@article{a508b7d9c0e449ce884bea11dab69e1e,
title = "Haemophilus influenzae isolates survive for up to 20years at -70°C in skim milk tryptone glucose glycerol broth (STGGB) if thawing is avoided during re-culture",
abstract = "Haemophilus influenzae remains a major cause of disease worldwide requiring continued study. Recently, isolates of Streptococcus pneumoniae and Moraxella catarrhalis, but not H. influenzae, were reported to survive long-term ultra-freeze storage in STGGB. We show that nontypeable H. influenzae isolates survive for up to 20. years when thawing is avoided.",
keywords = "glycerol, virulence factor, Article, chronic obstructive lung disease, controlled study, disease association, Haemophilus influenzae, lung lavage, milk, Moraxella catarrhalis, nonhuman, otitis media, polymerase chain reaction, priority journal, retrospective study, Streptococcus pneumoniae, thawing, virus isolation",
author = "Hare, {K. M.} and Smith-Vaughan, {H. C.} and Leach, {A. J.} and J. Beissbarth",
year = "2015",
month = dec,
day = "1",
doi = "10.1016/j.mimet.2015.10.013",
language = "English",
volume = "119",
pages = "132--133",
journal = "Journal of Microbiological Methods",
issn = "1872-8359",
publisher = "Elsevier",
}