Genetic epidemiology of Sarcoptes scabiei (Acari

Sarcoptidae) in northern Australia

Shelley Walton, Annette Marie Dougall, Susan Pizzutto, Deborah Holt, D Taplin, Larry Arlian, Marjorie Morgan, Bart Currie, David J Kemp

    Research output: Contribution to journalArticleResearchpeer-review

    Abstract

    Utilising three hypervariable microsatellite markers we have previously shown that scabies mites on people are genetically distinct from those on dogs in sympatric populations in northern Australia. This had important ramifications on the formulation of public health control policies. In contrast phylogenetic analyses using mitochondrial markers on scabies mites infecting multiple animal hosts elsewhere in the world could not differentiate any genetic variation between mite haplotype and host species. Here we further analyse the intra-specific relationship of Sarcoptes scabiei var. hominis with S. scabiei var. canis by using both mitochondrial DNA and an expanded nuclear microsatellite marker system. Phylogenetic studies using sequences from the mitochondrial genes coding for 16S rRNA and Cytochrome Oxidase subunit I demonstrated significant relationships between S. scabiei MtDNA haplotypes, host species and geographical location. Multi-locus genotyping using 15 microsatellite markers substantiated previous data that gene flow between scabies mite populations on human and dog hosts is extremely rare in northern Australia. These data clearly support our previous contention that control programs for human scabies in endemic areas with sympatric S. scabiei var. hominis and var. canis populations must focus on human-to-human transmission. The genetic division of dog and human derived scabies mites also has important implications in vaccine and diagnostic test development as well as the emergence and monitoring of drug resistance in S. scabiei in northern Australia. � 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
    Original languageEnglish
    Pages (from-to)839-849
    Number of pages11
    JournalInternational Journal for Parasitology
    Volume34
    Issue number7
    Publication statusPublished - 2004

    Fingerprint

    Sarcoptidae
    Sarcoptes scabiei
    Scabies
    Molecular Epidemiology
    Mites
    Microsatellite Repeats
    Dogs
    Haplotypes
    Population
    Parasitology
    Mitochondrial Genes
    Gene Flow
    Electron Transport Complex IV
    Public Policy
    Health Policy
    Mitochondrial DNA
    Routine Diagnostic Tests
    Drug Resistance
    Vaccines
    Public Health

    Cite this

    Walton, S., Dougall, A. M., Pizzutto, S., Holt, D., Taplin, D., Arlian, L., ... Kemp, D. J. (2004). Genetic epidemiology of Sarcoptes scabiei (Acari: Sarcoptidae) in northern Australia. International Journal for Parasitology, 34(7), 839-849.
    Walton, Shelley ; Dougall, Annette Marie ; Pizzutto, Susan ; Holt, Deborah ; Taplin, D ; Arlian, Larry ; Morgan, Marjorie ; Currie, Bart ; Kemp, David J. / Genetic epidemiology of Sarcoptes scabiei (Acari : Sarcoptidae) in northern Australia. In: International Journal for Parasitology. 2004 ; Vol. 34, No. 7. pp. 839-849.
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    abstract = "Utilising three hypervariable microsatellite markers we have previously shown that scabies mites on people are genetically distinct from those on dogs in sympatric populations in northern Australia. This had important ramifications on the formulation of public health control policies. In contrast phylogenetic analyses using mitochondrial markers on scabies mites infecting multiple animal hosts elsewhere in the world could not differentiate any genetic variation between mite haplotype and host species. Here we further analyse the intra-specific relationship of Sarcoptes scabiei var. hominis with S. scabiei var. canis by using both mitochondrial DNA and an expanded nuclear microsatellite marker system. Phylogenetic studies using sequences from the mitochondrial genes coding for 16S rRNA and Cytochrome Oxidase subunit I demonstrated significant relationships between S. scabiei MtDNA haplotypes, host species and geographical location. Multi-locus genotyping using 15 microsatellite markers substantiated previous data that gene flow between scabies mite populations on human and dog hosts is extremely rare in northern Australia. These data clearly support our previous contention that control programs for human scabies in endemic areas with sympatric S. scabiei var. hominis and var. canis populations must focus on human-to-human transmission. The genetic division of dog and human derived scabies mites also has important implications in vaccine and diagnostic test development as well as the emergence and monitoring of drug resistance in S. scabiei in northern Australia. � 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.",
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    author = "Shelley Walton and Dougall, {Annette Marie} and Susan Pizzutto and Deborah Holt and D Taplin and Larry Arlian and Marjorie Morgan and Bart Currie and Kemp, {David J}",
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    Walton, S, Dougall, AM, Pizzutto, S, Holt, D, Taplin, D, Arlian, L, Morgan, M, Currie, B & Kemp, DJ 2004, 'Genetic epidemiology of Sarcoptes scabiei (Acari: Sarcoptidae) in northern Australia', International Journal for Parasitology, vol. 34, no. 7, pp. 839-849.

    Genetic epidemiology of Sarcoptes scabiei (Acari : Sarcoptidae) in northern Australia. / Walton, Shelley; Dougall, Annette Marie; Pizzutto, Susan; Holt, Deborah; Taplin, D; Arlian, Larry; Morgan, Marjorie; Currie, Bart; Kemp, David J.

