TY - JOUR
T1 - Gold nanoparticle-assisted enhancement in bioactive properties of Australian native plant extracts, Tasmannia lanceolata and Backhousia citriodora
AU - Khandanlou, Roshanak
AU - Murthy, Vinuthaa
AU - Wang, Hao
PY - 2020/7
Y1 - 2020/7
N2 - Green nanotechnology plays a significant role in developing effective treatment strategies for numerous diseases. The biological synthesis of metal nanoparticles (M-NPs) possesses suitable alternatives than chemical techniques. Using plant extract to synthesis M-NPs is an eco-friendly, non-toxic, and cost-effective that are suitable for biological applications and efforts are directed to explore the efficacy of these materials in cancer management. In this study, gold nanoparticles (Au-NPs) were synthesised by following a one-step green synthesis, a reaction between HAuCl4 and biological molecules present in Tasmannia lanceolata leaf extract as a sole agent for both reduction and stabilisation. The characterisation techniques confirmed the successful synthesis of Au-NPs. TEM photograph revealed spherical shape nanoparticles with an average size of 7.10 ± 0.66 nm. The in-vitro cytotoxicity of Au-NPs was performed by analysing the percentage inhibition of cell viability using Resazurin assay on human liver cancer (HepG2), melanoma cancer (MM418 C1) and breast cancer (MCF-7) cell lines and compared with Au-NPs synthesised by using Backhousia citriodora leaf extract. The results showed that biosynthesised Au-NPs displayed greater inhibitory activity towards MCF-7 cancer cells proliferation compared to HepG2 and MM418 cancer cells. In addition, synthesised Au-NPs@ Tasmannia lanceolata leaf extract indicated higher inhibitory activity towards cancer cells compared to Au-NPs@ Backhousia citriodora leaf extract.
AB - Green nanotechnology plays a significant role in developing effective treatment strategies for numerous diseases. The biological synthesis of metal nanoparticles (M-NPs) possesses suitable alternatives than chemical techniques. Using plant extract to synthesis M-NPs is an eco-friendly, non-toxic, and cost-effective that are suitable for biological applications and efforts are directed to explore the efficacy of these materials in cancer management. In this study, gold nanoparticles (Au-NPs) were synthesised by following a one-step green synthesis, a reaction between HAuCl4 and biological molecules present in Tasmannia lanceolata leaf extract as a sole agent for both reduction and stabilisation. The characterisation techniques confirmed the successful synthesis of Au-NPs. TEM photograph revealed spherical shape nanoparticles with an average size of 7.10 ± 0.66 nm. The in-vitro cytotoxicity of Au-NPs was performed by analysing the percentage inhibition of cell viability using Resazurin assay on human liver cancer (HepG2), melanoma cancer (MM418 C1) and breast cancer (MCF-7) cell lines and compared with Au-NPs synthesised by using Backhousia citriodora leaf extract. The results showed that biosynthesised Au-NPs displayed greater inhibitory activity towards MCF-7 cancer cells proliferation compared to HepG2 and MM418 cancer cells. In addition, synthesised Au-NPs@ Tasmannia lanceolata leaf extract indicated higher inhibitory activity towards cancer cells compared to Au-NPs@ Backhousia citriodora leaf extract.
KW - Backhousia citriodora
KW - Cancer cell lines
KW - Gold nanoparticles
KW - Resazurin assay
KW - Tasmannia lanceolata
UR - http://www.scopus.com/inward/record.url?scp=85082748566&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2020.110922
DO - 10.1016/j.msec.2020.110922
M3 - Article
C2 - 32409072
AN - SCOPUS:85082748566
VL - 112
SP - 1
EP - 10
JO - Materials Science and Engineering C: Materials for Biological Applications
JF - Materials Science and Engineering C: Materials for Biological Applications
SN - 0928-4931
M1 - 110922
ER -