher in severe than uncomplicated infections (3.0 (95% CI 2.61-3.47) versus 2.0 (95% CI 1.83-2.23), respectively, p = 0.001). There was no difference in multiplicity of infection across age groups (p = 0.104). Conclusion: Patients with severe malaria were more likely to have multiclonal infections. Further studies are needed to describe the association between P. falciparum genotypes and malaria severity in different malaria transmission areas.

    KW - Disease severity

    KW - Ethiopia

    KW - Genetic diversity

    KW - Merozoite surface protein

    KW - Plasmodium falciparum

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    U2 - 10.1186/s12936-019-3039-9

    DO - 10.1186/s12936-019-3039-9

    M3 - Article

    VL - 18

    SP - 1

    EP - 8

    JO - Malaria Journal

    JF - Malaria Journal

    SN - 1475-2875

    M1 - 413

    ER -