Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups: A cohort study

Heidi Smith-Vaughan; Jemima Beissbarth; Jacinta  Bowman; Kim Hare; Erin  PRICE; Janessa Pickering; Deborah Lehmann; Anne Chang; Peter Morris; Robyn Marsh; Amanda Leach

doi:10.1186/s41479-016-0013-y

Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups: A cohort study

Heidi Smith-Vaughan, Jemima Beissbarth, Jacinta Bowman, Kim Hare, Erin PRICE, Janessa Pickering, Deborah Lehmann, Anne Chang, Peter Morris, Robyn Marsh, Amanda Leach

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Abstract

Non-typeable Haemophilus influenzae (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world.NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Pneumonia
Volume	8
Issue number	13
DOIs	https://doi.org/10.1186/s41479-016-0013-y
Publication status	Published - 2016

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11603643-oaFinal published version, 885 KBLicence: CC BY

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title = "Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups: A cohort study",

abstract = "Non-typeable Haemophilus influenzae (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world.NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.",

author = "Heidi Smith-Vaughan and Jemima Beissbarth and Jacinta Bowman and Kim Hare and Erin PRICE and Janessa Pickering and Deborah Lehmann and Anne Chang and Peter Morris and Robyn Marsh and Amanda Leach",

note = "Australian National Health and Medical Research Council (NHMRC grant numbers 212044, 436023, 100009, 1023781)",

year = "2016",

doi = "10.1186/s41479-016-0013-y",

language = "English",

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journal = "Pneumonia",

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T1 - Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups

T2 - A cohort study

AU - Smith-Vaughan, Heidi

AU - Beissbarth, Jemima

AU - Bowman, Jacinta

AU - Hare, Kim

AU - PRICE, Erin

AU - Pickering, Janessa

AU - Lehmann, Deborah

AU - Chang, Anne

AU - Morris, Peter

AU - Marsh, Robyn

AU - Leach, Amanda

N1 - Australian National Health and Medical Research Council (NHMRC grant numbers 212044, 436023, 100009, 1023781)

PY - 2016

Y1 - 2016

N2 - Non-typeable Haemophilus influenzae (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world.NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.

AB - Non-typeable Haemophilus influenzae (NTHi)-associated ear and respiratory diseases (including pneumonia) represent a major health burden in many parts of the world.NTHi strains retrieved from the upper airways commonly reflect those found in the lower airways. Despite growing genomic and genotyping data on NTHi, there remains a limited understanding of global and regional NTHi population structures. The aim of this study was to determine whether nasopharyngeal carriage in four Australian paediatric groups at varying risk of NTHi colonisation was dominated by the same NTHi genotypes. Genotyping data generated by PCR-ribotyping were evaluated for 3070 NTHi isolates colonising the nasopharynges of Aboriginal and non-Aboriginal children enrolled in four longitudinal studies in three separate urban and remote regions of Australia. Several NTHi PCR-ribotypes dominated in nasopharyngeal carriage, irrespective of study setting. Principal coordinates analysis confirmed a cluster of common PCR-ribotypes among all cohorts. In conclusion, we identified dominant PCR-ribotypes common to geographically disparate Australian paediatric populations. Future genomic analyses will shed further light on the precise factors underlying the dominance of certain NTHi strains in nasopharyngeal carriage.

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DO - 10.1186/s41479-016-0013-y

M3 - Article

C2 - 28702292

SN - 2200-6133

VL - 8

SP - 1

EP - 6

JO - Pneumonia

JF - Pneumonia

IS - 13

ER -

Geographic consistency in dominant, non-typeable Haemophilus influenzae genotypes colonising four distinct Australian paediatric groups: A cohort study

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