    In: International Journal for Parasitology, Vol. 34, No. 7, 2004, p. 839-849.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Genetic epidemiology of Sarcoptes scabiei (Acari

    T2 - Sarcoptidae) in northern Australia

    AU - Walton, Shelley

    AU - Dougall, Annette Marie

    AU - Pizzutto, Susan

    AU - Holt, Deborah

    AU - Taplin, D

    AU - Arlian, Larry

    AU - Morgan, Marjorie

    AU - Currie, Bart

    AU - Kemp, David J

    PY - 2004

    Y1 - 2004

    N2 - Utilising three hypervariable microsatellite markers we have previously shown that scabies mites on people are genetically distinct from those on dogs in sympatric populations in northern Australia. This had important ramifications on the formulation of public health control policies. In contrast phylogenetic analyses using mitochondrial markers on scabies mites infecting multiple animal hosts elsewhere in the world could not differentiate any genetic variation between mite haplotype and host species. Here we further analyse the intra-specific relationship of Sarcoptes scabiei var. hominis with S. scabiei var. canis by using both mitochondrial DNA and an expanded nuclear microsatellite marker system. Phylogenetic studies using sequences from the mitochondrial genes coding for 16S rRNA and Cytochrome Oxidase subunit I demonstrated significant relationships between S. scabiei MtDNA haplotypes, host species and geographical location. Multi-locus genotyping using 15 microsatellite markers substantiated previous data that gene flow between scabies mite populations on human and dog hosts is extremely rare in northern Australia. These data clearly support our previous contention that control programs for human scabies in endemic areas with sympatric S. scabiei var. hominis and var. canis populations must focus on human-to-human transmission. The genetic division of dog and human derived scabies mites also has important implications in vaccine and diagnostic test development as well as the emergence and monitoring of drug resistance in S. scabiei in northern Australia. � 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

    AB - Utilising three hypervariable microsatellite markers we have previously shown that scabies mites on people are genetically distinct from those on dogs in sympatric populations in northern Australia. This had important ramifications on the formulation of public health control policies. In contrast phylogenetic analyses using mitochondrial markers on scabies mites infecting multiple animal hosts elsewhere in the world could not differentiate any genetic variation between mite haplotype and host species. Here we further analyse the intra-specific relationship of Sarcoptes scabiei var. hominis with S. scabiei var. canis by using both mitochondrial DNA and an expanded nuclear microsatellite marker system. Phylogenetic studies using sequences from the mitochondrial genes coding for 16S rRNA and Cytochrome Oxidase subunit I demonstrated significant relationships between S. scabiei MtDNA haplotypes, host species and geographical location. Multi-locus genotyping using 15 microsatellite markers substantiated previous data that gene flow between scabies mite populations on human and dog hosts is extremely rare in northern Australia. These data clearly support our previous contention that control programs for human scabies in endemic areas with sympatric S. scabiei var. hominis and var. canis populations must focus on human-to-human transmission. The genetic division of dog and human derived scabies mites also has important implications in vaccine and diagnostic test development as well as the emergence and monitoring of drug resistance in S. scabiei in northern Australia. � 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

    KW - cytochrome c oxidase

    KW - mitochondrial DNA

    KW - RNA 16S

    KW - epidemiology

    KW - genetic variation

    KW - intraspecific variation

    KW - parasite control

    KW - parasitology

    KW - phylogenetics

    KW - article

    KW - Australia

    KW - controlled study

    KW - dog

    KW - female

    KW - gene flow

    KW - genetic epidemiology

    KW - genotype

    KW - geography

    KW - haplotype

    KW - male

    KW - microsatellite marker

    KW - mitochondrial genetics

    KW - nonhuman

    KW - nucleotide sequence

    KW - phylogeny

    KW - Sarcoptes scabiei

    KW - scabies

    KW - Animals

    KW - Base Sequence

    KW - DNA, Mitochondrial

    KW - Dogs

    KW - Electron Transport Complex IV

    KW - Female

    KW - Haplotypes

    KW - Host-Parasite Relations

    KW - Humans

    KW - Male

    KW - Microsatellite Repeats

    KW - Molecular Sequence Data

    KW - Northern Territory

    KW - Phylogeny

    KW - Ploidies

    KW - RNA, Ribosomal, 16S

    KW - Australasia

    KW - Acari

    KW - Animalia

    KW - Canis

    KW - Canis familiaris

    KW - Psoroptes cervinus

    KW - Sarcoptes scabiei type hominis

    KW - Sarcoptidae

    M3 - Article

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    SP - 839

    EP - 849

    JO - International Journal for Parasitology

    JF - International Journal for Parasitology

    SN - 0020-7519

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    ER -

    Walton S, Dougall AM, Pizzutto S, Holt D, Taplin D, Arlian L et al. Genetic epidemiology of Sarcoptes scabiei (Acari: Sarcoptidae) in northern Australia. International Journal for Parasitology. 2004;34(7):839-849.